Comparative Clinical Pathology

, Volume 27, Issue 5, pp 1167–1172 | Cite as

Association between hormonal and structural changes of the reproductive tract in estrus dairy cows

  • Behrooz Mihandoost
  • Asghar Mogheiseh
  • Saeed Nazifi
  • Mohammad Rahim Ahmadi
  • Maryam Ansari-Lari
Original Article


Normal cyclic changes in hormonal and ultrasonic echotexture along with estrus are critical for successful breeding in dairy cows. We studied relationships among steroid hormone concentrations and ultrasound changes of uterus and ovaries just before insemination in heat dairy cows. Eighty-nine estrus dairy cows were selected for transrectal examination of uterus and ovaries, and blood sampling just before AI. Ultrasound characteristics of the uterus and ovarian structures were recorded. The concentration of estrogen, progesterone, and cortisol was measured in serum of estrus cows. Our results showed that there was not any relationship between ultrasound findings of uterus and steroid hormones in heat dairy cows. Also, there was no correlation between serum progesterone, estradiol, and cortisol concentrations with the diameter of dominant follicle. In addition, it was found that there was no correlation between serum progesterone, cortisol concentrations, and the presence of corpus luteum (CL) on ovary in heat cows, but there was a negative significant relationship between the presence of CL and estradiol concentration (r = − 0.21, P = 0.04). Association between ultrasound characteristics of the uterus and steroid hormone (estradiol, progesterone, and cortisol) concentrations were not significant and considerable in estrus dairy cows.


Ultrasonography Uterus Ovaries Estradiol Estrus 



The authors thank Pegah Company (Shiraz, Iran) for their permission to use their herd in the experiment and thank their staff for the preparation of the cows involved in this study and their assistance during the project.


This study was supported by the School of Veterinary Medicine, Shiraz University (grant number 94GCU2M154630).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Sciences, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Department of Animal and Food Health, School of Veterinary MedicineShiraz UniversityShirazIran

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