Skip to main content

Advertisement

SpringerLink
Log in

Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl4-induced nephrotoxicity in mice

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

In this study, Glycyrrhiza glabra L (GG) aqueous extract was extracted to evaluate its nephroprotective effect on renal structural, hematological, and biochemical changes in CCl4-induced nephrotoxicity in male mice. Fifty male mice were divided into five groups (n = 10); group I served as control, received 1 mL/kg olive oil intraperitoneally and 0.5 mL distilled water through gavage. Group II served as untreated group, received 1 mg/kg CCl4 mixed with olive oil in the ratio of 1:1 intraperitoneally and 0.5 mL distilled water orally. Groups III, IV, V, and VI received CCl4 mixed with olive oil in the ratio of 1:1 intraperitoneally and 30, 90, and 270 mg/kg of GG through gavage for 45 consecutive days. At 45th day, the mice were dissected; blood and kidney samples collected for hematological, biochemical, and histological parameter analysis. The data of this study were analyzed by the SPSS-18 software using one-way analysis of variance (ANOVA) followed by Duncan’s post hoc test (p ≤ 0.05). Different doses of GG (especially GG270) could significantly (p ≤ 0.05) reduce the raised levels of urea, creatinine, WBC, and platelet and increased SOD, CAT, and RBC levels as compared to the untreated group. The weight and volume of the renal structures were decreased significantly (p ≤ 0.05) in several doses of GG as compared to the untreated group. In conclusion, GG has nephroprotective effect, thereby reducing the causation of toxicity in experimental mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12

Similar content being viewed by others

References

  • Abei H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 673–684

    Chapter  Google Scholar 

  • Alia M, Horcajo C, Bravo L, Goya L (2003) Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. Nutr Res 23(9): 1251–1267.

  • Ammosov S, Litvinenko VI (2003) Triterpenoids of plants of Glycyrrhiza glabra L. and Meristotropis Fisch. Et Mey Genuses. Pharm Chem J 37(2): 83–94.

  • Bicalho MD, Soares DB, Botoni FA, Reis AMM, Martins MAP (2015) Drug-induced nephrotoxicity and dose adjustment recommendations: agreement among four drug information sources. Int J Environ Res Public Health 12: 11227–11240.

  • Braendgaard H, Gundersen HJ (1986) The impact of recent stereological advances on quantitative studies of the nervous system. J Neurosci Methods 18(1–2):39–78

    Article  PubMed  CAS  Google Scholar 

  • Breyer MD, Bottinger E, Brosius FC, Coffman TM, Harris RC, Heilig CW, Sharma K, AMDCC (2005) Mouse models of diabetic nephropathy. J Am Soc Nephrol 16(1); 27–45.

  • Brosius FC, Alpers CE, Bottinger EP, Breyer MD, Coffman TM, Gurley SB, Harris RC, Kakoki M, Kretzler M, Leiter EH, Levi M, McIndoe RA, Sharma K, Smithies O, Susztak K, Takahashi N, Takahashi T (2009) Mouse models of diabetic nephropathy. J Am Soc Nephrol 20(12): 2503–2512.

  • Dhodi DK, Bhagat SB, Pathak D, Patel SB (2014) Drug-induced nephrotoxicity. Int J Basic Clin Pharmacol 3(4): 591–597.

  • Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2016). Antibacterial effect and phytochemical screening of essential oil of Pimpinella anisum against Escherichia coli O157:H7 and Staphylococcus aureus. Int J Current Med Pharm Res 7(6): 367–371.

  • Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2017) Medicinal plants: antibacterial effects and chemical composition of essential oil of Foeniculum vulgare. Int J Curr Pharm Rew Res 8(1):13–17

    Google Scholar 

  • Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263

    Article  Google Scholar 

  • Goodarzi N, Zangeneh MM, Zangeneh A, Najafi F, Tahvilian R (2017) Protective effects of ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced hepatotoxicity in mice. J Rafsanjan Univ Med Sci 16(3): 227–238.

  • Goodarzi N, Zangeneh MM, Zangeneh A (2018) The effect of ethanolic extract of Allium saralicum R.M. Fritsch on diabetic hepatopathy in male mice. Sci Res J Shahed Univ 25:21–30

    Google Scholar 

  • Goorani S, Zangeneh MM, Zangeneh A, Poorshamohammad C, Abiari M, Moradi R, Najafi F, Tahvilian R (2018) Study of wound healing potential of Stevia rebaudiana ethanol extract in male rats. Res J Pharmacogn 5(1):23–30

    Google Scholar 

  • Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A (1992) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis.Acta Pathol Microbiol Immunol Scand 96(5): 379–394.

  • Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26 (2): 455–463.

  • Huff GR, Huff WE, Balog JM, Rath NC, Anthony NB, Nestor KE (2005) Stress response differences and disease susceptibility reflected by heterophil to lymphocyte ratio in Turkeys selected for increased body weight. Poult Sci 84(5):709–717.

  • Irani M, Sarmadi M, Bernard F, Ebrahimipour GH, Bazarnov HS (2010) Leaves antimicrobial activity of Glycyrrhiza glabra L. Iran J Pharm Res 9(4):425–428

    PubMed  PubMed Central  Google Scholar 

  • Kitabchi AE, Umpierrez GE, Miles JM, Fisher JN (2009) Hyperglycemic crises in adult patients with diabetes. Diabetes Care 32(7): 1335–1343.

  • Larson CT, Gross WB, Davis JW (1985) Social stress and resistance of chicken and swine to Staphylococcus aureus challenge infections. Can J Comp Med 49(2): 208–210.

  • Le-Devehat C, Khodabandehlou T, Vimeux M (2001) Impaired hemorheological properties in diabetic patients with lower limb arterial ischaemia. Clin Hemorheol Microcirc 25(2):43–48.

  • Mandarim-de-Lacerda CA (2003) Stereological tools in biomedical research. An Acad Bras Cienc 75(4): 469–486.

  • Martin JP Jr, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255(2):329–336

    Article  PubMed  CAS  Google Scholar 

  • Mishra S, Ranjan-Pani S, Sahoo S (2014) Anti-nephrotoxic activity of some medicinal plants from tribal rich pockets of Odisha. Pharmacogn Res 6(3):210–217

    Article  Google Scholar 

  • Mohana-Lakshmi S, Usha-Kiran-Reddy T, Sandhya-Rani KS (2012) A review on medicinal plants for nephroprotective activity. Asian J Pharm Clin Res 5(4):8–14

    Google Scholar 

  • Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Faizi S, Zoalfaghari M, Marabi A (2017) Study a plant extract as an antibacterial agent. Int J Curr Med Pharm Res 3(2):1360–1362

    Google Scholar 

  • Najafi F, Goodarzi N, Zangeneh MM, Zangeneh A, Hagh-Nazari L (2017) Antidiabetic and hepatoprotective effects of bitter fraction of Stevia rebaudiana alcoholic extract on streptozotocin-induced diabetic male mice. J Rafsanjan Univ Med Sci 16(6):493–504

    Google Scholar 

  • Palm F, Ortster H, Hansell P, Liss P, Carlsson PO (2004) Differentiating between effects of streptozotocin per se and subsequent hyperglycemia on renal function and metabolism in the streptozotocin diabetic rat model. Diabetes Metab Res Rev 20(6): 452–459.

  • Poorshamohammad C, Souri N, Amini Z, Kosari F, Jamshidpour R, Zangeneh MM, Zangeneh A (2017) Cucurbita moschata: a plant with antibacterial properties. Int J Current Med Pharm Res 3(2): 1356–1359.

  • Pooyanmehr M, Zangeneh MM, Zangeneh A, Almasi M (2017) Effect of Verbascum thapsus aqueous extract on Escherichia coli O157:H7. Onl J Vet Res 21(9): 580–583.

  • Saba AB, Oyagbemi AA, Azeez OI (2010) Amelioration of carbon tetrachloride-induced hepatotoxicity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats. Nig J Physiol Sci 25(2): 139–147.

  • Saxena S (2005) Glycyrrhiza glabra: medicine over the millennium. Nat Prod Rad 4(5):358–367

    Google Scholar 

  • Schetz M, Dasta J, Goldstein S, Golper T (2005) Drug-induced acute kidney injury. Curr Opin Crit Care 11(6): 555–565.

  • Sheetal V, Ashlesha K (2011) Glycyrrhiza glabra Linn- “KLITAKA”: a review. Int J Pharma Bio Sci 2(3): 42–51.

  • Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543

    Article  Google Scholar 

  • Swenson MJ (1993) Physiological properties and cellular and chemical constituent of blood. In: Dukes’ physiology of domestic animals. Comstock Publishing Associates, Ithaca and London. 29–32.

  • Tahvilian R, Moradi R, Zhaleh H, Zangeneh MM, Zangeneh A, Yazdani H, Hajialiani M (2017) Chemical composition and screening of antibacterial activity of essential oil of Pistacia khinjuk against two selected pathogenic bacteria. Ann Trop Med Public Health. 10(5): 1159–1164.

  • Tay YC, Wang Y, Kairaitis L, Rangan GK, Zhang C, Harris DCH (2005) Can murine diabetic nephropathy be separated from superimposed acute renal failure? Kidney Int 68(1): 391–398.

  • Tiong HY, Huang P, Xiong S, Li Y, Vathsala A, Zink D (2014) Drug-induced nephrotoxicity: clinical impact and preclinical in vitro models. Mol Pharm 11(7): 1933–1948.

  • Zangeneh MM, Najafi F, Tahvilian R, Salmani S, Haghnazari L, Zangeneh A, Moradi R (2017) Ethnomedicinal plants: in vitro antibacterial effects of ethanolic extract of Stevia rebaudiana. Int J Ayu Pharm Chem 6(1): 251–259.

  • Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F (2018) Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol. https://doi.org/10.1007/s00580-018-2674-9

Download references

Author information

Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Mohammad Mahdi Zangeneh & Akram Zangeneh

  2. Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Mahdi Zangeneh, Akram Zangeneh, Reza Tahvilian & Rohallah Moradi

Authors
  1. Mohammad Mahdi Zangeneh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akram Zangeneh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Reza Tahvilian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rohallah Moradi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohammad Mahdi Zangeneh.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zangeneh, M.M., Zangeneh, A., Tahvilian, R. et al. Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl4-induced nephrotoxicity in mice. Comp Clin Pathol 27, 1119–1126 (2018). https://doi.org/10.1007/s00580-018-2707-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-018-2707-4

Keywords

Search

Navigation