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Innate immunological responses of Asian sea bass, Lates calcarifer (Bloch, 1790) for experimentally challenged Aeromonas hydrophila infection

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Abstract

Modulation of innate immunity was determined in the Aeromonas hydrophila-challenged Asain sea bass, Lates calcarifer. The haemato-immunological parameters were studied in blood serum. Blood was sampled at 3, 6, 12, and 24th h post-challenged infection and control. Histopathological examination of most important organs (ventral musculature tissue, kidney and liver) was studied in the infected fish. The present study results indicated that at early stages of infection, TEC, Hb, PCV, MCV, MCH, and MCHC were not altered significantly, whereas in TLC, the values were fluctuated. Lysozyme, NBT, and protein were significantly increased (P < 0.05) at all the respective time periods than the control. Histopathological results of infected tissues revealed that the myofibrillar necrosis, congested blood vessels, large-sized melano-macrophage with hyper-cellular activity.

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Acknowledgements

We would like to thank RGCA, Sirkali, India, for providing the fish source during the study.

Funding

The authors greatly acknowledge the Ministry of Earth Science (MoES), Government of India, for financial support for the project entitled “Prevalence and impact of ovarian parasitic nematode infestation on the economically important fishes of Tamil Nadu coast including cages and mariculture facilities” (MoES/36/OOIS/Extra/49/2016).

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Authors and Affiliations

  1. Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamilnadu, 608502, India

    Palani Kathirkaman, Gopalakrishnan Ayyaru, Muhil Vannan Serelathan, Vijayapoopathi Singaravel & Thangaraj Gunasekaran

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  1. Palani Kathirkaman
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  2. Gopalakrishnan Ayyaru
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  3. Muhil Vannan Serelathan
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  4. Vijayapoopathi Singaravel
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Correspondence to Gopalakrishnan Ayyaru.

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The authors declare that they have no conflict of interest.

Ethical approval

In the present study, the Asian sea bass (L. calcarifer) used for experimental studies is widely consumed and cultured one in India. Previously, similar work has been carried out similarly. This article does not involve human samples or higher animals during the study.

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This article does not have any studies with human participants.

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Kathirkaman, P., Ayyaru, G., Serelathan, M. et al. Innate immunological responses of Asian sea bass, Lates calcarifer (Bloch, 1790) for experimentally challenged Aeromonas hydrophila infection. Comp Clin Pathol 27, 927–931 (2018). https://doi.org/10.1007/s00580-018-2683-8

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