Abstract
Humulus lupulus L. is a plant from cannabinaceac family which is grown in Iran as well as most regions round the world. The present study aimed at examining effects of hydroalcoholic extract of Humulus lupulus L. hops on blood pictures in male rats. Rats were randomly assigned to seven groups of ten, including one control group (no treatment). All of the six groups under the study were exposed 7 weeks to 25% tween 80° solution (1 ml/kg/day), olive oil (1 ml/kg/day), hydroalcoholic extract of Humulus lupulus L. (H) at 50, 150, or 450 mg/kg/day, as well as Di Ethyl Stilbestrol (DES) (100 μg/Kg BW). Having performed the experiments, the researcher removed rats’ blood serums from the due blood samples and kept them all frozen for usage. After all, results were analyzed by one way-ANOVA statistical method (p < 0.05). Comparing to the control group, it was observed that exposure to Humulus lupulus L. can increase MCH, MCV, Hb, and Hct levels, whereas exposure to DES can significantly reduce Hb and MCHC levels. The present investigation demonstrates that contrary to that of the DES, applying high doses of phytoestrogen can stimulate erythroid colony formation.
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Acknowledgements
I express my thanks to Mrs. Sarraf, and Dr. Nekoeian for their great help during my research.
Funding
This research was supported by the Research Affairs of Shiraz University of Medical Sciences (Grant No. 3001).
This research was supported by Research Center of Shiraz Medical Sciences (Grant No: 3001).
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Najmeh Sadeghi: performing the surgical operation and preparation of the article. N karbalaei: performing the surgical operation and review and submission of the article. Ahad malekzadeh: data analysis.
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The authors declare that they have no conflicts of interest.
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The study was conducted according to the university’s guidelines for the care and use of laboratory animals.
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The results of this study support using (Humulus lupulus L.) can induce the activation of erythroid colonies in male rat so it can be used with cautious in drinks or foods.
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Sadeghi, N., Karbalaei, N. & Malekzadeh, A. The effect of (Humulus lupulus L.) extract on blood factors in male rat. Comp Clin Pathol 27, 841–845 (2018). https://doi.org/10.1007/s00580-018-2670-0
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DOI: https://doi.org/10.1007/s00580-018-2670-0