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Detection of Brucella abortus by immunofluorescence assay using anti-16-kDa outer membrane protein (OMP16) antibody

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Abstract

Brucellosis in humans is one of the most important zoonotic diseases worldwide. This disease causes weakness, fever, pain, sweats, and complications in the liver, spleen, and lymphatic system; Brucellosis requires long-term antibiotic therapy. This study attempts to identify Brucella abortus via an immunofluorescence (IF) test considering antibody against an outer membrane protein with 16-kDa molecular weight (OMP16). The B. abortus OMP16 protein was synthesized through cloning and expressing of the related gene and finally was purified to immunize rabbit in order to produce a polyclonal antibody. Fluorescein isothiocyanate (FITC) was attached to the antibody and the prepared complex was applied to screening of Brucella spp., as an IF test. Polyclonal antibody obtained from the sera of immunized rabbit with the purified OMP16 showed a strong reactivity in ELISA and properly identified the B. abortus strain in IF test. So, this high titer of antibody could be helpful for tracing of B. abortus via laboratory diagnostic tests.

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Abbreviations

B. abortus :

Brucella abortus

FITC:

Fluorescein isothiocyanate

IFA:

Immunofluorescence assay

kDa:

Kilodalton

mAbs:

Monoclonal antibodies

MMs:

Molecular masses

OMP:

Outer membrane protein

PG:

Peptidoglycan

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Acknowledgements

This study was supported by a grant from Iran National Science Foundation (INSF) under grant number 96003341 and Young Researchers Society of Shahid Bahonar University of Kerman.

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Authors and Affiliations

  1. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

    Elham Mohammadi & Mehdi Golchin

  2. Young Researchers Society, Shahid Bahonar University of Kerman, Kerman, Iran

    Elham Mohammadi

  3. Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran

    Mehdi Golchin

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  1. Elham Mohammadi
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  2. Mehdi Golchin
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Correspondence to Elham Mohammadi.

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The authors declare that they have no conflict of interest.

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This study has followed the animal rights in scientific researches and this study has endorsed by the Animal Ethics Association of Kerman University of Medical Sciences.

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Mohammadi, E., Golchin, M. Detection of Brucella abortus by immunofluorescence assay using anti-16-kDa outer membrane protein (OMP16) antibody. Comp Clin Pathol 26, 1299–1304 (2017). https://doi.org/10.1007/s00580-017-2529-9

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  • DOI: https://doi.org/10.1007/s00580-017-2529-9

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