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Blood chemistry, haematology and ileal bacterial counts of broilers fed fermented mixture of cassava root meal and palm kernel cake

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A study on blood chemistry, haematology and ileal bacterial count of broilers fed fermented mixture of grated cassava tubers and palm kernel cake (FCP-mix) as a replacement for maize at 0, 25, 50, 75 and 100% levels of inclusion was undertaken. One hundred and eighty (180) 7-day-old broiler chickens were divided into six groups of 30 birds, and each group replicated thrice. Six experimental diets were formulated with diet 1 containing maize as the major energy source, while diet 6 containing fermented cassava root meal (CRM) and palm kernel cake (PKC) at the ratio of 1:1 as the main energy source. Diets 2, 3, 4 and 5 contained FCP-mix at 25, 50, 75 and 100% as replacement for maize. Diets 1 and 6 served as controls. Each group was assigned to one experimental diet in a completely randomized design. Blood samples for analysis and ileal bacterial count were obtained from each replicate, and data obtained were analysed statistically. Results show the packed cell volume (PCV), haemoglobin (Hb), total red cell count (tRBC), mean cell volume (MCV) and platelets were not significantly affected by the inclusion of FCP-mix based diets, but there are significant variations in total white blood cell (tWBC), lymphocyte and heterophil count. Serum biochemistry data were not affected except for serum calcium, conjugated bilirubin and aspartate aminotransferase (AST) levels that were significantly affected. Mean intestinal Escherichia coli count were reduced at 50 and 75% replacement of maize by FCP-mix whereas at 100% replacement level, salmonella counts decreased by 50%, and E. coli counts became negligible. It is therefore concluded that FCP-mix has some probiotic activity which can be used to enhance performance of broilers and improve product quality.

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  • Aladi NO (2016) Studies on dietary feremented mixture of cassava and palm kernel cake on carcass characteristics of briolers and pigs. PhD Thesis submitted to the Department of Animal Science and Technology, Federal University of Technology, Owerri

  • Aladi NO, Isinguzo C, Okorondu S, Okoli IC, Okeudo NJ (2013) Improvement on the root pulp and palm kernel cake mixture under solid state fermentation. J Agric Tech 9:1111–1124

    CAS  Google Scholar 

  • Chukwukaelo A (2016) Dietary inclusion levels of fermented mixture of cassava root pulp and palm kernel cake on broiler performance. MSc Thesis, Federal University of Technology Owerri, Nigeria

  • Eggum BO (1970) The protein quality of cassava leaves. Br J Nut 24:761–768

    Article  CAS  Google Scholar 

  • Enemor VHA, Anosike JC, Nwoke BEB, Chikezie PC (2005) Serum aminotransferase and bilirubin levels in malaria patients. Int J Nat Appl Sci 1:85–89

    Google Scholar 

  • Etuk EB, Udegbunam AC, Aladi NO, Emenalom OO, Esonu BO (2012) Effects of partial replacement of maize with 2:1:1 combination of plantain peels, yam peels and palm kernel cake in broiler starter diet. Rev Cient UDO Agrícola 12:649–652

    Google Scholar 

  • Heres L, Engel B, Van Knapen F, De Jong F, Wagenaar JA, Urlings HAP (2003) Fermented liquid feed reduces susceptibility of broilers for Salmonalla enteritidis. Poult Sci 82:603–611

    Article  CAS  PubMed  Google Scholar 

  • Iluyemi FB, Hanafi MM, Radziah O, Kamarudin MS (2006) Fungal solid state culture of palm kernel cake. Bioresour Technol 97:477–482

    Article  CAS  PubMed  Google Scholar 

  • Jain NC (1986) Scanning electron micrograph of blood cells. In: Schalm OW, Jain NC, Jain Carol J (eds) Schalm’s veterinary Haematology, 4th edn. Lea and Fabiger, Philadelphia

    Google Scholar 

  • Little TM, Hills JF (1978) Agricultural experimentation: design and analysis. John Wiley and Sons, London

    Google Scholar 

  • Microsoft Excel (2007) Microsoft Corporation

  • Ndlovu T, Chimonyo M, Okoh AI, Muchenje V, Dzama K, Raats JG (2007) Assessing the nutritional status of beef cattle: current practices and future prospects. Afr J Biotech 6:2727–2734

    Article  CAS  Google Scholar 

  • Niba AT, Beal JD, Kudi AC, Brooks PH (2009) Bacterial fermentation in the gastrointestinal tract of non-ruminants: influence of fermented feeds and fermentable carbohydrates. Trop Anim Hlth and Prod 41:1393–1407

    Article  CAS  Google Scholar 

  • Oyewale JO (1987) Haematological and plasma biochemical values of two breeds of domestic fowls in a tropical environment. Anim Tech 38:49–53

    Google Scholar 

  • Schalm JW, Jain NC, Carol EJ (1975) Veterinary Haematology, 3rd edn. Lea and Febrique, Philadelphia, pp 15–81

    Google Scholar 

  • Uchegbu MC, Herbert U, Ogbuewu IP, Nwaodu CH, Esonu BO, Iloeje MU (2011) Growth performance and economy of replacing maize with Jack bean and cassava root meal in broiler finisher rations. Online J Anim Feed Res 1:160–165

    Google Scholar 

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Correspondence to I. P. Ogbuewu.

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The authors declare that they have no conflict of interest.

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This paper was in compliance to all the guidelines of the animal ethics committee of the Federal University of Technology Owerri, Nigeria.

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Aladi, N.O., Chukwukaelo, A.A., Okeudo, N.J. et al. Blood chemistry, haematology and ileal bacterial counts of broilers fed fermented mixture of cassava root meal and palm kernel cake. Comp Clin Pathol 26, 1273–1278 (2017).

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