Abstract
Oil and fatty acids from the flowers and stems of Clerodendrum volubile were investigated for their antioxidative burst activities. The oils were methylated to obtain fatty acid methyl esters (FAMEs). The oils and fatty acids were then subjected to GC-MS analysis to identify the compounds present. Their inhibitory potentials on oxidative burst were investigated on blood phagocytes using the luminol-amplified chemiluminescence technique after activation with serum opsonized zymosan. Succinate derivatives were observed to be the most predominant compounds. C. volubile stem fatty acids showed potent inhibitory activity (IC50 = 18.52 ± 0.12) on blood ROS production. No cytotoxic effect was observed on treatment of CC-1 normal cell lines with the fatty acids. Oral toxicity prediction of the identified fatty acids of C. volubile stem via ProTox revealed their safety on swallowing. These results suggest the antioxidative burst activity of fatty acids from C. volubile stem, indicating a phagocytic action which may be attributed to the synergetic effect of the identified fatty acids.
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Abbreviations
- ROS:
-
Reactive oxygen species
- MMP:
-
Matrix metalloproteinase
- KOH:
-
Potassium hydroxide
- FAME:
-
Fatty acid methyl esters
- MHS:
-
Modified Hank’s solution
- FADH:
-
Flavin adenine dinucleotide
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Acknowledgements
One of the authors, Ochuko L. Erukainure, is thankful to the Third World Academy of Science (TWAS) for ICCBS-TWAS fellowship at the H.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, Pakistan.
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All studies were carried out under the approval and guidelines of the biological ethical committee of the Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan, in accordance to the Declaration of Helsinki.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
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The authors declare that they have no conflict of interest.
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The authors are grateful for the laboratory services of late Dr. A. Shukralla prior to publication.
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Erukainure, O.L., Zaruwa, M.Z., Mesaik, A.M. et al. Suppression of phagocytic oxidative burst, cytotoxic effect, and computational prediction of oral toxicity of dietary fatty acids of Clerodendrum volubile stem. Comp Clin Pathol 26, 663–671 (2017). https://doi.org/10.1007/s00580-017-2438-y
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DOI: https://doi.org/10.1007/s00580-017-2438-y