Comparative Clinical Pathology

, Volume 26, Issue 2, pp 405–409 | Cite as

Simultaneous application of human amniotic membrane and Tachosil® in the repair of recto-vaginal fistula in an animal model

  • Seyed Vahid Hosseini
  • Mostaan Haghpanah Aski
  • Ahmed Mohammed Ali Hussein Al-Hurry
  • Abdul-Razzak Kalaf Hassan
  • Hajar Khazraei
  • Zahra Zabangirfard
  • Mozhdeh Zamani
Original Article


Patients with recto-vaginal fistula (RVF) suffer from distressing symptoms like the passage of stool and gas through the vagina. Although surgeons employed various techniques to address this disorder, however, current techniques are invasive and their success rate is variable. Considering the notable features of human amniotic membrane (HAM) as a bio-prosthesis and Tachosil® as a type of biologic mesh, the present study was designed to assess the effect of HAM in the presence of Tachosil® on the repair of RVF in a rabbit model. Twelve New Zealand rabbits weighing 2–2.5 kg with the age of 6–8 months were selected, and RVF was created surgically in all of them. They were randomly divided into three groups for repair with HAM, repair with HAM in the presence of Tachosil®, and standard repair, respectively. Histopathological features were evaluated using modified scoring system. Statistical analysis was performed by SPSS software version 18 (SPSS Inc., Chicago, IL, USA) using the Kruskal-Wallis test. Gross examination and microscopic evaluation were performed after 4 weeks. Fistula healing occurred in all rabbits and no abscess or infection was detected. Microscopic fistula healing was scored based on epithelialization, collagenization, inflammation, neovascularization, necrosis, and granulation tissue. Differences among these three groups were not statistically significant (p > 0.05). Our findings revealed that the major positive effect on RVF repair was related to HAM and healing was not improved in the presence of Tachosil®. Further studies with larger sample size would be helpful for more detailed assessment.


Human amniotic membrane Tachosil® Recto-vaginal fistula Wound healing 



This study was extracted from Dr. Haghpanah Aski dissertation (no. 7188). The authors would like to express their sincere gratitude to Shiraz University of Medical Sciences for financially supporting the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All applicable guidelines for the care and use of animals were duly followed and that approval was obtained from the Institutional Review Board (IRB) and Ethics Committee of Shiraz University of Medical Sciences.


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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Seyed Vahid Hosseini
    • 1
  • Mostaan Haghpanah Aski
    • 2
  • Ahmed Mohammed Ali Hussein Al-Hurry
    • 3
  • Abdul-Razzak Kalaf Hassan
    • 3
  • Hajar Khazraei
    • 1
  • Zahra Zabangirfard
    • 1
  • Mozhdeh Zamani
    • 1
  1. 1.Colorectal Research CenterShiraz University of Medical SciencesShirazIran
  2. 2.Department of Surgery, School of MedicineShiraz University of Medical SciencesShirazIran
  3. 3.Department of SurgeryKarbala University of Medical SciencesKarbalaIraq

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