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Effect of ionophore salinomycin on the antibiotic resistance in Clostridium difficile detected in mice

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Abstract

In the present study, an in vivo model was established to pursue the possible influence of ionophore, salinomycin, on the mobile resistance genes in Clostridium difficile. Fecal samples of 48 healthy adult mice were initially checked for the presence of C. difficile using a species-specific pair of primers. The animals were subsequently divided into the following experimental groups as follows salinomycin + tetracycline, tetracycline, salinomycin, and control (no treatment), for 10 days. Fecal sample was then collected from each group at days 0, 3, and 5 posttreatment, anaerobically enriched in the thioglycolate broth followed by sub-culturing onto blood agar plates. The growth curve and minimum inhibitory concentration (MIC) of groups were then recorded. A PCR assay was also conducted to amplify tet A and tet M genes corresponding to tetracycline. Results showed that salinomycin likely influenced the mobile resistance gene in gram-positive bacteria. Moreover, it may reduce the natural resistance of gram-positive bacteria and also inhibit the transfer of tetracycline mobile resistance gene, in vivo.

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Acknowledgments

The authors would like to thank the Research Council of Shiraz University and School of Veterinary Medicine, Shiraz University, for financial and technical support of this study (Grant No. 71-GR-VT-5).

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Authors and Affiliations

  1. Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    S Hosseinzadeh, E Mirsadeghi & M Sayyadi

  2. Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    H Rajaian

  3. Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, P.O. Box: 1731-71345, Shiraz, Iran

    S Nazifi

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  1. S Hosseinzadeh
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  2. E Mirsadeghi
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  3. H Rajaian
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Correspondence to S Nazifi.

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The authors declare that they have no conflicts of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Hosseinzadeh, S., Mirsadeghi, E., Rajaian, H. et al. Effect of ionophore salinomycin on the antibiotic resistance in Clostridium difficile detected in mice. Comp Clin Pathol 25, 1137–1141 (2016). https://doi.org/10.1007/s00580-016-2317-y

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