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Comparative Clinical Pathology

, Volume 25, Issue 4, pp 743–747 | Cite as

Polyphenolic contents and antioxidant activities of two medicinal plant species, Mentha piperita and Stevia rebaudiana, cultivated in Iran

  • Sharareh Najafian
  • Mojtaba Moradi
  • Masood SepehrimaneshEmail author
Original Article

Abstract

Mentha piperita (peppermint) and Stevia rebaudiana (stevia) are now widespread in cultivation worldwide. However, reports about the contents of these two plant species are scarce. To establish the polyphenolic contents and antioxidant activity of these plants by ferric ion-reducing activity (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods, local plants were harvested in flowering stage. Samples without any previous treatments were analyzed using high-performance liquid chromatography (HPLC) to determine the polyphenolic contents. Also, their antioxidant properties were determined using FRAP and DPPH methods. Six phenolic compounds including quercetin, p-coumaric acid, trans-ferulic acid, hesperidin, hesperetin, and rosmarinic acid were identified and quantified in peppermint. However, hesperidin, eugenol, coumarin, and vanillin were the most component in stevia. Peppermint showed better antioxidant properties than stevia in both FRAP and DPPH assessments. Our findings demonstrated that these two plants contain high polyphenolic compounds and are potent antioxidant species. Further studies on the therapeutic properties of these plants in animal and human models of diseases are recommended.

Keywords

Peppermint Stevia Polyphenols Antioxidant activity FRAP DPPH 

Notes

Acknowledgments

The authors would like to thank the superintendent of the Department of Natural Resources, Fars Research Center for Agriculture and Natural Resources, Dr. Vahid Rowshan, for his kindness and for providing the laboratory facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

Human and animal rights and informed consent

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Sharareh Najafian
    • 1
  • Mojtaba Moradi
    • 2
  • Masood Sepehrimanesh
    • 3
    Email author
  1. 1.Department of AgriculturePayame Noor UniversityTehranIran
  2. 2.Department of Horticultural Science, Shiraz BranchIslamic Azad UniversityShirazIran
  3. 3.Gastroenterohepatology Research CenterShiraz University of Medical SciencesShirazIran

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