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Changes in reduced glutathione, oxidized glutathione, and glutathione peroxidase in cats with naturally occurring chronic kidney disease

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Abstract

Chronic kidney disease (CKD) is one of the most common diseases in geriatric cats. The objective of this study was to determine oxidative stress in CKD cats by measuring changes in reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GPx), and reduced glutathione to oxidized glutathione (GSH/GSSG) ratio in cats with naturally occurring chronic kidney disease (CKD). Thirteen clinically normal client-owned aged-matched cats and 23 naturally occurring CKD cats were included. Completed blood count, blood urea nitrogen, creatinine, GSH, GPx, GSSG, and GSH/GSSG ratio were measured on the first day of diagnosis. The results showed that CKD cats had significantly lower GSH, GPx, and GSH/GSSG ratio levels (2.77 ± 0.27 mM, 2.32 ± 0.40 nmol/min and 148.26 ± 34.19) than the clinically normal client-owned age-matched cats (4.23 ± 0.67 mM, 6.68 ± 0.79 nmol/min and 312.64 ± 76.80). The GSSG in the CKD cats (35.20 ± 4.37 μM) was significantly higher than in the clinically normal client-owned age-matched cats (19.66 ± 2.75 μM). The results indicated that cats with naturally occurring CKD experience oxidative stress.

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Notes

  1. 1. SPSS version 17.0, SPSS Co, Chicago, Ill.

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Acknowledgments

This study was supported by grants from the Graduate School, Chulalongkorn University.

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Authors and Affiliations

  1. Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

    Kakanang Piyarungsri & Rosama Pusoonthornthum

Authors
  1. Kakanang Piyarungsri
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  2. Rosama Pusoonthornthum
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Correspondence to Rosama Pusoonthornthum.

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All applicable Chulalongkorn University Animal Care and Use Committee guidelines for the care and use of animals were followed.

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The authors declare that they have no conflict of interest.

Funding

This study was support by grant from the Graduate School, Chulalongkorn University.

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Piyarungsri, K., Pusoonthornthum, R. Changes in reduced glutathione, oxidized glutathione, and glutathione peroxidase in cats with naturally occurring chronic kidney disease. Comp Clin Pathol 25, 655–662 (2016). https://doi.org/10.1007/s00580-016-2248-7

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