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Blood biochemistry responses of ducks infected with a velogenic Newcastle disease virus

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Abstract

This study investigated the blood biochemistry responses of Pekin ducks (Anas platyrhynchos domesticus) experimentally infected with a velogenic Newcastle disease virus (NDV), KUDU 113 strain. One hundred ducklings were used for the study. The ducks were obtained at a week old and randomly divided into three groups. One group was vaccinated against NDV with La Sota vaccine at 3 weeks of age. The vaccinated and unvaccinated groups of 30 ducks each were subsequently challenged with the velogenic NDV after 6 weeks of brooding, while the control group of 40 ducks was not vaccinated and not challenged. Blood samples were randomly collected from five birds in each group, allowed for about 30 min to clot, centrifuged, and serum harvested. Blood biochemistry determinations were carried out at 3-day intervals from days 0 to 15 and day 21 post inoculation (PI). The results showed a significant increase in albumin on day 6 PI and total protein and globulin on day 9 PI and a significant increase in uric acid levels in the infected groups when compared with the unvaccinated uninfected controls from days 3 to 12 PI. The significant increase in uric acid level in both the vaccinated infected and unvaccinated infected groups in the absence of any overt clinical sign is noteworthy as uric acid determination can be a useful screening tool for detection of the velogenic NDV in apparently healthy ducks before they constitute a risk to in-contact poultry or spread to the environment.

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Authors and Affiliations

  1. Department of Veterinary Pathology, Michael Okpara University of Agriculture, Umudike, P. O. Box 3244, Umuahia, Abia State, Nigeria

    Christian O. Okorie-Kanu

  2. Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria

    Onyinye J. Okorie-Kanu

  3. Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria

    John O. A. Okoye

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  1. Christian O. Okorie-Kanu
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  2. Onyinye J. Okorie-Kanu
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  3. John O. A. Okoye
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Correspondence to Christian O. Okorie-Kanu.

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Okorie-Kanu, C.O., Okorie-Kanu, O.J. & Okoye, J.O.A. Blood biochemistry responses of ducks infected with a velogenic Newcastle disease virus. Comp Clin Pathol 25, 681–688 (2016). https://doi.org/10.1007/s00580-016-2247-8

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