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Antimicrobial activity of peptides derived from enzymatic hydrolysis of goat milk caseins

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Abstract

In this study, peptide fragments were produced from goat milk casein by proteolytic enzymes trypsin and ficin and combination of both enzymes, and their antimicrobial activity was investigated. After enzymatic treatments, antimicrobial activity against both Gram-positive and Gram-negative bacteria was enhanced. The hydrolysates were passed through ultrafiltration. The obtained hydrolysate by ficin (molecular weight (MW) <3 kDa) showed the highest antimicrobial activity and was selected for further purification by reversed-phase high-performance liquid chromatography (RP-HPLC). Twenty-seven peptide fractions were separated, and their antimicrobial activities were evaluated. The results showed that one of the fraction numbers (14) possessed the highest activity against Escherichia coli and Bacillus cereus. The results suggest that bioactive peptides obtained by digestion of goat milk caseins with ficin could be exploited to as natural antimicrobial agents in pharmaceutical industries

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Acknowledgments

This research was financially supported by grant number 94-GR-VT-11 from Shiraz University Research Council and grants from Natural Antimicrobial Center of Excellence, Iran, and Islamic Azad University, Science and Research Branch, Tehran, Iran.

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Authors and Affiliations

  1. Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mehrnaz Esmaeilpour, Mohammad Reza Ehsani & Ebrahim Hoseini

  2. Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Mahmoud Aminlari

  3. Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Shahram Shekarforoush

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  1. Mehrnaz Esmaeilpour
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  2. Mohammad Reza Ehsani
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  3. Mahmoud Aminlari
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  4. Shahram Shekarforoush
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  5. Ebrahim Hoseini
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Correspondence to Mahmoud Aminlari.

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Esmaeilpour, M., Ehsani, M.R., Aminlari, M. et al. Antimicrobial activity of peptides derived from enzymatic hydrolysis of goat milk caseins. Comp Clin Pathol 25, 599–605 (2016). https://doi.org/10.1007/s00580-016-2237-x

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  • DOI: https://doi.org/10.1007/s00580-016-2237-x

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