The influence of carob powder on lipid profile and histopathology of some organs in rats

Abstract

The present study aimed to investigate the effect of two levels of carob powder 10 and 20 % on lipid profile. Forty male Sprague–Dawley rats were divided into two groups. The first group was fed on a basal diet and acts as a control group. The second group was fed on a hyperlipidemic diet (a basal diet plus 10 % animal fat and 1 % cholesterol). After 6 weeks, the second group was divided into three even subgroups: subgroup 1 was fed on a hyperlipidemic diet, subgroup 2 was fed on a hyperlipidemic diet plus 10 % carob powder, and subgroup 3 was fed on a hyperlipidemic diet plus 20 % carob powder. At the end of the experimental period, serum samples were collected to measure total cholesterol (TC), triglyceride (TG), high-density lipoproteins (HDL), low-density lipoproteins (LDL), and very-low-density lipoproteins (VLDL). Heart and kidney samples were taken for histopathological examination. The obtained results revealed that feeding rats with 10 and 20 % carob powder improved lipid profile parameters and histopathological characteristics in the heart and kidney of experimental rats.

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References

  1. AHA (2005) Heart disease and stroke statistics, Ed. Dallas, TX: American Heart Association

  2. Ali MME, Abd El-Megeid AA, Abdo Mostafa RA (2012) Effect of some levels from ginseng, barley and carob on lipid profile and kidney functions of rats fed on high fructose diets. J Am Sci 8(10):152–162

    Google Scholar 

  3. Allian CC, Poon LS, Chan CSG, Richmond W (1974) Enzymatic colourimetric method of the determination of plasma total cholesterol. Clin Chem 20:470

    Google Scholar 

  4. Anon (1977) First report of the American Institute of Nutrition. J Nutrition 107:1340–1348

    Google Scholar 

  5. Anon (1980) Second report of the American Institute of Nutrition. J Nutrition 110:1726

    Google Scholar 

  6. Avallone R, Plessi M, Baraldi M, Monzani A (1997) Determination of chemical composition of carob (Ceratonia siliqua): protein, fat, carbohydrates and tannins. J Food Compost Anal 10:166–172

    CAS  Article  Google Scholar 

  7. Bancroft TD, Stevens A, Turner DR (1996) Theory and practice of histological technique, 4th edn. Churchill Livingston, New York

    Google Scholar 

  8. Brennan CS (2005) Dietary fiber, glycaemic response and diabetes. Mol Nutr Food Res 49:560–570

    Article  PubMed  Google Scholar 

  9. Burget L, Caton S, Bai Y, Spangler L, Gruendel S, Koebnick C, Bidlingmaier M (2007) Longterm effects of carob pulp preparations in insoluble fiber on metabolism, body weight and leptin levels in rats. Exp Clin Endocrinol Diabetes 115–124

  10. Deng R (2009) Food and food supplements with hypocholesterolemic effects. Recent patents on food. Nutr Agric 1:15–24

    CAS  Google Scholar 

  11. El-Manfaloty MM (2005) Effect of some Egyptian vegetables on the cholesterol in blood serum of white rats. MSc Home Economic, Nutrition and Food Science

  12. Fernandez ML, Webb D (2008) The LDL to HDL cholesterol ratio as a valuable tool to evaluate coronary heart disease risk. J Am Coll Nutr 27(1):1–5

    CAS  Article  PubMed  Google Scholar 

  13. Fossati SF, Prancipel R (1982) Triglycerides determination after enzymatic hydrolysis. Clin Chem 28:2077

    CAS  PubMed  Google Scholar 

  14. Friedewald WT, Levy RI, Fredrickson DS (1972) Determination of high density lipoprotein cholesterol by selective precipitation. Clin Chem 18:499–502

    CAS  PubMed  Google Scholar 

  15. Ilwy YME (2003) The effect of some kinds of seafood (fish) on blood lipid profile in rats. Dissertation, Faculty of Specific Education, Ain Shams University, Egypt

    Google Scholar 

  16. Klaus S, Pultz S, Thone-Reineke C, Wolfram S (2005) Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes 29:615–623

    CAS  Article  Google Scholar 

  17. Kumazawa S, Taniguchi M, Suzuki Y, Shimura M, Kwon MS, Nakayama T (2002) Antioxidant activity of polyphenols in carob pods. J Agric Food Chem 50(2):373–377

    CAS  Article  PubMed  Google Scholar 

  18. Makris DP, Kefalos P (2004) Carob pods (Ceratonia silique L.) as a source of polyphenolic antioxidants. Food Technol Biotechnol 42:105–108

    CAS  Google Scholar 

  19. Martínez-Rodríguez R, Navarro-Alarcón M, Rodríguez-Martínez C, Fonollá-Joya J (2013) Effects on the lipid profile in humans of a polyphenol- rich carob (Ceratonia Siliqua L.) extract in a dairy matrix like a functional food. Nutr Hop 28(6):2107–2114

    Google Scholar 

  20. Mathew V, Cannan CR, Miller VM (1997) Enhanced endothelin-mediated coronary vasoconstriction and attenuated basal nitric oxide activity in experimental hypercholesterolemia. Circulation 96:1930–1936

    CAS  Article  PubMed  Google Scholar 

  21. Meagher EA (2004) Addressing cardiovascular risk beyond low-density lipoprotein cholesterol: the high-density lipoprotein cholesterol story. Curr Cardiol Rep 6(6):457–463

    Article  PubMed  Google Scholar 

  22. Nicholls SJ, Tauzcu EM, Nissen SE (2007) Atherosclerosis regression: is low-density lipoprotein or high-density lipoprotein the answer? Curr Atheroscler Rep 9(4):266–273

    CAS  Article  PubMed  Google Scholar 

  23. Ruiz-Roso B, Quintela JC, de la Fuente E, Haya J, Pérez-Olleros L (2010) Insoluble carob fiber rich in polyphenols lowers total and LDL cholesterol in hypercholesterolemic subjects. Plant Foods Hum Nutr 65(1):50–56

    CAS  Article  PubMed  Google Scholar 

  24. Russell DF (1983) MSTAT Director crop and Soil sciences department, Michigan State University, version 2.10

  25. Steinberg D (2005) The pathogenesis of atherosclerosis. An interpretive history of the cholesterol controversy: part II: the early evidence linking hypercholesterolemia to coronary disease in humans. J Lipid Res 46(2):179–190

    CAS  Article  PubMed  Google Scholar 

  26. Thomas S, Rich MW (2007) Epidemiology, pathophysiology and prognosis of heart failure in the elderly. Heart sections of control rats had normal histoarchitecture. Clin Geriatr Med 23(1):1–10

    Article  PubMed  Google Scholar 

  27. Warnick GR, Benderson J, Albers JJ (1983) Dextran sulfate-Mg2+ precipitation procedure for quantitation of high density lipoprotein cholesterol. Cooper GR eds. Selected methods of clinical chemistry, 10:91–99 American Association for Clinical Chemistry Washington, DC

  28. Zunft HJ, Lüder W, Harde A, Haber B, Graubaum HJ, Gruenwald J (2001) Carob pulp preparation for treatment of hypercholesterolemia. Adv Ther 18(5):230–236

    CAS  Article  PubMed  Google Scholar 

  29. Zunft HJ, Lüder W, Harde A, Haber B, Graubaum HJ, Koebnick C, Grünwald J (2003) Carob pulp preparation rich in insoluble fiber lowers total and LDL cholesterol in hypercholesterolemic patients. Eur J Nutr 42(5):235–24

    CAS  Article  PubMed  Google Scholar 

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The authors declare that there is no conflict of interest.

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Correspondence to Khaled M. A. Hassanein.

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Hassanein, K.M.A., Youssef, M.K.E., Ali, H.M. et al. The influence of carob powder on lipid profile and histopathology of some organs in rats. Comp Clin Pathol 24, 1509–1513 (2015). https://doi.org/10.1007/s00580-015-2108-x

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Keywords

  • Carob powder
  • Rats
  • Lipid profile
  • Histopathology
  • Kidney
  • Heart