Abstract
Different studies have shown that oxidative stress has an important role in both types of diabetes mellitus. The aim of this study was to evaluate the effect of different doses of Carum carvi oil on antioxidant enzymes of streptozotocin-induced diabetic rats. Fifty-six Wistar male rats weighing between 250 ± 50 g were divided into seven equal groups. Diabetes was induced in the experimental rats with streptozotocin (60 mg kg−1 body weight). Rats in group I served as the normal control animals; group II served as the caraway control, whereas group III served as the diabetic control and group IV was the diabetic control which received sunflower oil. Groups V to VII were the test groups receiving various oral doses of caraway oil (5, 10, and 20 mg kg−1 body weight, respectively). The experiment lasted for a period of 30 days, and the following antioxidant enzymes were assayed: SOD, GSH-Px, CAT, and MDA. The results showed that rats receiving Carum carvi oil gained significantly more weight and had a lower level of blood glucose (P < 0.05) compared to the controls. In addition, activity of MDA was decreased, and the activities of SOD, GSH-Px, and CAT were significantly high (P < 0.05) in the test groups administered black caraway oil. It is concluded that caraway seed oil can be effective in reducing oxidative stress in diabetes mellitus.
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Asadollahi A, Sarir H, Omidi A, Montazer Torbati M (2014) Hepatoprotective potential of Prosopis farcta beans extracts against acetaminophen induced hepatotoxicity in Wistar rat. Int J Prev Med 5:1281–1285
Baynes JW, Thorpe SR (1996) The role of oxidative stress in diabetic complications. Curr Opin Endocrinol 3:277–284
Borcea V, Nourooz-Zadeh J, Wolff SP, Klevesath M, Hofmann M, Urich H, Wahl P, Ziegler R, Tritschler H, Halliwell B, Nawroth PP (1999) α-lipoic acid decreases oxidative stress even in diabetic patients with poor glycemic control and albuminuria. Free Radic Biol Med 26:1495–1500
Coskun O, Kanter M, Korkmaz A, Oter S (2005) Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas. Pharmacol Res 51:117–123
De Martino L, De Feo V, Fratianni F, Nazzaro F (2009) Chemistry, antioxidant, antibacterial and antifungal activities of volatile oils and their components. Nat Pro Commun 4:1741–1750
Eddouks M, Lemhadri A, Michel JB (2004) Caraway and caper: potential anti-hyperglycaemic plants in diabetic rats. J Ethnopharmacol 94:143–148
El-Missiry MA, El Gindy AM (2000) Amelioration of alloxan induced diabetes mellitus and oxidative stress in rats by oil of Eruca sativa seeds. Ann Nutr Metab 44:97–100
Ene AC, Bukbuk DN, Ogunmola OO (2006a) Effect of different doses of black caraway (Carum carvi L.) oil on the levels of serum creatinine in Alloxan induces diabetic rats. J Med Sci 6:701–703
Ene AC, Milala MA, Nwankwo EA (2006b) The effect of different doses of black caraway (Carum carvi L.) oil on the liver enzymes of alloxan-induced diabetic rats. J Med Sci 6:994–998
Ene AC, Nwankwo EA, Samdi LM (2007) Alloxan-induced diabetes in rats and the effects of black caraway (Carum carvi L.) oil on their body weight. Res J Med Sci 2:994–998
Friedman J, Peleg E, Kagan T, Shnizer S, Rosenthal T (2003) Oxidative stress in hypertensive, diabetic, and diabetic hypertensive rats. Am J Hypertens 16:1049–1052
Gajdosik A, Gajdosikova A, Stefek M, Navarova J, Hozova R (1999) Streptozotocin-induced experimental diabetes in male Wistar rats. Gen Physiol Biophys 18:54–62
Griesmacher A, Kindhauser M, Andert ES, Schreiner W, Toma C, Knoebl P, Pietschmann P, Prager R, Schnack C, Schernthaner G (1995) Enhanced serum levels of thiobarbituric acid reactive substances in diabetes mellitus. Am J Med 98:469–475
Haidari F, Sadjadi N, Jalali MT, Shahi M (2011) The effect of oral administration of Carum carvi on weight, serum glucose, and lipid profile in streptozotocin induced diabetic rats. Saudi Med J 32:695–700
Ihara Y, Toyokuni S, Uchida K, Odaka H, Tanaka T, Ikeda H (1999) Hyperglycemia causes oxidative stress in pancreatic beta-cells of GK rats a model of type 2 diabetes. Diabetes 48:927–932
Johri RK (2011) Cuminum cyminum and Carum carvi: an update. Pharmacogn Rev 6:63–72
Kamalakkannan N, Mainzen Prince PS (2006) Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-induced diabetic Wistar rats. Basic Clin Pharmacol Toxicol 98:97–103
Kaur K, Michael H, Arora S, Harkonen PL, Kumar S (2003) Studies on correlation of antimutagenic and antiproliferative activities of Juglans regia L. J Environ Pathol Toxicol Oncol 22:59–67
Kim EK, Choi EJ (2010) Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta Mol Basis Dis 1802:396–405
Korkina LG, Afanas’ev IB (1997) Antioxidant and chelating properties of flavonoids. Adv Pharmacol 38:151–163
Laight DW, Carrier MJ, Anggard EE (2000) Antioxidant, diabetes and endothelial dysfunction. Cardiovasc Res 47:457–464
Li ZG, Britton M, Sima AA, Dunbar JC (2004) Diabetes enhances apoptosis induced by cerebral ischemia. Life Sci 76:249–262
Lykkesfeldt J (2001) Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophotometry. Clin Chem 47: 1725–1727
Mazloomi SM, Shekarforoush SS, Sajedianfard J (2010) Effect of symbiotic yogurt on blood biochemical parameters in streptozotocin-Induced diabetic rats. J Vet Res 14:66–74
McCord JM, Fridovich I (1969) Superoxide dismutase, an enzymatic function for erythrocuprein. J Biol Chem 244:6049–6055
Miyake Y, Yamamoto K, Tsujihara N, Osawa T (1998) Protective effects of lemon flavonoids on oxidative stress in diabetic rats. J Lipid Res 33:689–695
Moore K, Roberts LJ (1998) Measurement of lipid peroxidation. Free Radic Res 28: 659-671
Moubarz G, Taha MM, Mahdy-Abdallah H (2014) Antioxidant effect of Carum carvi on the immune status of streptozotocin - induced diabetic rats infected with Staphylococcus aureus. World Appl Sci J 30:63–69
Moussa SA (2008) Oxidative stress in diabetes. Rom J Biophys 18:225–236
Najda A, Dyduch J, Brzozowski N (2008) Flavonoid content and antioxidant activity of caraway roots (Carum carvi L). Veg Crop Res Bull 68:127–133
Nuraliev IN, Avezov GA (1992) The efficacy of quercetin in alloxan diabetes. Eksp Klin Farmakol 55:42–44
Omidi A, Rahdari S, Hasanpoorfard M (2014) A preliminary study on antioxidant activities of saffron petal extracts in lambs. Vet Sci Dev 4:5161–5164
Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169
Pietta PG (2000) Flavonoids as antioxidants. J Nat Prod 63:1035–1042
Ramesh B, Pugalendi KV (2006) Antihyperglycemic effect of umbelliferone in streptozotocin-diabetic rats. J Med Food 9:562–566
Ranjbarian P, Sadeghian S, Shirazi M, Sarraf-Nejad A, Fazeli M, Amin G (2004) Antimicrobial properties of four plant essential oils and essences against H. pylori using disc diffusion and flow cytometry methods. Scientific J Med Uni Ham 33:42–47
Rodov V, Vinokur Y, Gogia N, Chkhikvishvili I (2010) Hydrophilic and lipophilic antioxidant capacities of Georgian spices for meat and their possible health implications. Georgian Med News 179:61–66
Rousselot DB (2004) The role of antioxidant micronutrients in the prevention of diabetic complications. Treat Endocrinol 3:41–52
Ruberto G, Baratta MT (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem 69:167–174
Ruhe RC, McDonald RB (2001) Use of antioxidant nutrients in the prevention and treatment of type 2 diabetes. Amer J Coll Nutr 20:363S–369S
Sadiq S, Nagi AH, Shahzad M, Zia A (2010) The reno-protective effect of aqueous extract of Carum carvi (black zeera) seeds in streptozotocin induced diabetic nephropathy in rodent. Saudi J Kidney Dis Transpl 21:1058–1065
Samojlik I, Lakic N, Mimica-Dukic N, Dakovic-Svajcer K, Bozin B (2010) Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and caraway (Carum carvi L.) (Apiaceae). J Agric Food Chem 58:8848–8853
Sanders RA, Rauscher FM, Watkins JB (2001) Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. J Biochem Mol Toxicol 15:143–149
Shirwaikar A, Rajendran K, DineshKumar C, Bodla R (2004) Antidiabetic activity of aqueous leaf extract of Annona squamosa in streptozotocin-nicotinamide type 2 diabetic rats. J Ethnopharmacol 91:171–175
Slaughter MR, O'Brien PJ (2000) Fully-automated spectrophotometric method for measurement of antioxidant activity of catalase.Clin Biochem 33(7):525–534
Sriplanga K, Adisakwattanab S, Rungsipipatc A, Yibchok-anuna S (2007) Effects of Orthosiphon stamineus aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats. J Ethnopharmacol 109:510–514
Stavic B (1994) Role of chemopreventers in human diet. Clin Biochem 27:319–332
Szkudelski T (2001) The Mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 50:536–546
Thippeswamya NB, Akhilender Naidub K, Achur Rajeshwara N (2013) Antioxidant and antibacterial properties of phenolic extract from Carum carvi L. J Pharmacy Res 7:352–357
Wolff SP (1993) Diabetes mellitus and free radical. Free radicals, transition metals and oxidative stress in the etiology of diabetes mellitus and complications. Br Med Bull 49:643–649
Yang H, Jin X, Kei Lam CW, Yan SK (2011) Oxidative stress and diabetes mellitus. Clin Chem Lab Med 49:1773–1782
Yu LL, Zhou KK, Parry J (2005) Antioxidant properties of cold pressed black caraway, carrot, cranberry, and hemp seed oils. Food Chem 91:723–729
Zafar M, Naqvi SN (2010) Effects of STZ-induced diabetes on the relative weights of kidney, liver and pancreas in Albino rats: a comparative study. Int J Morphol 28:135–142
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The authors would like to thank the Research Council of Shiraz University and School of Veterinary Medicine, Shiraz University for financial and technical support of this study (Grant No. 71-GR-VT-5).
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Erjaee, H., Rajaian, H., Nazifi, S. et al. The effect of caraway (Carum carvi L.) on the blood antioxidant enzymes and lipid peroxidation in streptozotocin-induced diabetic rats. Comp Clin Pathol 24, 1197–1203 (2015). https://doi.org/10.1007/s00580-014-2060-1
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DOI: https://doi.org/10.1007/s00580-014-2060-1