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Stereological evaluation of testes in busulfan-induced infertility of hamster

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Abstract

Cytotoxic medications can influence spermatogenesis at least temporarily and in some cases permanently. Busulfan is a cytotoxic bifunctional alkylating agent which inhibits cell division by sticking to DNA strands. This study aims to stereologically evaluate the testes in busulfan-induced infertility of azoospermic albino hamsters. Eighteen male adult albino hamsters were randomly divided into 3 equal groups. The first group received just one dose of busulfan (10 mg/kg, intraperitoneally, Busilvex®, France), the second group received two doses of busulfan with 21-days interval, and the control group left identically without any busulfan therapy. On day 35 postinjection, the testes of all animals were removed for histological evaluation and also for stereological indices. Lumen, cellular and total diameters, luminal, cellular and cross-sectional areas, number of tubules per unit area of testis, numerical density of the tubules, and spermatogenesis index in hamsters that received two doses of busulfan were significantly more than the hamsters receiving one dose of busulfan and the control group. Two doses of busulfan injected with 21-days interval could properly induce azoospermia in the experimental hamster model.

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References

  • Anjamrooz SH, Movahedin M, Mowla SJ, Bairanvand SP (2007) Assessment of morphological and functional changes in the mouse testis and epididymal sperms following busulfan treatment. Iran Biomed J 11:15–22

    CAS  PubMed  Google Scholar 

  • Bucci LR, Meistrich ML (1987) Effects of busulfan on murine spermatogenesis: cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations. Mutat Res Fund Mol Mech Mut 176:259–268

    Article  CAS  Google Scholar 

  • Chang CL-T, Fung H-P, Lin Y-F, Kuo C-Y, Chien C-W (2002) Indenopyridine hydrochloride induced testicular spermatogenesis failure with high seminal alkaline phosphatase levels in male dog. Biol Pharm Bull 25:1097–1100

    Article  CAS  PubMed  Google Scholar 

  • Choi Y-J, Ok D-W, Kwon D-N, Chung J-I, Kim H-C, Yeo S-M, Kim T, Seo H-G, Kim J-H (2004) Murine male germ cell apoptosis induced by busulfan treatment correlates with loss of c-kit-expression in a Fas/FasL-and p53-independent manner. FEBS Lett 575:41–51

    Article  CAS  PubMed  Google Scholar 

  • de Rooij DG, Vergouwen RP (1990) The estimation of damage to testicular cell lineages. Prog Clin Biol Res 372:467–480

    Google Scholar 

  • Dohle GR (2010) Male infertility in cancer patients: review of the literature. Int J Urol 17:327–331

    Article  PubMed  Google Scholar 

  • Gilliland KO, Freel CD, Lane CW, Fowler WC, Costello MJ (2001) Multilamellar bodies as potential scattering particles in human age-related nuclear cataracts. Mol Vis 7:120–130

    CAS  PubMed  Google Scholar 

  • Gundersen HJG (1977) Notes on the estimation of the numerical density of arbitrary profiles: the edge effect. J Microsc 111:219–223

    Article  Google Scholar 

  • Gundersen HJG, Jensen EB (1987) The efficiency of systematic sampling in stereology and its prediction. J Microsc 147:229–263

    Article  CAS  PubMed  Google Scholar 

  • Iwamoto T, Hiraku Y, Oikawa S, Mizutani H, Kojima M, Kawanishi S (2004) DNA intrastrand cross-link at the 5′-GA-3′ sequence formed by busulfan and its role in the cytotoxic effect. Cancer Sci 95:454–458

    Article  CAS  PubMed  Google Scholar 

  • Jiang FX (1998) Behaviour of spermatogonia following recovery from busulfan treatment in the rat. Anat Embryol (Berl) 198:53–61

    Article  CAS  Google Scholar 

  • Kramer MF, De Rooij DG (1969) The effect of three alkylating agents on the seminiferous epithelium of rodents. Virchows Archiv B 4:276–282

    Google Scholar 

  • Krause VW (1975) Teratogenic effect of busulfan on testis cells of the rat (postnatal development). Arzneimittelforschung 25:644

    CAS  PubMed  Google Scholar 

  • Krivoy N, Hoffer E, Lurie Y, Bentur Y, Rowe JM (2008) Busulfan use in hematopoietic stem cell transplantation: pharmacology, dose adjustment, safety and efficacy in adults and children. Curr Drug Saf 3:60–66

    Article  CAS  PubMed  Google Scholar 

  • Le Bourgeois A, Lestang E, Guillaume T, Delaunay J, Ayari S, Blin N, Clavert A, Tessoulin B, Dubruille V, Mahe B, Roland V, Gastinne T, Le Gouill S, Moreau P, Mohty M, Planche L, Chevallier P (2013) Prognostic impact of immune status and hematopoietic recovery before and after fludarabine, IV busulfan, and antithymocyte globulins (FB2 regimen) reduced-intensity conditioning regimen (RIC) allogeneic stem cell transplantation (allo-SCT). Eur J Haematol 90:177–186

    Article  PubMed  Google Scholar 

  • Nieto Y, Thall P, Valdez B, Andersson B, Popat U, Anderlini P, Shpall EJ, Bassett R, Alousi A, Hosing C, Kebriaei P, Qazilbash M, Frazier E, Gulbis A, Chancoco C, Bashir Q, Ciurea S, Khouri I, Parmar S, Shah N, Worth L, Rondon G, Champlin R, Jones RB (2012) High-dose infusional gemcitabine combined with busulfan and melphalan with autologous stem-cell transplantation in patients with refractory lymphoid malignancies. Biol Blood Marrow Transplant 18:1677–1686

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Panahi M, Keshavarz S, Rahmanifar F, Tamadon A, Mehrabani D, Karimaghai N, Sepehrimanesh M, Aqababa H (2015) Busulfan induced azoospermia: stereological evaluation of testes in rat. Vet Res Forum. In press

  • Suttorp M, Millot F (2010) Treatment of pediatric chronic myeloid leukemia in the year 2010: use of tyrosine kinase inhibitors and stem-cell transplantation. ASH Education Program Book 2010:368–376

  • Wallace WHB, Anderson RA, Irvine DS (2005) Fertility preservation for young patients with cancer: who is at risk and what can be offered? Lancet Oncol 6:209–218

    Article  PubMed  Google Scholar 

  • Wang DZ, Zhou XH, Yuan YL, Zheng XM (2010) Optimal dose of busulfan for depleting testicular germ cells of recipient mice before spermatogonial transplantation. Asian J Androl 12:263–270

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Whitsett JM, Noden PF, Cherry J, Lawton AD (1984) Effect of transitional photoperiods on testicular development and puberty in male deer mice (Peromyscus maniculatus). J Reprod Fertil 72:277–286

    Article  CAS  PubMed  Google Scholar 

  • Zhang Z, Shao S, Meistrich ML (2006) Irradiated mouse testes efficiently support spermatogenesis derived from donor germ cells of mice and rats. J Androl 27:365–375

    Article  PubMed  Google Scholar 

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Acknowledgments

The authors wish to thank the financial and scientific support of Shiraz University of Medical Sciences and Islamic Azad University.

Conflict of interest

There is no conflict of interest.

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Authors and Affiliations

  1. Stem Cells and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Mohadeseh Panahi, Negar Karimaghai, Amin Tamadon & Davood Mehrabani

  2. Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Farhad Rahmanifar

  3. Department of Physiology, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran

    Akbar Vahdati

  4. Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Omid Koohi-Hosseinabadi

  5. Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Masood Sepehrimanesh

Authors
  1. Mohadeseh Panahi
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  2. Negar Karimaghai
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  3. Farhad Rahmanifar
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  4. Amin Tamadon
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  5. Akbar Vahdati
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  6. Davood Mehrabani
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  7. Omid Koohi-Hosseinabadi
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  8. Masood Sepehrimanesh
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Corresponding authors

Correspondence to Akbar Vahdati or Davood Mehrabani.

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Panahi, M., Karimaghai, N., Rahmanifar, F. et al. Stereological evaluation of testes in busulfan-induced infertility of hamster. Comp Clin Pathol 24, 1051–1056 (2015). https://doi.org/10.1007/s00580-014-2029-0

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  • DOI: https://doi.org/10.1007/s00580-014-2029-0

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