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Hematological, serum biochemical, and immunological responses in common carp (Cyprinus carpio) exposed to phosalone

Comparative Clinical Pathology volume 24, pages 497–507 (2015)Cite this article

Abstract

This study was conducted to investigate the effects of sublethal phosalone concentrations on the hematological, serum biochemical, and immunological responses of carp (Cyprinus carpio). The fish were semi-statically exposed to low (0.15 mg/L), medium (0.3 mg/L), and high (0.6 mg/L) phosalone concentrations for 14 days. Results showed that the hematological variables red blood cell count (RBC, ×106/mm3), white blood cell count (×103/mm3), hematocrit (Hct, %), hemoglobin (Hb) concentration (g/dL), corpuscular volume mean (MCV, μm3), corpuscular Hb mean (MCH, pg), and mean corpuscular Hb concentration (MCHC, %) were significantly affected by phosalone concentrations. In addition, white blood cell types (neutrophil, lymphocyte, and monocyte) were also affected. Furthermore, serum proteins, metabolites, lipids, enzymes, and electrolytes were significantly altered by different phosalone concentrations. Nitroblue tetrazolium (NBT), lysozyme, and myeloperoxidase activities significantly altered treatment with phosalone concentrations. As a result, phosalone resulted in significant physiological and behavioral changes in C. carpio. These changes may potentially impair C. carpio’s survival capability in natural habitats or aquaculture.

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Acknowledgments

This study was supported by the Çanakkale Onsekiz Mart University Scientific Foundation (BAP) (Project No. 2010/80).

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Authors and Affiliations

  1. Department of Basic Science, Faculty of Marine Sciences and Technology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Hasan Kaya, Ekrem Şanver Çelik, Arınç Tulgar & Mehmet Akbulut

  2. Department of Aquaculture, Faculty of Marine Sciences and Technology, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey

    Sevdan Yılmaz

  3. Department of Biology, Faculty of Arts and Science, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey

    Neslihan Demir

Authors
  1. Hasan Kaya
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  2. Ekrem Şanver Çelik
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  3. Sevdan Yılmaz
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  4. Arınç Tulgar
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  5. Mehmet Akbulut
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Correspondence to Hasan Kaya.

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Kaya, H., Çelik, E.Ş., Yılmaz, S. et al. Hematological, serum biochemical, and immunological responses in common carp (Cyprinus carpio) exposed to phosalone. Comp Clin Pathol 24, 497–507 (2015). https://doi.org/10.1007/s00580-014-1930-x

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  • DOI: https://doi.org/10.1007/s00580-014-1930-x

Keywords

  • Phosalone
  • Hematology
  • Biochemistry
  • Immunology
  • Cyprinus carpio