Abstract
The use of mesenchymal stem cells (MSCs) in cell graft transplantation is advantageous; therefore, preservation of these cells is of utmost importance in repair therapies. To evaluate the viability ratio of the MSCs after cryopreservation, umbilical cord blood obtained from nine 46–48-week-old calves was purified for harvesting MSCs. The purified cells were frozen from the first to tenth passages and stored in liquid nitrogen. After thawing, the cell viability was assessed through trypan blue staining procedure. The highest viability (>80 %) ratio was observed with the cells derived from the first passage in 1 and 8 weeks after cryopreservation. However, the viability of cells was dependent on the passage used for cryopreservation. Results in this study demonstrated that MSCs obtained from bovine umbilical cord blood could successfully be frozen and stored in liquid nitrogen for 8 weeks without significant change in the characterization of the cells cryopreserved as regards their viability, growth ability, and differentiation potential.
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Acknowledgement
The authors thank Professor Robert Jackson and Professor Lucas De Hu for the help in histopathology and the staff of the Center for Stem Cell Biology and Tissue Engineering, the University of Queensland, Australia.
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Rastegar, A.M., Pahlavanzadeh, F., Vahdani, R. et al. The effect of cell passage on the viability of mesenchymal stem cells after cryopreservation. Comp Clin Pathol 24, 403–408 (2015). https://doi.org/10.1007/s00580-014-1915-9
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DOI: https://doi.org/10.1007/s00580-014-1915-9