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Hematological parameters and nuclear abnormalities in peripheral erythrocytes of Achirus lineatus (Pleuronectiformes: Achiridae)

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Abstract

Hematological parameter of demersal fish constitutes important measures of biological effects of seasonality and contaminants in sediments. We aimed to assess the hematological parameters and nuclear abnormalities, including micronuclei levels, in peripheral erythrocytes of flatfish Achirus lineatus collected in São Vicente Estuary in summer and winter. The number of lymphocytes was significantly higher in summer, whereas the number of neutrophils was significantly higher in winter. For other measured parameters, no significant differences were observed in spite of the levels of erythroblasts, leukocytes, thrombocytes, micronuclei and nuclear abnormality tended to be slightly higher in winter. Integrated analysis of data suggests that genotoxicity may be related to immunosuppression, although some types of leukocytes appear to act removing anomalous cells. Considering the contamination status of the Santos–São Vicente Estuarine system, the results provide an important contribution to knowledge of the hematological aspects of A.lineatus and its potential use as a bioindicator for monitoring estuarine sites.

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Acknowledgments

The authors gratefully acknowledge Mr. Ricardo for his assistance with the fish sampling. L.M. Buruaem (PhD grant 142002/2010-0) and D.M.S. Abessa (552299/2010-3) were sponsored by CNPq.

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Authors and Affiliations

  1. Universidade Paulista (UNIP), Campus Paraíso. R. Vergueiro, 1211, 01504001, São Paulo, SP, Brazil

    Lílian R. G. B. Prado, Cristiane Felix, Gabriela C. R. Turatti & Robson Seriani

  2. Campus Experimental do Litoral Paulista, Universidade Estadual Paulista (UNESP), Praça Infante Dom Henrique, s/n, 11330-900, São Vicente, SP, Brazil

    Denis M. S. Abessa

  3. Instituto de Ciências do Mar (LABOMAR-UFC), Universidade Federal do Ceará, Av. da Abolição, 3207, 60165-081, Fortaleza, CE, Brazil

    Lucas M. Buruaem

  4. Universidade Santa Cecília (UNISANTA), R. Oswaldo Cruz, 277, 11045-907, Santos, SP, Brazil

    Laís D. Abujamara

  5. Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191, 05508-900, São Paulo, SP, Brazil

    Aline A. Kirschbaum

  6. Instituto de Pesca, Av. Francisco Matarazzo, 455, 05001-000, São Paulo, SP, Brazil

    Maria J. T. Ranzani-Paiva

  7. Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil

    Aristides T. Correia & Robson Seriani

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  1. Lílian R. G. B. Prado
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  2. Cristiane Felix
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  3. Denis M. S. Abessa
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  6. Aline A. Kirschbaum
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Correspondence to Robson Seriani.

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Prado, L.R.G.B., Felix, C., Abessa, D.M.S. et al. Hematological parameters and nuclear abnormalities in peripheral erythrocytes of Achirus lineatus (Pleuronectiformes: Achiridae). Comp Clin Pathol 24, 169–175 (2015). https://doi.org/10.1007/s00580-014-1880-3

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