Skip to main content

Effect of Galega officinalis leaf powder and Trigonella foenum-graecum seed powder on blood glucose levels and weight gain in a diabetes mellitus rat model


Blood glucose levels and body weight were assessed to show the biological effects of Trigonella foenum-graecum and Galega officinalis in streptozotocin-induced diabetic rats. Seventy adult female rats were allocated randomly into seven equal independent groups, negative control (n = 10, neither diabetes mellitus (DM) induction nor treatment) and positive control (n = 10, DM induction with streptozotocin (STZ) but no treatment), and the remaining 50 were divided into five equal groups of ten per group, T. foenum-graecum or G. officinalis solutions (1.5 or 3 g/kg) and metformin. Diabetes mellitus was induced by intraperitoneal injection of a single dose of streptozotocin. Blood samples were obtained by tail cut at two time points, 4 days after DM induction and at the end on day 30, and analysed for glucose content, and body weight was recorded on days 0, 4 and 30. Our findings demonstrated that T. foenum-graecum seeds are more potent than G. officinalis leaves with regards to hypoglycaemic properties, but body weight-reducing properties were similar between these two plant species. These results indicated that these two plant species can be used as a herbal treatment for DM and weight gain.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2


  • Alarcon-Aguilara FJ, Roman-Ramos R, Perez-Gutierrez S, Aguilar Contreras A, Contreras-Weber CC, Flores-Saenz JL (1998) Study of the anti-hyperglycemic effect of plants used as antidiabetics. J Ethnopharmacol 61:101–110

    Article  CAS  PubMed  Google Scholar 

  • Bailey CJ (1992) Biguanides and NIDDM. Diabetes Care 15(6):755–772

    Article  CAS  PubMed  Google Scholar 

  • Bailey C, Day C (2004) Metformin: its botanical background. Pract Diabetes Int 21(3):115–117

    Article  Google Scholar 

  • Chan M, Zhao J, Brown PN, Khan IA (2010) Phytochemical study of Galega officinalis. Planta Med 76(05):P62

    Article  Google Scholar 

  • Erturk O (2010) Antibacterial and antifungal effects of alcoholic extracts of 41 medicinal plants growing in Turkey. Czech J Food Sci 28(1):53–60

    Google Scholar 

  • Gupta A, Gupta R, Lal B (2001) Effect of Trigonella foenum-graecum (fenugreek) seeds on glycaemic control and insulin resistance in type 2 diabetes mellitus: a double blind placebo controlled study. J Assoc Phys India 49:1057

    CAS  Google Scholar 

  • Hannan JMA, Ali L, Rokeya B, Khaleque J, Akhter M, Flatt P, Abdel-Wahab YHA (2007) Soluble dietary fibre fraction of Trigonella foenum-graecum (fenugreek) seed improves glucose homeostasis in animal models of type 1 and type 2 diabetes by delaying carbohydrate digestion and absorption, and enhancing insulin action. Br J Nutr 97:514–521

    Article  CAS  PubMed  Google Scholar 

  • Karakas FP, Yildirim A, Turker A (2012) Biological screening of various medicinal plant extracts for antibacterial and antitumor activities. Turk J Biol 36:641–652

    Google Scholar 

  • Khosla P, Gupta DD, Nagpal RK (1995) Effect of Trigonella foenum graecum (fenugreek) on blood glucose in normal and diabetic rats. Indian J Physiol Pharmacol 39:173–174

    CAS  PubMed  Google Scholar 

  • Palit P, Furman B, Gray A (1999) Novel weight‐reducing activity of Galega officinalis in mice. J Pharm Pharmacol 51(11):1313–1319

    Article  CAS  PubMed  Google Scholar 

  • Raju J, Gupta D, Rao AR, Yadava PK, Baquer NZ (2001) Trigonella foenum graecum (fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol Cell Biochem 224(1–2):45–51

    Article  CAS  PubMed  Google Scholar 

  • Shani J, Goldschmid A, Joseph B, Arhonson Z, Sulman FG (1974) Hypoglycemic effect of Trigonella foenum graecum and Lupinus termis (Leguminosae) seeds and their major alkaloids in alloxan diabetic and normal rats. Arch Int Pharmacodyn Ther 210:27–37

    CAS  Google Scholar 

  • Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 50(6):537–546

    CAS  PubMed  Google Scholar 

  • Tripathi UN, Chandra D (2010) Anti-hyperglycemic and anti-oxidative effect of aqueous extract of Momordica charantia pulp and Trigonella foenum graecum seed in alloxan-induced diabetic rats. Indian J Biochem Biophys 47:227–233

    CAS  PubMed  Google Scholar 

  • Yadav M, Lavania A, Tomar R, Prasad GB, Jain S, Yadav H (2010) Complementary and comparative study on hypoglycemic and antihyperglycemic activity of various extracts of Eugenia jambolana seed, Momordica charantia fruits, Gymnema sylvestre, and Trigonella foenum graecum seeds in rats. Appl Biochem Biotechnol 160:2388–2400

    Article  CAS  PubMed  Google Scholar 

  • Yang Y (2011) Metformin for cancer prevention. Front Med 5(2):115–117

    Article  PubMed  Google Scholar 

Download references


We are grateful to all the staff at the Laboratory Animal Centre of Medical University of Shiraz for their interest and enthusiasm in this research. The authors also acknowledge Miss Zahra Almasi and Mr. Omid Koohi for their help with the study.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Masood Sepehrimanesh.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shojaee, S.S., Vahdati, A., Assaei, R. et al. Effect of Galega officinalis leaf powder and Trigonella foenum-graecum seed powder on blood glucose levels and weight gain in a diabetes mellitus rat model. Comp Clin Pathol 24, 145–148 (2015).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: