Comparative Clinical Pathology

, Volume 23, Issue 5, pp 1583–1586 | Cite as

Survey on ovine toxoplasmosis by IFAT and double-tube nested-PCR in Tabriz, Iran

  • Ahmad Nematollahi
  • Razi allah Jafari
  • Parisa Shahbazi
  • Meimanat Khoshkerdar
  • Neda Zaboli
  • Maryam Nouruzi
Original Article


The aim of this study was to evaluate the presence of toxoplasmosis as a cause of ovine abortion in Tabriz by indirect flourescent antibody test (IFAT) and double-tube nested-polymerase chain reaction (PCR). From May 2011 to September 2012, 372 sera from sheep who had aborted, 120 aborted fetuses, and 60 placentae (180 samples) were collected in Tabriz, northwestern Iran. IgG antibodies specific to Toxoplasma gondii were used in sera by indirect immunofluorescence assay, whereas fetal and placenta samples were tested by double-tube nested-PCR. Specific IgG antibodies were detected in 129 (34.6 %) of the ewes who had aborted. From a total of 180 aborted fetal samples, 102 (56.6 %) were positive with 194 bp amplicon and, additionally, were confirmed positive with 93 bp amplicon. Results of this survey indicated that seroprevalence of T.gondii closely associated with ovine abortion in Tabriz. Preventive and hygienic procedures may be useful in controlling ovine toxoplasmosis in Tabriz.


Abortion IFAT Nested-PCR Sheep Toxoplasma gondii 



This research was supported by a research grant (D/27/3290-1) from the University of Tabriz.


  1. Asgari Q, Sarnevesht J, Kalantari M, Adnani-Sadat SJ, Motazedian MH, Sarkari B (2011) Molecular survey of toxoplasma infection in sheep and goat from Fars province, southern Iran. Trop Anim Health Prod 43:389–392PubMedCrossRefGoogle Scholar
  2. Burg JL, Grover CM, Pouletty P, Boothoyd JC (1989) Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol 27:1787–1792PubMedPubMedCentralGoogle Scholar
  3. Dubey JP (2009) Toxoplasmosis in sheep-the last 20 years. Vet Parasitol 163:1–14PubMedCrossRefGoogle Scholar
  4. Duncanson P, Terry RS, Smith JE, Hide G (2001) High levels of congenital transmission of Toxoplasma gondii in a commercial sheep flock. Int J Parasitol 31:1699–1703PubMedCrossRefGoogle Scholar
  5. Dworkin LL, Gibler TM, Van Gelder RN (2002) Real-time quantitative polymerase chain reaction diagnosis of infectious posterior uveitis. Arch Ophthalmol 120:1534–1539PubMedCrossRefGoogle Scholar
  6. Ghazaei C (2006) Serological survey of antibodies to Toxoplasma gondii. Afr J Health Sci 13:131–134PubMedGoogle Scholar
  7. Ghorbani M, Edrissian GH, Assad N (1993) Serological survey of toxoplasmosis in the northern part of Iran, using indirect fluorescent antibody technique. Trans R Soc Trop Med Hyg 72:369–371CrossRefGoogle Scholar
  8. Gutierreza J, O’Donovanb E, Williamsa A, Proctora C, Bradyc PX, Marquesa S, Worralla JE, Nallya M (2010) Detection and quantification of Toxoplasma gondii in ovine maternal and foetal tissues from experimentally infected pregnant ewes using real-time PCR. Vet Parasitol 172:8–15CrossRefGoogle Scholar
  9. Hamzavi Y, Mostafaie A, Nomanpour B (2007) Serological prevalence of toxoplasmosis in meat producing animals. Iran J Parasitol 2:7–11Google Scholar
  10. Hashemi-Fesharki R (1996) Seroprevalence of Toxoplasma gondii in cattle, sheep and goats in Iran. Vet Parasitol 61:1–3PubMedCrossRefGoogle Scholar
  11. Hoghooghi-Rad N, Afraa M (1993) Prevalence of toxoplasmosis in humans and domestic animals in Ahwaz, capital of Khoozestan province, south-west Iran. J Trop Med Hyg 96:163–168PubMedGoogle Scholar
  12. Homan WL, Vercammen M, De Braekeleer J, Verschuerer H (2000) Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol 30:69–75PubMedCrossRefGoogle Scholar
  13. Innes EA, Bartley PM, Buxton D, Katzer F (2009) Ovine toxoplasmosis. Parasitology 136:1884–1887CrossRefGoogle Scholar
  14. Keshavarz H, Mohebali M, Shahnazi V, Zarei Z (2007) Frequency of toxoplasma infection in livestock of Meshkin Shahr, by IFAT and its health importance. Tab Med J 2:115–118Google Scholar
  15. Khanmohammadi M (2011) Seroprevalence survey of Toxoplasma gondii antibodies among sheep in Tabriz district, northwest Iran. Ann Biol Res 6:484–488Google Scholar
  16. Kijlstra A, Jongert E (2009) Toxoplasma-safe meat: close to reality? Trends Parasitol 25:18–22PubMedCrossRefGoogle Scholar
  17. Masala G, Porcu R, Madau L, Tanda A, Ibba B, Satta G, Tola S (2003) Survey of ovine and caprine toxoplasmosis by IFAT and PCR assays in Sardinia, Italy. Vet Parasitol 117:15–21PubMedCrossRefGoogle Scholar
  18. Mason S, Quinnell RJ, Smith E (2010) Detection of Toxoplasma gondii in lambs via PCR screening and serological follow-up. Vet Parasitol 169:258–263PubMedCrossRefGoogle Scholar
  19. Payne S, Ellis JT (1996) Detection of Neospora caninum DNA by polymerase chain reaction. Int J Parasitol 26:347–351PubMedCrossRefGoogle Scholar
  20. Pereira-Bueno J, Quintanilla-Gozalo A, Pérez-Pérez, Alvarez-Garcia G, Collanets-Fernandez E, Ortega-Mora LM (2004) Evaluation ovine abortion associated with Toxoplasma gondii in Spain by different diagnostic techniques. Vet Parasitol 121:33–43PubMedCrossRefGoogle Scholar
  21. Raeghi S, Akaberi A, Sadeghi S (2011) Seroprevalence of Toxoplasma gondii in sheep, cattle and horses in Urmia north-west of Iran. Trop Anim Health Prod 43:389–392CrossRefGoogle Scholar
  22. Sharif M, Gholami S, Ziaei H, Daryani A, Laktarashi B, Ziapour SP, Rafiei A, Vahedi M (2007) Seroprevalence of Toxoplasma gondii in cattle, sheep and goats slaughtered for food in Mazandaran province, Iran, during 2005. Vet J 174:422–424PubMedCrossRefGoogle Scholar
  23. Tenter AM, Heckeroth AR, Weiss LM (2000) Toxoplasma gondii: from animals to humans. Int J Parasitol 30:1217–1258PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Ahmad Nematollahi
    • 1
  • Razi allah Jafari
    • 1
  • Parisa Shahbazi
    • 1
  • Meimanat Khoshkerdar
    • 1
  • Neda Zaboli
    • 1
  • Maryam Nouruzi
    • 1
  1. 1.Faculty of Veterinary MedicineUniversity of TabrizTabrizIran

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