Serum biochemical and hematological reference values are used to establish normality and to diagnose disease and physiological alterations. Up till now, there are no reference values for different blood biochemical and hematological variables in buffaloes, consequently the current study aimed to establish those reference values in non-pregnant lactating buffaloes. In total, 202 lactating buffaloes (5–10 years old) were examined at buffalo farms that belong to Assiut Governorate, Egypt. Seventy buffaloes did not meet the selection criteria and were excluded from study. The remaining buffaloes (N = 132) were included in the study. A total of 55 hematological and serum biochemical constituents were measured in blood of lactating buffaloes. Mean reference values, reference intervals (2.5 and 97.5 percentiles) and their 90 % confidence intervals, for the measured hematological and serum biochemical constituents were statistically calculated. It could be concluded that reference values for hematological and serum biochemical constituents in lactating buffaloes were estimated in this study, these values will be helpful in interpreting laboratory results, diagnosis of diseases, and checking the health status of non-pregnant lactating buffaloes.
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This work was supported by Basic and Applied Research Project (ID: 2947) from Science and Technology Development Fund (STDF), Ministry of Scientific Research, Egypt. The authors greatly appreciate the help of the members at the Department of Animal Health Care at the Veterinary Directorate for facilitating the examination of animals and collection of samples from buffalo farms.
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Abd Ellah, M.R., Hamed, M.I. & R. I., D. Serum biochemical and hematological reference values for lactating buffaloes. Comp Clin Pathol 23, 1179–1188 (2014). https://doi.org/10.1007/s00580-013-1760-2
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