Serum biochemical and hematological reference values for lactating buffaloes

Comparative Clinical Pathology volume 23, pages1179–1188(2014)Cite this article

Abstract

Serum biochemical and hematological reference values are used to establish normality and to diagnose disease and physiological alterations. Up till now, there are no reference values for different blood biochemical and hematological variables in buffaloes, consequently the current study aimed to establish those reference values in non-pregnant lactating buffaloes. In total, 202 lactating buffaloes (5–10 years old) were examined at buffalo farms that belong to Assiut Governorate, Egypt. Seventy buffaloes did not meet the selection criteria and were excluded from study. The remaining buffaloes (N = 132) were included in the study. A total of 55 hematological and serum biochemical constituents were measured in blood of lactating buffaloes. Mean reference values, reference intervals (2.5 and 97.5 percentiles) and their 90 % confidence intervals, for the measured hematological and serum biochemical constituents were statistically calculated. It could be concluded that reference values for hematological and serum biochemical constituents in lactating buffaloes were estimated in this study, these values will be helpful in interpreting laboratory results, diagnosis of diseases, and checking the health status of non-pregnant lactating buffaloes.

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References

  1. Abd Ellah MR (2011) Serum Metabolic Profile of Idiopathic Emaciated Buffaloes. J Anim Vet Adv 10:2456–2458

    CAS  Google Scholar 

  2. Ali A, Derar R, Hussein HA, Abd Ellah MR, Abdel-Razek AK (2011) Clinical, hematological, and biochemical findings of uterine torsion in buffaloes (Bubalus bubalis). Anim Reprod Sci 126:168–172

    PubMed  Article  CAS  Google Scholar 

  3. Anderson TW, Darling DA (1954) A Test of Goodness-of-Fit. J Am Stat Assoc 49:765–769

    Article  Google Scholar 

  4. Bailey SM, Sarmandal P, Grant JM (1989) A comparison of three methods for assessing inter-observer variation applied to measurement of the symphysis-fundal height. Br J Obstet Gynaecol 96:1266–1271

    PubMed  Article  CAS  Google Scholar 

  5. Barnouin J, Chassagne M, Chacornac JP (1997) Circulating monocyte and red cell counts as precalving predictors for retained placenta occurrence in dairy cows under field conditions in France. Epidemiol Sante Anim 31(32):05.21.1–05.21.3

    Google Scholar 

  6. Beechler BR, Jolles AE, Ezenwa VO (2009) Evaluation of hematologic values in free ranging African buffalo (Syncerus caffer). J Wildl Dis 45:57–66

    PubMed  Article  CAS  Google Scholar 

  7. Bertoni G, Bartocci S, Piccioli Cappelli, F, Amici A (1997) Blood metabolites and hormone changes in lactating buffaloes fed diets different for energy content and protein degradability. Proc. Fifth World Buffalo Congress. Caserta, Italia 961–965.

  8. Bertoni G, Lombardeli R, Piccioli Cappelli F, Bartocci S, Amici A (1994) Alcuni fattori che influenzano le condizioni endocrino-metaboliche della specie bufalina. Agr Ricerca 16:87–98

    Google Scholar 

  9. Bertoni G, Piccioli Cappelli F (1994) Indagine sull’allevamento delle bufale in provincia di Latina: influenza dell’alimentazione sulle condizioni metaboliche e produttive. Inf Agrario 18:19–33

    Google Scholar 

  10. Bertoni G, Piccioli-Cappelli F (1999) Guida all’interpretazione dei profili metabolici. Pubblicazione ASPA, ed. Università degli Studi di Perugia.

  11. Campanile G, Di Palo R, D’Angelo A (1997) Profilo metabolico nel bufalo. Bubalus Bubalis Suppl 4:236–249

    Google Scholar 

  12. Campanile G, Bonavoglia C, Perrucci GF, Di Palo R (1991) Caratteristiche ematochimiche in bufale appartenenti ad aziende con differente fertilità. Atti Fe Me S P Rum 1:93–98

    Google Scholar 

  13. Ciaramella P, Corona M, Ambrosio R, Consalvo F, Persechino A (2005) Haematological profile on non-lactating Mediterranean buffaloes (Bubalus bubalis) ranging in age from 24 months to 14 years. Res Vet Sci 79:77–80

    PubMed  Article  CAS  Google Scholar 

  14. Cockril WR (1977) The water buffalo. FAO, Rome

    Google Scholar 

  15. Cockril WR (1980) The ascendant water buffalo key domestic animal. World Anim Rev 33:2–13

    Google Scholar 

  16. Coles EH (1986) Veterinary Clinical Pathology, 4th edn. Saunders Comp, Philadelphia, London, Toronto

    Google Scholar 

  17. De Rosa C, Campanile G, Amante L, Banchelli L, Midea D, Zicarelli L (2001) Modificazioni endocrino-metaboliche nella fase di transizione e nei primi mesi di lattazione. Atti Primo Congresso Naz. Allevam. Bufalo, Salerno, Italia, pp 293–297

    Google Scholar 

  18. Fagiolo A, Lai O, Alfieri L, Nardon A, Cavallina R (2004) Environmental factors and different managements that influence metabolic, endocrine and immuno responses in water buffalo during lactation. Proc. Seventh World Buffalo Congress, Manila, Philippines, pp 24–26

    Google Scholar 

  19. FAO (1994) A manual for the primary animal health care worker. FAO Animal Health Manual Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  20. Farver TB (1997) Concepts of normality in clinical biochemistry. In: Kaneko JJ, Harvey JW, Bruss ML (eds) Clinical Biochemistry of Domestic Animals, 5th edn. Academic Pr, San Diego, pp 1–19

    Google Scholar 

  21. Geffré A, Concordet D, Braun JP, Trumel C (2011) Reference Value Advisor: a new freeware set of macroinstructions to calculate reference intervals with Microsoft Excel. Vet Clin Pathol 40:107–112

    PubMed  Article  Google Scholar 

  22. Geffré A, Friedrichs K, Harr K, Concordet D, Trumel C, Braun J-P (2009) Reference values: a review. Vet Clin Pathol 38:288–98

    PubMed  Article  Google Scholar 

  23. GOVS (2005) Technical veterinary report 2005. General Organization of Veterinary Service, Cairo, Egypt

    Google Scholar 

  24. Grasbeck R, Siest G, Wilding P, Williams GZ, Whitehead TP (1979) Provisional recommendation on the theory of reference values. Part 1. The concept of reference values. Clin Chem 25:1506–1508

    PubMed  CAS  Google Scholar 

  25. Grummer RR (1993) Etiology of lipid-related metabolic disorders in periparturient dairy cows. J Dairy Sci 76:3882–3896

    PubMed  Article  CAS  Google Scholar 

  26. Herdt TH, Goeders L, Liesman JS, Emery RS (1983) Test for estimation of bovine hepatic lipid content. J Am Vet Med Assoc 182:953–955

    PubMed  CAS  Google Scholar 

  27. Iverson SJ, Hamosh M, Bowen WD (1995) Lipoprotein lipase activity and its relationship to high milk fat transfer during lactation in grey seals. J Comp Physiol B 165:384–395

    PubMed  Article  CAS  Google Scholar 

  28. Jain NC, Vegad JL, Jain NK, Shrivastava AB (1982) Haematological studies on normal lactating Indian water buffaloes. Res Vet Sci 32:52–56

    PubMed  CAS  Google Scholar 

  29. Kaneko JJ (2008) Clinical biochemistry of domestic animals, 6th edn. Academic, San Diego

    Google Scholar 

  30. Khurshid KM, Khan TM, Basra MA, Zl C, Afzal M, Rashid J (1992) Serum cholesterol and total serum protein patterns in different age groups of lactating buffaloes. Pakistan J Agric Res 13:86–88

    Google Scholar 

  31. Klinkhoff AV, Bellamy N, Bombardier C, Carette S, Chalmers A, Esdaile JM, Goldschmidt C, Tugwell P, Smythe HA, Buchanan WW (1988) An experiment in reducing interobserver variability of the examination for joint tenderness. J Rheumatol 15:492–494

    PubMed  CAS  Google Scholar 

  32. Kulkarni BA, Talvelkar BA, Kaushik RV, Gokani SS, Patankar DD, Kulkarni BS (1984) Studies on serum biochemical constituents in lactating and dry Indian buffaloes. Indian Vet J 61:564–568

    CAS  Google Scholar 

  33. Latimer KS, Prasse KW, Mahaffey EA (2003) Proteins, Lipids, and Carbohydrates. In Veterinary Laboratory Medicine: Clinical Pathology, 4th ed. Ames, Iowa State University Press. pp. 162–192

  34. LeBlanc SJ, Leslie KE, Duffield TF (2005) Metabolic predictors of displaced abomasum in dairy cattle. J Dairy Sci 88:159–170

    PubMed  Article  CAS  Google Scholar 

  35. LeBlanc SJ, Lissemore KD, Kelton DF, Duffield TF, Leslie KE (2006) Major advances in disease prevention in dairy cattle. J Dairy Sci 89:1267–1279

    PubMed  Article  CAS  Google Scholar 

  36. Lombarts AJ, Koevoet AL, Leijnse B (1986) Basic principles and problems of haemocytometry. Ann Clin Biochem 23:390

    PubMed  Article  Google Scholar 

  37. Lumsden JH, Jacobs RM (1989) Clinical chemistry. in-clinic analysis, quality control, reference values, and system selection. Vet Clin North Am Small Anim Prac 19:875–897

    CAS  Google Scholar 

  38. Lumsden JH, Mullen K (1978) On establishing reference values. Can J Comp Med 42:293–301

    PubMed  CAS  PubMed Central  Google Scholar 

  39. Monteiro BM, Yasuoka MM, Pogliani FC, Ayres H, Viana RB, Birgel EH (2012) Lipid and glucose profiles of dairy buffaloes during lactation and dry period. Rev Cienc Agrar 55:33–39

    Google Scholar 

  40. Montemurro N, Pacelli C, Borghese A (1997) Blood metabolites change in milking buffalo cows. Bubalus Bubalis 3:69–78

    Google Scholar 

  41. Nawaz M, Siddique RH (1974) Normal pH of blood, total serum protein and cholesterol in serum of female buffaloes. Veterinarnome­ditsinski Nauki 26(3):211–214

    CAS  Google Scholar 

  42. Pattinson RC, Theron GB (1989) Inter-observer variation in symphysis-fundus measurement. S Afr Med J 76:621–622

    PubMed  CAS  Google Scholar 

  43. PetitClerc C, Solberg HE (1987) Approved recommendation on the theory of reference values. Part 2. Selection of individuals for the production of reference values. Clin Chem Acta 170:S1–S11

    Article  Google Scholar 

  44. Pizzuti GP, Salvatori GC (1993) Some blood parameters of water buffalo in different physiological conditions. Boll Soc Italian Sper 69:649–654

    CAS  Google Scholar 

  45. Pritchard JC, Burn CC, Barr ARS, Whay HR (2009) Haematological and serum biochemical reference values for apparently healthy working horses in Pakistan. Res Vet Sci 87:389–395

    PubMed  Article  CAS  Google Scholar 

  46. Radostits OM, Gay CC, Hinchcliff KW, Constable PD (2006) Veterinary Medicine: A Text Book of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 10th Edition, Bailliere Tindall London Philadelphia Sydney Tokyo Toronto. pp. 12–13.

  47. Ramkrishna KV (1991) Comparative studies on certain biochemical constituents of lactating and dry Murrha buffaloes. Livestock Advisor XIV:16–19

    Google Scholar 

  48. Reed AH, Henry RJ, Mason WB (1971) Influence of Statistical Method Used on the Resulting Estimate of Normal Range. Clin Chem 17:275–284

    PubMed  CAS  Google Scholar 

  49. Saleh MA, Rateb HZ, Misk NA (2008) Comparison of blood serum proteins in water buffaloes with traumatic reticuloperitonitis and sequellae. Res Vet Sci 85:208–213

    PubMed  Article  CAS  Google Scholar 

  50. Satriani A, Piccioli-Cappelli F, Palimeno F, Lambardelli R, Ferrara L, Bertoni G (2001) Fattori ambientali causa di variazioni endocrino-metaboliche nella bufala da latte. Atti I Congresso Naz. Allevam. Bufalo. Salerno, Italia, 3 5 Ott., pp 285 288.

  51. Smith BI, Risco CA (2005) Management of periparturient disorders in dairy cattle. Vet Clin North Am Food Anim Pract 21:503–521

    PubMed  Article  Google Scholar 

  52. Solberg HE (1987) Provisional recommendation on the theory of reference values. Part 5: statistical treatment of selected reference values. Determination of reference limits. J Clin Chem Clin Biochem 25:645–656

    CAS  Google Scholar 

  53. Solberg HE (1999) Establishment and use of reference values. In: Burtis CA, Ashwood ER, editors. Tietz Textbook of Clinical Chemistry. 3rd ed. New Dehli, India: Harcourt Brace; Asia Sauders. pp. 336–354.

  54. Soulsby EJL (1982) Helminths, arthropods and protozoa of domesticated animals (7th ed). ELBS, London. pp, 809

    Google Scholar 

  55. Tajik J, Nazifi S (2011) Serum Concentrations of Lipids and Lipoproteins and Their Correlations together and with Thyroid Hormones in Iranian Water Buffalo (Bulbalus bulbalis) Asian Journal of Animal Sciences 5: 196–201

    Google Scholar 

  56. Tajik J, Nazifi S, Heidari M, Babazadeh M (2012) Evaluation of Serum Proteins in Water Buffaloes (Bubalus bubalis) with Abomasal Ulcer. Asian J An Vet Adv 7:277–282

    Article  CAS  Google Scholar 

  57. Tajik J, Nazifi S, Izadneshan M, Naghib SM (2010) Evaluation of Trace Elements Serum Concentrations and Their Correlation Together, and with Thyroid Hormones in Water Buffalo (Bulbalus bulbalis). Aust J Basic Appl Sci 4(8):3955–3958

    CAS  Google Scholar 

  58. Terzano GM, Tripaldi C, Allegrini S, Pasqui E, Roncoroni C, Satà MC, Terramoccia S (2000): Benessere e riproduzione: approccio sperimentale sulla specie bufalina. Proc. Workshop Stato di benessere ed efficienza riproduttiva negli animali di interesse zootecnico. Viterbo, Italia 147–153.

  59. Theodossi A, Knill-Jones RP, Skene A, Lindberg G, Bjerregaard B, Holst-Christensen J, Williams R (1981) Inter-observer variation of symptoms and signs in jaundice. Liver 1:21–32

    PubMed  Article  CAS  Google Scholar 

  60. Tripathi PM, Ingole SD, Deshmukh BT, Nagvekar AS, Bharucha SV (2010) Serum lipid profile during lactation in buffalo. Indian J An Sci 80:217–219

    CAS  Google Scholar 

  61. Underwood EJ (1981) The Mineral Nutrition of Livestock. Commonwealth Agricultural Bureau, London, UK

    Google Scholar 

  62. Webster CC, Wilson PN (1980) Agriculture in the tropics, second ed. Longman (ELBS). London, UK, pp 390–400

    Google Scholar 

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Acknowledgments

This work was supported by Basic and Applied Research Project (ID: 2947) from Science and Technology Development Fund (STDF), Ministry of Scientific Research, Egypt. The authors greatly appreciate the help of the members at the Department of Animal Health Care at the Veterinary Directorate for facilitating the examination of animals and collection of samples from buffalo farms.

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Affiliations

  1. Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt

    M. R. Abd Ellah

  2. Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt

    Maha I. Hamed

  3. Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt

    Derar R. I.

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  1. M. R. Abd Ellah
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  2. Maha I. Hamed
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  3. Derar R. I.
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Correspondence to M. R. Abd Ellah.

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Abd Ellah, M.R., Hamed, M.I. & R. I., D. Serum biochemical and hematological reference values for lactating buffaloes. Comp Clin Pathol 23, 1179–1188 (2014). https://doi.org/10.1007/s00580-013-1760-2

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Keywords

  • Serum
  • Hematology
  • Buffalo
  • Lactating
  • Reference values