Comparative Clinical Pathology

, Volume 23, Issue 4, pp 1117–1123 | Cite as

Evaluation of the erythroplastid component of avian blood

  • P. ClarkEmail author
  • S. R. Raidal
Original Article


Avian erythrocytes are typically nucleated; however, a small proportion of anucleated erythrocytes, termed erythroplastids, may be encountered in the peripheral blood of birds. The blood films of 77 birds, encompassing 53 species and 15 orders, were assessed to characterise the erythroplastid component. Erythroplastids were recognised in the blood of 30 birds, representing 24 species and 10 orders with the greatest concentration of 23.52 × 109/L. This study documents the presence of erythroplastids in the blood of a wide range of avian species and describes the characteristics of erythroplastids and their relationship to the erythrocyte components of blood.


Anucleate RBC Denucleated RBC Enucleate RBC Erythrocyte Erythroplastid Polychromatophilic erythrocyte Rubricyte 



We thank Dr Martin Copland for his generous provision of the archive of blood films and haematological data and Mr Kevin Matschoss for the original processing of the samples.


  1. Bradley LW, Threlfall W (1974) Blood cell indices of five species of auk (Alcidae) from Newfoundland, Canada. J Zool 174:377–385CrossRefGoogle Scholar
  2. Clark P, Boardman WSJ, Raidal SR (2009) Atlas of clinical avian hematology. Wiley-Blackwell, OxfordGoogle Scholar
  3. Clark P, Hume A, Raidal SR (2013) Erythroplastidcytosis in a Major Mitchell cockatoo. Comp Clin Pathol. doi: 10.1007/s00580-013-1711-y Google Scholar
  4. Devyatkin AA, Revin VV, Yudanov MA, Kozlova OV, Samuilov VD (2006) Effect of hydrogen peroxide on ejection of cell nucleus from pigeon erythrocytes and state of membrane lipids. Bull Exp Biol Med 141:225–228CrossRefGoogle Scholar
  5. Emmel VE (1924) Studies of the non-nucleated elements of the blood. Am J Anat 33:347–405CrossRefGoogle Scholar
  6. Fudge AM (2000) Laboratory medicine: avian and exotic pets. WB Saunders Co., PhiladelphiaGoogle Scholar
  7. Gayathri KL, Hedge SN (1994) Sexual differences in blood values of the pigeon, Columba livia. Comp Biochem Physiol 109B:219–224Google Scholar
  8. Hajduk P, Copland MD, Schultz DA (1992) Effects of capture on hematological values and plasma cortisol levels of free-range koalas (Phascolarctos cinereus). J Wildl Dis 28:502–506PubMedCrossRefGoogle Scholar
  9. Hawkey CM (1991) The value of comparative haematological studies. Comp Haematol Int 1:1–9CrossRefGoogle Scholar
  10. Hawkey CM, Dennett TB (1989) A colour atlas of comparative veterinary haematology. Wolfe Publishing Ltd, London. ISBN 0-7234-0930-7Google Scholar
  11. Hawkey CM, Samour HJ (1988) The value of clinical haematology in exotic birds. In: Animals E (ed) Jacobson ER, Kollias GV. Churchill Livingstone, New York, pp 109–141Google Scholar
  12. Hawkey C, Samour JH, Ashton DG, Hart MG, Cindery RN, Finch JM, Jones DM (1983) Normal and clinical haematology of captive cranes (Gruiformes). Avian Pathol 12:73–84PubMedCrossRefGoogle Scholar
  13. Hiraga T, Ohyama K, Hashigaya A, Ishikawa T, Muramoto W, Kitagawa H, Mizuno N, Teraoka H (2008) Lead exposure induces pycnosis and enucleation of peripheral erythrocytes in the domestic fowl. Vet J 178:109–114PubMedCrossRefGoogle Scholar
  14. Howlett JC, Samour JH, D'Aloia MA, Bailey TA, Naldo J (1995) Normal haematology of captive adult kori bustards (Ardeotis kori). Comp Haematol Int 5:102–105CrossRefGoogle Scholar
  15. Johns JL, Shooshtari MP, Christopher MM (2008) Development of a technique for quantification of reticulocytes and assessment of erythrocyte regenerative capacity in birds. Am J Vet Res 69:1067–1072PubMedCrossRefGoogle Scholar
  16. Johnston MS, Son TT, Rosenthal KL (2007) Immune-mediated hemolytic anemia in an eclectus parrot. J Am Vet Med Assoc 230:1028–1031PubMedCrossRefGoogle Scholar
  17. Keerthivasan G, Small S, Liu H, Wickrema A, Crispino JD (2010) Vesicle trafficking plays a novel role in erythroblast enucleation. Blood 116:3331–3340PubMedCentralPubMedCrossRefGoogle Scholar
  18. Keerthivasan G, Wickrema A, Crispino JD (2011) Erythroblast enucleation. Stem Cells Int. doi: 10.4061/2011/139851, 139851. Published online 2011 October 5PubMedCentralPubMedGoogle Scholar
  19. Lavin S, Cuenca R, Marco I, Velarde R, Viñas L (1992) Hematology and blood chemistry of the marsh harrier (Circus aeruginosus). Comp Biochem Physiol 103A:493–495CrossRefGoogle Scholar
  20. Lucas AM, Jamroz C (1961) Atlas of avian hematology. Agriculture monograph 25. United States Department of Agriculture, WashingtonGoogle Scholar
  21. Migliaccio AR (2010) Erythroblast enucleation. Haematologica 12:1985–1988CrossRefGoogle Scholar
  22. Mueller RL, Gregory TR, Gregory SM, Hsieh A, Boore JL (2008) Genome size, cell size, and the evolution of enucleated erythrocytes in attenuate salamanders. Zoology 111:218–230PubMedCentralPubMedCrossRefGoogle Scholar
  23. Narkkong NA, Aengwanich W, Tanomthong A (2011) Morphology and morphometrics of hematological cells from eastern sarus crane, Grus antigone sharpii. Comp Clin Pathol 20:299–304CrossRefGoogle Scholar
  24. Palis J (2012) Losing a “nucleus” to gain a cytoplasm. Blood 119:5948–5949PubMedCrossRefGoogle Scholar
  25. Polo FJ, Peinado VI, Viscor G, Palomeque J (1998) Hematologic and plasma chemistry values in captive psittacine birds. Avian Dis 42:523–535PubMedCrossRefGoogle Scholar
  26. Samour JH, Naldo JL, John SK (2005) Normal haematological values in gyrfalcons (Falco rusticolus). Vet Rec 157:844–847PubMedGoogle Scholar
  27. Samour J, Naldo J, Rahman H, Sakkir M (2010) Hematologic and plasma biochemical reference values in Indian peafowl (Pavo cristatus). J Avian Med Surg 24:99–106PubMedCrossRefGoogle Scholar
  28. Wingstrand KG (1956) Non-nucleated erythrocytes in a teleostean fish Maurolicus mülleri (Gmelin). Z Zellforsch Mikrosk Anat 45:195–200PubMedGoogle Scholar

Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  1. 1.School of Biomedical SciencesCurtin UniversityBentleyAustralia
  2. 2.School of Agricultural and Veterinary SciencesCharles Sturt UniversityWagga WaggaAustralia

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