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Restorative effect of Dendrophthoe falcata (L.f.) Ettingsh on lipids, lipoproteins, and lipid-metabolizing enzymes in DMBA-induced mammary gland carcinogenesis in Wistar female rats

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Abstract

Lipid, lipoproteins, and lipid-metabolizing enzymes are associated with breast cancer risk. In this study, the potential of hydroalcoholic extract (HEDF) of Dendrophthoe falcata (L.f) Ettingsh (Loranthaceae) for the management of lipid metabolism on 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma was investigated in Wistar female rats. Thirty female rats (55 days of age) were divided into five groups: control, DMBA (25 mg in 0.5 ml of olive oil by air pouch technique), DMBA + HEDF (250, 475, and 950 mg/kg). After 90 days of induction, HEDF was administered for 28 days by gastric intubations. The levels of lipids, lipid-metabolizing enzymes, and lipoproteins were analyzed in the plasma and liver of both control and experimental animals. Rats treated with DMBA showed an increase (p < 0.05) in levels of phospholipids, triglycerides, free fatty acids, total cholesterol, and free cholesterol and a decrease (p < 0.01) in levels of ester cholesterol in the plasma and liver. The levels of very low-density lipoprotein and low-density lipoprotein increased (p < 0.01), while the levels of high-density lipoproteins decreased (p < 0.001) in plasma. Moreover, there was a significant increase (p < 0.001) in activities of total lipase, cholesterol ester hydrolase, cholesterol ester synthase but with a sharp decrease (p < 0.01) in lecithin cholesterol-acyl transferase and lipoprotein lipase in animals with mammary cancer. HEDF treatment caused the activity of these alterations in biochemical parameters to return to almost normal control levels. Also, histopathological analysis of the breast tissue was analyzed by hematoxylin and eosin staining, and results revealed the cytoprotective role of HEDF against mammary carcinogenesis. The effects of HEDF were found to be dose dependent in nature.

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Acknowledgments

The authors are indebted to Birla Institute of Technology (BIT), Mesra for financial assistance and for providing the necessary facilities to conduct the research. The authors are also thankful to the Government of Jharkhand for its continuous support for natural product research.

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Authors and Affiliations

  1. Anticancer Research Group, Division of Pharmacology, Birla Institute of Technology (BIT), Mesra, Ranchi, 835215, Jharkhand, India

    Shakti Prasad Pattanayak & P. Mitra Mazumder

  2. Government Pharmacy Institute, Government of Jharkhand, Bariatu, Ranchi, 834009, Jharkhand, India

    Priyashree Sunita

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  1. Shakti Prasad Pattanayak
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Correspondence to Shakti Prasad Pattanayak.

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Pattanayak, S.P., Sunita, P. & Mazumder, P.M. Restorative effect of Dendrophthoe falcata (L.f.) Ettingsh on lipids, lipoproteins, and lipid-metabolizing enzymes in DMBA-induced mammary gland carcinogenesis in Wistar female rats. Comp Clin Pathol 23, 1013–1022 (2014). https://doi.org/10.1007/s00580-013-1736-2

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