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Effect of sperm pH and density on fertilization success in Persian sturgeon Acipenser persicus (Borodin, 1897)

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Abstract

The objectives of the present study were to determine the relationships between sperm characteristics (density and pH) and fertilization capacity (hatching rate) for Persian sturgeon during their reproductive season. Eleven males were used in this study. The luteinizing hormone releasing hormone analog was used to induce spermiation following which semen samples were stripped. Statistical analysis revealed positive correlations between hatching rate and sperm pH (P < 0.05, r = 0.711) and between sperm pH and fertilization rate (P < 0.05, r = 0.633). Highly negative relationships were found between sperm density and hatching rate (P > 0.01, r = −0.745). We suggest that understanding these correlations is critical in the evaluation of sperm quality and for the composition of media (extender) to be used in the dilution of semen to improve sperm motility/fertility parameters of Persian sturgeon.

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References

  • Aas GH, Refstie T, Gjerde B (1991) Evaluation of milt quality of Atlantic salmon. Aquaculture 95:125–132

    Article  Google Scholar 

  • Agarwal NK (2005) Developing cryopreservation protocols for the spermatozoa of snow trout and other endangered coldwater species. [Final report.] ICARScheme, 105

  • Alavi SMH, Cosson J, Karami M, Amiri BM, Akhoundzadeh MA (2004) Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality. Reproduction 128:819–828

    Article  CAS  PubMed  Google Scholar 

  • Alavi SMH, Cosson J (2005) Sperm motility in fishes: (I) effects of pH and temperature. Cell Biol Int 29:101–110

    Article  PubMed  Google Scholar 

  • Alavi SMH, Cosson J, Kazemi R (2006) Semen characteristics in Acipenser persicus in relation to sequential stripping. Appl Ichthyol 22:400–405

    Article  Google Scholar 

  • Alavi SMH, Linhart O, Coward K, Rodina M (2008) Fish spermatology. In: Alavi SMH, Cosson J, Coward K, Rafiee G (eds) Implications for aquaculture management fish spermatology. Alpha Science Pub, Oxford, pp 397–460

    Google Scholar 

  • Azari Takami G (1999) New data on propagation and source of Beluga (Huso huso) Broodstock in Iran. Appl Ichthyol 15(4–5):I-352

    Google Scholar 

  • Baradaran Noveiri S, Alipour A, Pourkazemi M (2006) Sperm morphometry, density and spermatocrit study in Persian sturgeon (Acipenser persicus). Appl Ichthyol 22(1):380–383

    Article  Google Scholar 

  • Bencic DC, Cloud JG, Ingermann RL (2000) Carbon dioxide reversibly inhibits sperm motility and fertilizing ability in steelhead (Oncorhynchus mykiss). Fish Physiol Biochem 23:275–281

    Article  CAS  Google Scholar 

  • Billard R (1983) Effects of ceolomic and seminal fluids and various saline diluents on the fertilizing ability of spermatozoa in the Rainbow trout, Salmo gairdneri. J Reprod Fertil 68:77–84

    Article  CAS  PubMed  Google Scholar 

  • Billard R, Cosson J, Crim LW, Suquet M (1995) Broodstock management and egg and larval quality. In: Bromage NR, Roberts RJ (eds) Sperm physiology and quality. Blackwell Science, Oxford, pp 25–52

    Google Scholar 

  • Bromage N R, Cumaranataunga RC (1988) Egg production in the rainbow trout In: Muir JF, Robert RJ (eds) recent advances in Aquaculture, pp 63-139

  • Ciereszko A (2008) Chemical composition of seminal plasma and its physiological relationship with sperm motility, fertilizing capacity and cryopreservation success in fish. In: Alavi SMH, Cosson J, Coward K, Rafiee G (eds) Fish spermatology. Alpha Science Pub, Oxford, pp 215–240

    Google Scholar 

  • Ciereszko A, Dietrich G, Dietrich MA, Nynca J, Kuzminski H, Dobosz S, Grudniewska J (2010) Effects of pH on sperm motility in several Salmoniformes species: Oncorhynchus mykiss, Salvelinus fontinalis, Salmo trutta, Salmo salar and Thymallus thymallus. Appl Ichthyol 26:665–667

    Article  Google Scholar 

  • Cosson J, Linhart O (1996) Paddlefish (Polyodon spathula) spermatozoa: effects of potassium and pH on motility. Folia Zool 45(4):361–370

    Google Scholar 

  • Dettlaff TA, Ginsburg AS, Schmalhausen OI (1993) Sturgeon fishes, developmental biology and aquaculture. Verlag Berlin Heidelberg printed in. Springer, Germany

    Google Scholar 

  • Gallis JL, Fedrigo E, Jatteau P, Bonpunt E, Billard R (1991) Effects of dilution, pH, osmotic pressure, sodium and potassium ions on motility. In: Acipenser. In: Williot P (ed) Siberian sturgeon, Acipenser baeri, spermatozoa. Cemagref, Bordeaux, pp 143–151

    Google Scholar 

  • Ginsburg AS (1968) Fertilization in fishes and the problem of polyspermy. Izdatelnaya Nauka, Mosco

    Google Scholar 

  • Glogowski J, Kolmn R, Szczepkowski M, Horvath A, Urbanyi B, Scieczynski P, Rzemieniecki A, Domagala J, Demianowicz W, Kowalski R, Ciereszko A (2002) Fertilising rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture 211:367–373

    Article  CAS  Google Scholar 

  • Hanjavanit C, Kitancharoen N, Rakmanee C (2008) Experimental infection of aquatic fungi on eggs of African catfish (Clarias gariepinus Burch). J KKU Sci 36:36–43

    Google Scholar 

  • Ingermann RL, Holcomb M, Robinson ML, Cloud JG (2002) Carbon dioxide and pH affect sperm motility of white sturgeon (Acipenser transmontanus). Exp Biol 205:2885–2890

    CAS  Google Scholar 

  • Kjørsvik E, Mangor-Jensen A, Holmefjord I (1990) Egg quality in fishes. Adv Mar Biol 26:71–113

    Article  Google Scholar 

  • Linhart O, Mims AD, Shelton WL (1995) Motility of spermatozoa from shovelnose sturgeon (Scaphirhynchus platorynchus Rafinesque, 1820) and paddlefish (Polyodon spathula Walbaum, 1972). Fish Biol 47:902–909

    Article  Google Scholar 

  • Morisawa S, Morisawa M (1986) Acquisition of potential for sperm motility in rainbow trout and chum salmon. Exp Biol 126:89–96

    CAS  Google Scholar 

  • Morisawa S, Morisawa M (1988) Induction of potential for sperm motility by bicarbonate and pH in rainbow trout and chum salmon. Exp Biol 136:13–22

    CAS  Google Scholar 

  • Nazari RM, Sohrabnejad M, Ghomi MR (2009) The effect of maternal size on larval characteristics of Persiansturgeon Acipenser persicus. Aquac Res 40:1083–1088

    Article  Google Scholar 

  • Ottesen OH, Babiak L (2007) Parental effects on fertilization and hatching success and development of Atlantic halibut (Hippoglossus hippoglossus L.) embryos and larvae. Theriogenology 68:1219–1227

    Article  CAS  PubMed  Google Scholar 

  • Pool WR, Dillane MG (1998) Estimation of sperm concentration of wild and reconditioned brown trout, Salmon trutta L. Aquac Res 29:439–445

    Article  Google Scholar 

  • Stoss J (1983) Fish gamete preservation and spermatozoan physiology. In: Hoar WS, Randall DJ, Donaldson EM (eds) Fish physiology. Academic, NewYork, pp 305–350

    Google Scholar 

  • Tsvetkova LI, Cosson J, Linhart O, Billard R (1996) Motility and fertilizing capacity of fresh and frozen-thawed spermatozoa in sturgeon (Acipenser baeri and A. ruthenus). Journal of Applied Ichthyology 12:107–112

    Article  Google Scholar 

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Acknowledgments

The authors would like to thank Mr. Hossein Mohammadi for technical assistance during sperm sampling. We also gratefully acknowledge the head and staff of Dr. Dadman International Sturgeon Research Institute (Rasht) for their valuable contributions.

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Authors and Affiliations

  1. Department of Fishery Science, Faculty of Natural Resources, Islamic Azad University of Lahijan, P.O. Box 1616, Lahijan, Iran

    Hadiseh Dadras & Hosein Khara

  2. International Sturgeon Research Institute, P.O. Box: 41635-3464, Rasht, Iran

    Shahrouz Baradaran Noveiri

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  1. Hadiseh Dadras
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  2. Hosein Khara
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  3. Shahrouz Baradaran Noveiri
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Correspondence to Hadiseh Dadras.

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Dadras, H., Khara, H. & Noveiri, S.B. Effect of sperm pH and density on fertilization success in Persian sturgeon Acipenser persicus (Borodin, 1897). Comp Clin Pathol 23, 975–978 (2014). https://doi.org/10.1007/s00580-013-1727-3

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  • DOI: https://doi.org/10.1007/s00580-013-1727-3

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