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In vitro comparison of soybean lecithin-based extenders for cryopreservation of buffalo (Bubalus bubalis) semen

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Abstract

The objective of the present study was to compare the quality of frozen buffalo semen processed in tris-citric egg yolk extender with two different commercially available soybean lecithin-based extenders. Split pooled ejaculates, possessing more than 70 % visual sperm motility were divided in three aliquots and diluted in AndroMed®, Bioxcell®, or tris-citric egg yolk (TCE) extenders. Post-thaw motion characteristics, viability, plasma membrane integrity, acrosome morphology, and DNA integrity of buffalo sperm were studied after thawing and incubation for 4 h. Results demonstrate that total motility in frozen–thawed semen processed in TCE extender were similar when compared with AndroMed® or Bioxcell® extenders (P > 0.05). Kinematic parameters, such as average path velocity and linearity index in the soybean lecithin-based extenders were comparatively superior to TCE extender. After 4 h of incubation, proportions of overall and progressive motility decreased in all extenders but were comparatively superior in soybean lecithin-based extenders than TCE extender (P < 0.05). The proportion of post-thaw sperm viability, plasma membrane integrity and normal apical ridge remained similar in all extenders (P > 0.05). The post-thaw sperm viability was comparatively superior in soybean lecithin-based extenders than TCE after incubation at 37 °C for 4 h (P < 0.05). The type of extender had not been the significant effect on the percentage of spermatozoa with DNA damage after thawing and incubation for 4 h. In addition, our results suggest that soybean lecithin base extenders can be used as a substitute for tris-citric egg yolk extender in cryopreservation of buffalo semen.

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Acknowledgments

The authors would like to thank the director and station staff of buffalo breeding and extension training center for providing facilities and kind assistance throughout the experiment and the Royan Institute for the financial support and kind provision of materials to perform this experiment.

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran

    A. Rastegarnia & T. Rezaei topraggaleah

  2. Department of Embryology, Royan Institute for Reproductive Biomedicine Research Center, ACECR, Tehran, Iran

    A. Shahverdi & T. Rezaei topraggaleah

  3. Buffalo Breeding and Extension Training Center, Jabal, Urmia, Iran

    V. Shafiepour

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  1. A. Rastegarnia
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  2. A. Shahverdi
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  3. T. Rezaei topraggaleah
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Correspondence to A. Rastegarnia.

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Rastegarnia, A., Shahverdi, A., topraggaleah, T.R. et al. In vitro comparison of soybean lecithin-based extenders for cryopreservation of buffalo (Bubalus bubalis) semen. Comp Clin Pathol 23, 893–900 (2014). https://doi.org/10.1007/s00580-013-1708-6

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  • DOI: https://doi.org/10.1007/s00580-013-1708-6

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