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Storage-associated changes in the bovine hemogram with the ADVIA 120 hematology analyzer

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Delayed hematological analysis occurs frequently in bovine practice. Interpretation of complete blood count (CBC) results may be affected by artifactual storage-associated changes. The objective of this study was to characterize changes in bovine CBC results that occur over 72 h of storage at room temperature (RT) and 4 °C. Blood samples from 10 clinically healthy Holstein cows were analyzed with the ADVIA 120 hematology analyzer at 0, 24, 48, and 72 h of storage at RT or 4 °C. A two-way repeated-measure ANOVA was used to analyze time and temperature main effects. Time-associated changes in red blood cell (RBC) results were a significant increase in mean corpuscular volume (MCV) (both temperatures) and hematocrit (RT) and a decrease in mean corpuscular hemoglobin concentration (RT). White blood cell counts were relatively stable; however, automated absolute concentrations of monocytes and large unstained cells decreased, whereas concentrations of lymphocytes and eosinophils (RT) increased, over time. There was a statistically significant increase in mean platelet volume (MPV) by 48 h of storage at both temperatures. Changes with storage were more pronounced at RT. Mean CBC results, with the exception of MPV, did not exceed reference intervals. With storage, bovine RBCs become macrocytic and hypochromic, variable changes occur in the automated differential cell count, and the MPV increases. However, most changes, with the exception of MCV, are of little diagnostic relevance. For the most accurate CBC results, bovine blood should be stored at 4 °C and analyzed within 24 h of collection.

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The authors wish to thank the clinical pathology staff at Cornell University for analyzing the blood with the ADVIA 120 and the clinical pathology laboratory manager, Richard DeFrancisco, for data input.

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Correspondence to Amy L. Warren.

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This study was performed at the College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.

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Warren, A.L., Stokol, T., Hecker, K.G. et al. Storage-associated changes in the bovine hemogram with the ADVIA 120 hematology analyzer. Comp Clin Pathol 22, 1235–1240 (2013).

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