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Antimicrobial effects of the extracts of Zataria multiflora on the growth and multiplication of Listeria monocytogenes in milk using PCR


In a series of experiments, the possible antimicrobial role of Zataria multiflora was assessed against Listeria monocytogenes. Results presented here show the effects of the herbal extract on excluding the microorganism in milk tank bulks at a concentration of 0.8 mg/ml, which was demonstrated in both minimum inhibitory concentration and PCR assays. Considering the importance of the transmissible antibacterial resistance among different bacterial strains, it can be suggested that the crude extract of Z. multiflora could possibly act as an anti-Listeria agent.

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  • Bagamboula CF, Uyttendaele M, Debevere J (2003) Antimicrobial effect of spices and herbs on Shigella sonnei and Shigella flexneri. J Food Prot 66(4):668–673

    PubMed  CAS  Google Scholar 

  • Burt S (2004) Essential oils: their antibacterial properties and potential application in foods—a review. Int Food Microbiol 94:223–253

    Article  CAS  Google Scholar 

  • Choobkar N, Soltani M, Ebrahimzadeh Mousavi HA, Akhondzadeh Basti A, Matinfar A (2010) Effect of Zataria multiflora Boiss essential oil on the growth of Staphylococcus aureus in the light salted fillets of silver carp (Hypophthalmichthys molitrix). Iranian J Fish Sci 9(3):352–359

    Google Scholar 

  • European Food Safety Authority (2010) The community summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008. EFSA J 8:1496

    Google Scholar 

  • Filiousis G, Johansson A, Frey J, Perreten V (2009) Prevalence, genetic diversity and antimicrobial susceptibility of Listeria monocytogenes isolated from open-air food markets in Greece. Food Control 20:314–317

    Article  CAS  Google Scholar 

  • Goulas AE, Kontominas MG (2007) Combined effect of light salting, modified atmosphere packaging and oregano essential oil on the shelf-life of sea bream (Sparus aurata): biochemical and sensory attributes. Food Chem 100:287–296

    Article  CAS  Google Scholar 

  • Holley RA, Patel D (2005) Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiol 22(4):273–292

    Article  CAS  Google Scholar 

  • Lamont RF, Sobel J, Mazaki-Tovi S, Kusanovic JP, Vaisbuch S, Kim SK, Uldbjerg N, Romero R (2011) Listeriosis in human pregnancy: a systematic review. J Perinat Med 39:227–236

    PubMed  Article  Google Scholar 

  • Moradi M, Tajik H, Razavi Rohani SM, Oromiehie AR (2011) Effectiveness of Zataria multiflora Boiss essential oil and grape seed extract impregnated chitosan film on ready-to-eat mortadella-type sausage during refrigerated storage. J Sci Food Agric 91(15):2850–2857

    PubMed  Article  CAS  Google Scholar 

  • Moreira MR, Ponce AG, del Valle CE, Roura SI (2005) Inhibitory parameters of essential oils to reduce a foodborne pathogen. Lebensm Wiss Technol 38(5):565–570

    Article  CAS  Google Scholar 

  • Morvan A, Moubareck C, Leclercq A, Herve-Bazin M, Bremont S, Lecuit M, Courvalin P, Le Monnier A (2010) Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France. Appl Environ Microbiol 77(8):2788–2790

    Google Scholar 

  • Motalebi AA, Hasanzati Rostami A, Khanipour AA, Soltani M (2010) Impacts of whey protein edible coating on chemical and microbial factors of gutted kilka during frozen storage. Iranian J Fish Sci 9(2):255–264

    Google Scholar 

  • Niederhauser C, Candrian U, Höfelein C, Jermini M, Bühler HP, Lüthy J (1992) Use of polymerase chain reaction for detection of Listeria monocytogenes in food. Appl Environ Microbiol 58:1564–1568

    PubMed  CAS  Google Scholar 

  • Roberts MC, Facinelli B, Giovanetti E, Varaldo PE (1996) Transferable erythromycin resistance in Listeria spp. isolated from food. Appl Environ Microbiol 62:269–270

    PubMed  CAS  Google Scholar 

  • Rostamzad H, Shabanpour B, Kashaninejad M, Shabani A (2010) Inhibitory impacts of natural antioxidants (ascorbic and citric acid) and vacuum packaging on lipid oxidation in frozen. Iranian J Fish Sci 9(2):279–292

    Google Scholar 

  • Sharififar F, Moshafi MH, Mansouri SH, Khodashenas M, Khoshnoodi M (2007) In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control 18:800–805

    Article  CAS  Google Scholar 

  • Singh A, Singh RK, Bhunia AK, Singh N (2003) Efficacy of plant essential oils as antimicrobial agents against Listeria monocytogenes in hotdogs. Lebensm Wiss Technol 36(8):787–794

    Article  CAS  Google Scholar 

  • Singh N, Singh RK, Bhunia A, Stroshine RL (2002) Efficacy of chlorine dioxide, ozone, and thyme essential oil or a sequential washing in killing Escherichia coli O157: H7 on lettuce and baby carrots. Lebensm Wiss Technol 35(8):720–729

    Article  CAS  Google Scholar 

  • Vela AI, Fernandez-Garayzaball JF, Vazquez JA, Latre MV, Blanco MM, Moreno MA, de la Fuente L, Marco J, Franco C, Cepeda A, Rodriguez Moure AA, Suarez G, Dominguez L (2001) Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates. Appl Environ Microbiol 67(12):5840–5843

    PubMed  Article  CAS  Google Scholar 

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The work was financially supported by School of Veterinary Medicine, Shiraz University.

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Correspondence to H. Jesmani.

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Jesmani, H., Hosseinzadeh, S., Panahi, N. et al. Antimicrobial effects of the extracts of Zataria multiflora on the growth and multiplication of Listeria monocytogenes in milk using PCR. Comp Clin Pathol 22, 1025–1028 (2013).

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  • Zataria multiflora
  • Listeria monocytogenes
  • Minimum inhibitory concentration (MIC)
  • Milk