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Comparison of the ProCyte Dx analyzer with the ADVIA 2120 and the manual differential for validation of equine and bovine hemograms

Abstract

The aim of this study was to evaluate the laser-based in-house hematology analyzer ProCyte Dx performing a complete hemogram for bovine and equine blood samples. This is a prospective study of clinically healthy or ill horses (n = 175) and cattle (n = 115). EDTA-anticoagulated blood samples were analyzed within 3–6 h after sampling. Routine hemogram variables including differential counts were compared to reference methods (ADVIA 2120, 200-cell manual differential leukocyte count). Statistical analysis was performed twice, i.e., prior to and after exclusion of specimens with graphical reports (dot plots) indicative of invalid separation of cellular populations. Coefficient of variation was <3 % (CBC) and <7 % (differential count) except for eosinophils and monocytes (cattle) and platelets (PLTs) (horse). Linearity was excellent except for equine platelets. Spearman's rank correlation coefficients (r s) revealed a good to excellent (r s = 0.99–0.80) correlation between both analyzers for the majority of variables except for mean corpuscular hemoglobin concentration (MCHC) (r s = 0.33–0.65), equine platelets assessed with impedance count (r s = 0.79), and monocytes (both species). Biases were close to 0; however, for MCHC, hemoglobin (HGB), and PLTs large biases were seen for both species. There was a favorable correlation with the reference methods for the majority of variables. The large biases of HGB and HGB-derived variables were caused by the methodology of the ADVIA. As for other analyzers, correct PLT and monocyte count are difficult. Dot plot analysis is a useful tool to detect invalid separation of cellular populations and thus the necessity to perform a manual differential count.

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Notes

  1. Idexx, Westbrook, Maine

  2. Sysmex Corporation, Kobe, Japan

  3. Abbot Laboratories, Abbot Park, IL, USA

  4. Siemens, Medical solutions diagnostics, Eschborn, Germany

  5. Hematokrit 24, Hettich Zentrifugen, Tuttlingen, Germany

  6. RET search II, Sysmex, Norderstedt, Germany

  7. Stomatolyser 4DS, Sysmex, Norderstedt, Germany

  8. E-Check (XE), Sysmex, Norderstedt, Germany

  9. Analyze-it Software Ltd, Leeds, UK

  10. GraphPad Software Inc, La Jolla, CA, USA

Abbreviations

CBC:

Complete blood cell count

FSC:

Forward scatter

EOS:

Eosinophils

HGB:

Hemoglobin

HTC:

Hematocrit value

MCH:

Mean corpuscular hemoglobin

MCV:

Mean corpuscular volume

LYMPH:

Lymphocytes

MCHC:

Mean corpuscular hemoglobin concentration

MONO:

Monocytes

NEUT:

Neutrophils

PCV:

Packed cellular volume

PLT-I:

Platelets measured with impedance method

PLT-O:

Platelets measured with optical method

RBC:

Red blood cells

r s :

Spearmann's rho

SSC:

Side scatter

SFL:

Side fluorescence

WBC:

White blood cells

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Correspondence to Natali Bauer.

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Goldmann, F., Bauer, N. & Moritz, A. Comparison of the ProCyte Dx analyzer with the ADVIA 2120 and the manual differential for validation of equine and bovine hemograms. Comp Clin Pathol 22, 855–868 (2013). https://doi.org/10.1007/s00580-012-1489-3

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  • DOI: https://doi.org/10.1007/s00580-012-1489-3

Keywords

  • Hematology
  • Laser-based
  • In-house hematology analyzer
  • Large animals
  • Method validation