Comparative Clinical Pathology

, Volume 22, Issue 5, pp 823–827 | Cite as

Levels of malondialdehyde and 4-hydroxyalkenals in bovine liver biopsy

  • Mahmoud R. Abd EllahEmail author
  • Keiji Okada
  • Masanobu Goryo
  • Jun Yasuda
Original Article


The present study was designed to elucidate if ethionine administration could be used to induce hepatic lipoperoxidation in cattle, as indicated by the increase concentrations of the lipid peroxidation products, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HAE) in the hepatic tissues. Five cows were injected intraperitoneally with dl-ethionine (12.5 mg/kg body weight). Liver biopsy specimens were collected at the 0, 4th, 7th and 10th day after ethionine administration, and were used for measuring hepatic lipid peroxidation products (MDA and 4-HAE) levels and for histopathological examination. Statistical analysis was carried out by comparing data from days 4, 7 and 10 with those from day 0. Different degrees of cell swelling and glycogen degeneration were developed in the liver tissues on days 4, 7 and 10 after ethionine administration. The results revealed significant increases in hepatic lipid peroxidation products (p < 0.05) on day 7. In the experimental model system described in this study, dl-ethionine administration was effective in inducing hepatic lipoperoxidation in cattle as indicated by altered concentrations of hepatic lipid peroxidation products and by histopathological examination of liver biopsies.


Bovine MDA 4-HAE Liver Biopsy 



This work was supported by Grants-in-Aid for Basic Scientific Research (No. 17–05483) from the Ministry of Education, Culture, Sports, Science and Technology, Government of Japan and by The Japanese Society for the Promotion of Science.


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Copyright information

© Springer-Verlag London Limited 2012

Authors and Affiliations

  • Mahmoud R. Abd Ellah
    • 1
    Email author
  • Keiji Okada
    • 2
  • Masanobu Goryo
    • 2
  • Jun Yasuda
    • 2
  1. 1.Department of Animal Medicine, Faculty of Veterinary MedicineAssiut UniversityAssiutEgypt
  2. 2.Veterinary Teaching Hospital, Faculty of AgricultureIwate UniversityMoriokaJapan

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