Antidiabetic effect of Orchis anatolica root extracts on alloxan-induced diabetic rats

Abstract

The aim of our study was to evaluate the effect of Orchis anatolica roots ethanol extraction on alloxan-induced diabetic rats. Thirty-six albino rats (200 g) were used in this experiment and divided into six groups. Diabetes was induced in five rat groups by a single intraperitoneal injection of alloxan (150 mg/kg body weight). After hyperglycemia was conformed, one rat group was considered as diabetic control and one group was treated with glibenclamide (10 mg/kg body weight/daily) where the remaining three groups received daily treatments with three different doses of O. anatolica extract namely 200, 400, and 800 mg/kg body weight for 10 days. Body weight and fasting blood sugar levels were recorded throughout and by the end of the treatment. Blood serum biochemical markers such as urea, creatinine, cholesterol, and total serum protein levels were recorded after the treatment ended. Findings indicate that treatment with medium and high doses of O. anatolica extract (400 and 800 mg/kg/body weight) reduces blood sugar values to significant levels (P < 0.01 and P < 0.001) in rats after 7 and 10 days treatment when compared with diabetic control alloxan-induced rats in a similar fusion as in glibenclamide treatment (P < 0.001). However, both treatments failed to bring the blood sugar values to normal value levels. All elevated blood serum markers induced by the alloxan treatment were reduced to significant levels in rats treated with O. anatolica at both medium and high doses (P < 0.01 and P < 0.001) and also after glibenclamide treatment (P < 0.001). Glibenclamide and the two high doses of O. anatolica extract did not alter the rat’s body weight when compared with nondiabetic control rats, whereas significant reduction (P < 0.05) was observed when compared with the alloxan-induced rats’ body weight. We can conclude that O. anatolica treatment exhibits a significant antihyperglycemic effect without altering the body weight and can correct some biochemical uttered markers induced by diabetes in a similar manner to glibenclamide treatment.

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Acknowledgment

This research was funded by the Dean’s Office of Research at JUST through the grant number 26/2010.

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Correspondence to Nabil A. Khouri.

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Khouri, N.A., Daradka, H. Antidiabetic effect of Orchis anatolica root extracts on alloxan-induced diabetic rats. Comp Clin Pathol 22, 347–354 (2013). https://doi.org/10.1007/s00580-012-1415-8

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Keywords

  • Alloxan
  • Diabetes
  • Orchis anatolica
  • Blood glucose
  • Antihyperglycemic effect
  • Biochemical markers