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Comparative Clinical Pathology

, Volume 21, Issue 6, pp 1689–1696 | Cite as

Allelic variants of insulin receptor substrate-1 gene in Egyptian women with polycystic ovary syndrome

  • Fatma A. Elmougy
  • Marianne F. Morgan
  • Dina F. Elgayar
  • Abeer M. Mohey
  • Heba N. Baz
  • Ahmed M. Ali
Original Article
  • 66 Downloads

Abstract

The insulin receptor substrate gene-1 (IRS-1) polymorphisms have been incriminated in insulin resistance; a frequent finding in polycystic ovary syndrome (PCOS). The commonest polymorphism associated with insulin resistance is gly972arg. Another polymorphism is the ala512 pro. This study aimed at elucidating the association of the gly972arg and the ala512pro polymorphisms of the IRS-1 gene with PCOS in Egyptian females. We employed a case–control study. The two polymorphisms were analyzed in 62 PCOS Egyptian female patients and 43 age-matched controls using a PCR-RFLP strategy. Fasting insulin, plasma glucose, and testosterone were analyzed by routine analytical methods and calculation of the homeostasis model assessment (HOMA) and the quantitative sensitivity check index (QUICK I) were done based on the results of the fasting insulin and glucose. It was found that 6.5% of the PCOS cases showed the Gly972Arg genotype. The Gly972Arg in IRS-1 polymorphism was not associated with PCOS (p = 0.556). A positive association was found between the gly972arg and the HOMA quartiles (p = 0.01). PCOS patients with the 972gly/arg genotype had higher glucose (p = 0.040), insulin (0.000), HOMA (0.000), and lower QUICK I (0.023) than PCOS with wild 972arg/arg genotype. Patients of PCOS with the 972gly/arg genotype were more insulin resistant than other patients with the wild genotype. Neither the patients nor the controls showed the mutant ala512pro genotype. Screening for gly972arg genotype can be based on the HOMA quartile ranking. The ala512pro genotype was not found in studied Egyptian women.

Keywords

PCOS Insulin resistance gly972arg Polymorphism ala512pro Polymorphism 

Notes

Conflict of interest

None

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Copyright information

© Springer-Verlag London Limited 2011

Authors and Affiliations

  • Fatma A. Elmougy
    • 1
  • Marianne F. Morgan
    • 1
  • Dina F. Elgayar
    • 1
    • 3
  • Abeer M. Mohey
    • 1
  • Heba N. Baz
    • 1
  • Ahmed M. Ali
    • 2
  1. 1.Department of Clinical and Chemical Pathology, Faculty of MedicineCairo UniversityGizaEgypt
  2. 2.Department of Obstetric and Gynecology, Faculty of MedicineCairo UniversityGizaEgypt
  3. 3.GizaEgypt

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