Effect of exposure to perfluorooctanoic acid on hepatic antioxidants in mice

Abstract

The present study was undertaken to evaluate hepatic antioxidants status in female mice after exposure to perfluorooctanoic acid (PFOA). A total number of 20 female mice were subjected to the study; out of them, 15 mice were treated with PFOA. PFOA solutions were prepared at 0.1, 0.5, and 1 mg/ml of deionized water and administered to the mice by gavage once daily for 3 weeks at a volume of 10 ml/kg. Controls (five mice) received an equivalent volume of deionized water. The results revealed significant increase in liver weight at dose of 5 (p < 0.01) and 10 mg/kg body weight (b.w., p < 0.01). Both hepatic total glutathione level and hepatic catalase activity were significantly increased (p < 0.01) at dose of 5 mg/kg b.w. On the other hand, hepatic glutathione reductase activity was significantly increased at dose of 10 mg/kg b.w. In this model system, PFOA administration was effective in inducing oxidative stress particularly reflected in the liver.

This is a preview of subscription content, access via your institution.

References

  1. Abd Ellah MR (2010) Involvement of free radicals in animal diseases. Comp Clin Pathol 19:615–619

    Article  Google Scholar 

  2. Abd Ellah MR (2011) The role of liver biopsy in detection of hepatic oxidative stress. Review article. Vet Med Int. doi:10.4061/2011/613602, 2011: Article ID 613602

  3. Anna-Karin S, Karin A, Joseph WD (1992) The effects of perfluoro-octanoic acid on hepatic peroxisome proliferation and related differences in mice parameters show no sex-related. Biochem J 285:779–783

    Google Scholar 

  4. Bossi R, Riget FF, Dietz R, Sonne C, Fauser P, Dam M, Vorkamp K (2005) Preliminary screening of perfluorooctane sulfonate (PFOS) and other fluorochemicals in fish, birds and marine mammals from Greenland and the Faroe Islands. Environ Pollut 36:323–329

    Article  Google Scholar 

  5. DeLeve LD, Kaplowitz N (1991) Glutathione metabolism and its role in hepatotoxicity. Pharmacol Ther 52:287–305

    PubMed  Article  CAS  Google Scholar 

  6. Ericson I, Gomez M, Nadal M, Van Bavel B, Lindstrom G, Domingo JL (2007) Perfluorinated chemicals in blood of residents in Catalonia (Spain) in relation to age and gender: a pilot study. Environ Int 33:616–623

    PubMed  Article  Google Scholar 

  7. Eriksson AM, Zetterqvist MA, Lundgren B, Andersson K, Beije B, DePierre JW (1991) Studies on the intracellular distributions of soluble epoxide hydrolase and of catalase by digitonin-permeabilization of hepatocytes isolated from control and clofibrate-treated mice. Eur J Biochem 198:471–476

    PubMed  Article  CAS  Google Scholar 

  8. Giesy JP, Kannan K (2001) Global distribution of perfluorooctane sulfonate in wildlife. Environ Sci Technol 35:1339–1342

    PubMed  Article  CAS  Google Scholar 

  9. Higgins CP, Field JA, Criddle CS, Luthy RG (2005) Quantitative determination of perfluorochemicals in sediments and sludge. Environ Sci Technol 39:3946–3956

    PubMed  Article  CAS  Google Scholar 

  10. Kannan K, Choi J, Iseki N, Senthilkumar K, Kim DH, Masunaga S, Giesy JP (2002) Concentrations of perfluorinated acids in livers of birds from Japan and Korea. Chemosphere 49:225–231

    PubMed  Article  CAS  Google Scholar 

  11. Key BD, Howell RD, Criddle CS (1997) Fluorinated organics in the biosphere. Environ Sci Technol 31:2445–2454

    Article  CAS  Google Scholar 

  12. Klucis E, Crane D, Masters C (1984) Sequential alterations in the micro-localization of catalase in mouse liver after treatment with hypolipidemic drugs. Mol Cell Biochem 65:73–82

    PubMed  Article  CAS  Google Scholar 

  13. Livingstone DR (2001) Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar Pollut Bull 42:656–666

    PubMed  Article  CAS  Google Scholar 

  14. Loveless SE, Finlay C, Everds NE, Frame SR, Gillies PJ, O'Connor JC, Powley CR, Kennedy GL (2006) Comparative responses of rats and mice exposed to linear/branched, linear, or branched ammonium perfluorooctanoate (APFO). Toxicology 220:203–217

    PubMed  Article  CAS  Google Scholar 

  15. Lunec J (1990) Free radicals: their involvement in disease process. Ann Clin Biochem 27:17–182

    Google Scholar 

  16. Martin JW, Smithwick MM, Braune BM, Hoekstra PF, Muir DCG, Mabury SA (2004) Identification of long-chain perfluorinated acids in biota from the Canadian arctic. Environ Sci Technol 38:373–380

    PubMed  Article  CAS  Google Scholar 

  17. Mitchell AM, Bridges JW, Elcombe CR (1984) Factors influencing peroxisome proliferation in cultured rat hepatocytes. Arch Toxicol 55:239–246

    PubMed  Article  CAS  Google Scholar 

  18. Samuni A, Chevion M, Czapski G (1981) Unusual copper induced sensitization of the biological damage to superoxide radicals. J Biol Chem 256:12632–12635

    PubMed  CAS  Google Scholar 

  19. Shabalina IG, Panaretakis T (1999) Effects of the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid, on apoptosis in human hepatoma HepG2 cells. Carcinogenesis 20:2237–2246

    PubMed  Article  CAS  Google Scholar 

  20. So MK, Miyake Y, Yeung WY, Ho YM, Taniyasu S, Rostkowski P, Yamashita N, Zhou BS, Shi XJ, Wang JX, Giesy JP, Yu H, Lam PKS (2007) Perfluorinated compounds in the Pearl River and Yangtze River of China. Chemosphere 68:2085–2095

    PubMed  Article  CAS  Google Scholar 

  21. So MK, Taniyasu S, Yamashita N, Giesy JP, Zheng J, Fang Z, Im SH, Lam PKS (2004) Perfluorinated compounds in coastal waters of Hong Kong, South China, and Korea. Environ Sci Technol 38:4056–4063

    PubMed  Article  CAS  Google Scholar 

  22. Sohlenius AK, Andersson K, Bergstrand A, Spydevold O, DePierre JW (1994) Effects of perfluorooctanoic acid—a potent peroxisome proliferator in rat—on Morris hepatoma 7800C1 cells, a rat cell line. Biochim Biophys Acta 1213:63–74

    PubMed  Article  CAS  Google Scholar 

  23. Sohlenius AK, Eriksson AM, Högström C, Kimland M, DePierre JW (1993) Perfluorooctane sulfonic acid is a potent inducer of peroxisomal fatty acid beta-oxidation and other activities known to be affected by peroxisome proliferators in mouse liver. Pharmacol Toxicol 72:90–93

    PubMed  Article  CAS  Google Scholar 

  24. Son HY, Kim SH, Shin HI, Bae HI, Yang JH (2008) Perfluorooctanoic acid-induced hepatic toxicity following 21-day oral exposure in mice. Arch Toxicol 82:239–246

    PubMed  Article  CAS  Google Scholar 

  25. Volkel W, Genzel-Boroviczeny O, Demmelmair H, Gebauer C, Koletzko B, Twardella D, Raab U, Fromme H (2008) Perfluorooctane sulphonate (PFOS) and perfluorooctanoic acid (PFOA) in human breast milk: results of a pilot. Int J Hyg Environ Heal 211:440–446

    Article  CAS  Google Scholar 

  26. Wang W, Ballatori N (1998) Endogenous glutathione conjugates: occurrence and biological functions. Pharmacol Rev 50:335–355

    PubMed  CAS  Google Scholar 

  27. Xiao-Zhong Hu, De-Cong Hu (2009) Effects of perfluorooctanoate and perfluorooctane sulfonate exposure on hepatoma Hep G2 cells. Arch Toxicol 83:851–861

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Mahmoud R. Abd Ellah.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ahmed, D.Y., Abd Ellah, M.R. Effect of exposure to perfluorooctanoic acid on hepatic antioxidants in mice. Comp Clin Pathol 21, 1643–1645 (2012). https://doi.org/10.1007/s00580-011-1341-1

Download citation

Keywords

  • PFOA
  • Mice
  • Antioxidants
  • Liver