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Characterization of the siderophore transport genes in Aeromonas salmonicida subsp. salmonicida

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Abstract

Bacteria have developed multiple mechanisms to obtain iron from the host. Aeromonas salmonicida subsp. salmonicida produces a catechol-type siderophore under iron starvation conditions. In this study, we characterized six ABC transporter proteins immediately downstream of a sigma factor as AsbI, which were denominated AsbH, AsbJ, AsbK, AsbL, AsbQ, and AsbM, and a siderophore receptor as AsbE. All studied genes showed a high similarity with other siderophore transporter genes in other gram-negative bacteria. Interestingly, asbH and asbJ genes were shown to encode a mixed-permease, ATP-binding protein. Reverse transcriptase PCR analysis demonstrated that the gene cluster is arranged into two transcriptional units: (1) asbIHJKLQM and (2) asbE. It also showed that the expression of these genes is regulated by iron concentration.

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Authors and Affiliations

  1. Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

    Mohsen Najimi

  2. Department of Microbiology, University of Zabol, Zabol, Iran

    Mohsen Najimi

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  1. Mohsen Najimi
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Correspondence to Mohsen Najimi.

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Najimi, M. Characterization of the siderophore transport genes in Aeromonas salmonicida subsp. salmonicida . Comp Clin Pathol 21, 1427–1431 (2012). https://doi.org/10.1007/s00580-011-1310-8

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  • DOI: https://doi.org/10.1007/s00580-011-1310-8

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