Abstract
Avocado/soybean mixture is shown to have a positive effect on cartilage preservation in arthritis. This study was undertaken to study the healing effect of soybean and avocado mixture on full-thickness knee cartilage defect, which were treated by microfracture in dog. Twenty-two outbred male dogs (44 knees) were divided into two equal groups. In both groups, a 1 × 1 cm full-thickness lesion was created in medial femoral condyle of each knee eliminating the calcified cartilage, which was curetted down to the subchondral bone. In the left knee, a microfracture was produced. The right knee was closed without performing any microfracture. Half of the dogs in each group received 300 mg of avocado/soybean mixture in one capsule, and the remaining animals in each group received a placebo as an empty capsule each day. There were four treatment groups, including a combined group undergoing microfracture and receiving avocado/soybean, microfracture group undergoing microfracture and receiving placebo, avocado/soybean group that did not undergo microfracture but received avocado/soybean, and a control group that did not undergo microfracture and received placebo. There was no postoperative limitation of motion. After 12 weeks, all dogs were euthanized, and the articular cartilage tissues were studied according to International Cartilage Repair Society (ICRS) visual histological assessment scale. There were no significant differences in cartilage surface histology, cell distribution, and cell viability among the four groups. Subchondral bone histology was normal in six specimens in the control and avocado/soybean groups. One in the microfracture group and two in the combined group achieved normal histology. Four samples in each of the control and avocado/soybean mixture and combined groups, and nine samples in the microfracture group depicted increased remodeling of subchondral bone. Calcified cartilage zones were normal in six and seven samples in the control and the avocado/soybean mixture groups, respectively. There were no samples with normal histological appearance in the microfracture group. We did not observe any additive effect of avocado/soybean mixture to microfracture induced repair tissue in this study as evaluated by ICRS visual histological assessment scale.
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References
Boumediene K, Felisaz N, Bogdanowicz P, Galera P, Guillou GB, Pujol JP (1999) Avocado/soya unsaponifiables enhance the expression of transforming growth factor _1 and _2 in cultured articular chondrocytes. Arthritis Rheum 42:148–156
Cake MA, Read RA, Guillout B, Ghosh P (2000) Modification of articular cartilage and subchondral bone pathology in an ovine meniscectomy model of osteoarthritis by avocado and soya unsaponifiables (ASU). Osteoarthritis Cartilage 8(6):404–411
Frisbie DD, Oxford JT, Southwood L, Trotter GW, Rodkey WG, Steadman JR, Goodnight JL, McIlwraith CW (2003) Early events in cartilage repair after subchondral bone microfracture. Clin Orthop 407:215–227
Gill TJ, McCulloch PC, Glasson SS, Blanchet T, Morris EA (2005) Chondral defect repair after microfracture procedure: a nonhuman primate model. Am J Sport Med 33(5):680–685
Gill TJ, Asnis PD, Berkson EM (2006) The treatment of articular cartilage defect using the microfracture technique. J Orthop Sport Phys Ther 36(10):728–738
Henrotin YE, Labasse AH, Jaspar JM, De Groote DD, Zheng SX, Guillou GB, Reginster JY (1998) Effects of three avocado/soybean unsaponifiable mixtures on metalloproteinases, cytokines and prostaglandine E2 production by human articular chodrocytes. Clin Rheumatol 17(1):31–39
Henrotin Y, Michelle AD, Crielaard JM, Piccardi N, Msika P, Sanchez C (2006) Avocado/Soybean unsaponifiables prevent the inhibitory effect of osteoarthritic subchondral osteoblasts on aggrecan and type II collagen synthesis by chondrocytes. J Rheumatol 33(8):1668–1678
Mainil-Varlet P, Aigner T, Brittberg M, Bullough P, Hollander A, Hunziker E, Kandel R, Nehrer S, Pritzker K, Roberts S, Stauffer E, International Cartilage Repair Society (2003) Histologic assessment of cartilage repair. A report by histology endpoint committee of the international cartilage repair society (ICRS). J Bone Joint Surg 85A:45–57
Mankin HJ, Dorfman H, Lippiellol L, Zarrins A (1971) Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg 53A:523–537
Marder RA, Hopkins G Jr, Timmerman LA (2005) Arthroscopic microfracture of chondral defects of the knee: a comparison of two postoperative treatments. Arthroscopy 21(2):152–158
Mithoefer K, Williams RJ 3rd, Warren RF, Potter HG, Spock CR, Jones EC, Wickiewicz TL, Marx RG (2005) The microfracture technique for the treatment of articular cartilage lesions in the knee. A prospective cohort study. J Bone Joint Surg Am 87(9):1911–1920
Newman AP (1998) Current concepts: articular cartilage repair. Am J Sport Medicine 26(2):309–324
O’Driscoll SW, Keely FW, Salter RB (1988) Durability of regenerated articular cartilage produced by free autogenous periosteal grafts in major full-thickness defects in joint surface under the influence of continuous passive motion. A follow-up report at one year. J Bone Joint Surg 70A:595–606
Radin EL, Martin RB, Burr DB, Caterson B, Boyd RD, Goodwin C (1984) Effects of mechanical loading on the tissues of the rabbit knee. J Orthop Res 2(3):221–234
Steadman JR, Rodkey WG, Briggs KK (2002) Microfracture to treat full-thickness chondral defects: surgical technique, rehabilitation, and outcomes. J Knee Surg 15:170–1706
Steadman JR, Miller BS, Karas SG, Schlegel TF, Briggs KK, Hawkins RJ (2003) The microfracture technique in the treatment of full-thickness chondral lesions of the knee in National Football League players. J Knee Surg 16:83–86
Vasara AI, Hyttinen MM, Lammi MJ, Lammi PE, Långsjö TK, Lindahl A, Peterson L, Kellomäki M, Konttinen YT, Helminen HJ, Kiviranta I (2004) Subchondral bone reaction associated with chondral defect and attempted cartilage repair in goats. Calcific Tissue Int 74:107–114
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The authors wish to thank the Office of Vice Chancellor for Research of Shiraz University of Medical Sciences for financial support.
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Jaberi, F.M., Tahami, M., Torabinezhad, S. et al. The healing effect of soybean and avocado mixture on knee cartilage defects in a dog animal model. Comp Clin Pathol 21, 661–666 (2012). https://doi.org/10.1007/s00580-010-1152-9
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DOI: https://doi.org/10.1007/s00580-010-1152-9