Abstract
The objective of this study was to determine the effect of storage time, temperature, and anticoagulant on hematologic parameters in equine blood samples. Blood samples were obtained from 50 clinically healthy warm-blooded horses at two major equestrian complexes in Tehran, Iran. The samples were collected in three different tubes containing EDTA, sodium citrate, or heparin and were analyzed within 4 h of collection. Blood samples collected into EDTA-containing tubes were stored at 4°C or 24°C. Each sample was analyzed again at 24, 48, and 72 h after collection. The statistically significant (P < 0.05) alterations included decreased RBC count and increased hemoglobin concentration [Hgb] in blood samples stored at 24°C after 48 and 72 h; increased hematocrit in blood samples stored at 24°C after 24 h; decreased hematocrit in blood samples stored at 4°C after 72 h; decreased MCHC in blood samples stored at 4°C after 72 h; and decreased total WBC count in blood samples stored at 24°C after 48 h. Although there was no significant difference in hematologic analytes between heparinized and EDTA-anticoagulated blood samples, most of the hematologic analytes were decreased significantly (P < 0.05) in sodium citrate-containing blood samples, compared with blood samples stored in EDTA. The results of this study suggest that within certain limitations for some hematologic analytes, equine blood samples stored in EDTA at 4°C for up to 72 h may be suitable for hematologic testing.
Similar content being viewed by others
References
Buttarello M (2004) Quality specification in haematology: the automated blood cell count. Clin Chim Acta 346(1):45–54
Clark P, Mogg TD, Tvedten HW, Korcal D (2002) Artifactual changes in equine blood following storage, detected using the Advia 120 hematology analyzer. Vet Clin Pathol 31:90–94
Cohle SD, Saleem A, Makkaoui DE (1981) Effects of storage of blood on stability of hematologic parameters. Am J Clin Pathol 76:67–69
Furlanello T, Tasca S, Caldin M, Carli E, Patron C, Tranquillo M, Lubas G, Solano-Gallego L (2006) Artifactual changes in canine blood following storage, detected using the ADVIA 120 hematology analyzer. Vet Clin Pathol 35:42–46
Guder WG (1999) Preanalytical factors and their influence on analytical quality specifications. Scand J Clin Lab Invest 59:545–549
Gulati GL, Hyland LJ, Kocher W, Schwarting R (2002) Changes in automated complete blood cell count and differential leukocyte count results induced by storage of blood at room temperature. Arch Pathol Lab Med 126:336–342
Hayashi Y, Matsuzawa T, Unno T, Morita N, Nomura M (1995) Effects on hematology parameters during cold storage and cold transport of rat and dog blood samples. Comp Haematol Int 5:251–255
Hedberg P, Lehto T (2009) Aging stability of complete blood count and white blood cell differential parameters analyzed by Abbott CELL-DYN Sapphire hematology analyzer. Int J Lab Hem 31:87–96
Ihedioha JI, Onwubuche RC (2007) Artifactual changes in PCV, hemoglobin concentration, and cell counts in bovine, caprine, and porcine blood stored at room and refrigerator temperatures. Vet Clin Pathol 36:60–63
Jain NC (1996) Schalm's veterinary hematology, 4th edn. Lea & Febiger, Philadelphia
Lippi G, Salvagno GL, Solero GP, Franchini M, Guidi GC (2005) Stability of blood cell counts, hematologic parameters and reticulocytes indexes on the Advia A120 hematologic analyzer. J Lab Clin Med 146:333–340
Médaille C, Briend-Marchal A, Braun JP (2006) Stability of selected hematology variables in canine blood kept at room temperature in EDTA for 24 and 48 hours. Vet Clin Pathol 35:18–23
National Committee for Clinical Laboratory Standards (1996) Evacuated tubes and additives for blood specimen collection, 4th edn. Approved standard. NCCLS Document H1-A4, Villanova, PA
Olsen AK, Bladbjerg EM, Jensen AL, Hansen AK (2001) Effect of pre-analytical handling on haematological variables in minipigs. Lab Anim 35:147–152
Prins M, van Leeuwen MW, Teske E (2009) Stability and reproducibility of ADVIA 120-measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin content (CH(R)) in the diagnosis of iron deficiency. Tijdschr Diergeneeskd 134:272–278
Wood BL, Andrews J, Miller S, Sabath DE (1999) Refrigerated storage improves the stability of the complete blood count and automated differential. Am J Clin Pathol 112:687–695
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sharif, M.T., Mahabadi, M.A., Moshfeghi, S. et al. Artifactual changes in hematological variables in equine blood samples stored at different temperatures and various anticoagulants. Comp Clin Pathol 21, 449–452 (2012). https://doi.org/10.1007/s00580-010-1116-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00580-010-1116-0