Comparative Clinical Pathology

, Volume 21, Issue 4, pp 389–394 | Cite as

In vitro antioxidant and in vivo antidiabetic potential of the methanol extract of Ficus glumosa Del (Moraceae) stem bark in alloxan-induced diabetic mice

  • Ifeanyi Innocent Madubunyi
  • Samuel Okwudili Onoja
  • Isaac Uzoma Asuzu
Original Article


Ficus glumosa Del (Moraceae) commonly called “African rock fig” is a large tree indigenous to southern part of Nigeria. Its stem bark has been of interest to researchers because of its use in the treatment of various disease conditions in Nigerian traditional medicine. The present study was aimed at evaluating the antidiabetic and antioxidant properties of the methanol extract of F. glumosa stem bark using alloxan-induced diabetic mice. The extract, at the dose of 62.5, 125, and 250 mg/kg showed a remarkable time-dependent decrease in blood glucose level in alloxan-induced diabetic mice. There was no significant difference between the extract-treated groups and the groups treated with 10 mg/kg distilled water and glibenclamide (2 mg/kg) respectively. At 6-h post-treatment, the blood glucose level for the groups treated with 62.5 mg/kg of F. glumosa and glibenclamide (2 mg/kg), respectively, were lower than the normal blood glucose level respectively for the groups before the induction of diabetes. F. glumosa showed its optimum antioxidant activity in DPPH spectrophotometric assay at the concentration of 100 μg/ml. The ferric reducing antioxidant power showed a significant concentration dependent increase in the total antioxidant power. These findings demonstrate that F. glumosa has both antidiabetic and antioxidant effects on experimental model of diabetes in mice and validate its use in Nigerian traditional medicine for the treatment of diabetes. Even in its crude form, the effects, especially at 62.5 mg/kg, were comparable to that of glibenclamide, an oral sulfonylurea with proven antidiabetic activity. This finding suggests that the extract could be a potential source of a novel antidiabetic and antioxidant agent for the treatment of diabetes mellitus.


Ficus glumosa stem bark Antidiabetic activity Blood glucose Antioxidant Alloxan 


  1. Anaga AO, Asuzu IU (2010) Antihyperglycaemic properties of the ethyl acetate extract of Dennettia tripetala in diabetic rats. J Complement Integr Med 7(1):1–11CrossRefGoogle Scholar
  2. Anaga AO, Njoku CJ, Ekejiuba ES, Esiaka MN, Asuzu IU (2004) Investigation of the methanolic leaf extract of Costus afer Ker for pharmacological activities in vitro and in vivo. Phytomedicine 11:242–248PubMedCrossRefGoogle Scholar
  3. Anaga AO, Shoyinka SVO, Asuzu IU (2006) Toxic effects of Dennettia tripetala root extract. Pharm Biol 44:451–461CrossRefGoogle Scholar
  4. Atkins SH, Dasmahapatra A, Jaker MA, Chorost MI, Reddy S (1991) Fingerstick glucose determination in shock. Ann Int Med 114:1020–1024Google Scholar
  5. Bansal R, Ahmad N, Kidwai JR (1980) Alloxan glucose interaction: effects of incorporation of 14C leucine into pancreatic islet of rats. Acta Diabetol Lat 17:135–143PubMedCrossRefGoogle Scholar
  6. Benzie FF, Strain JJ (1999) Ferric reducing/Antioxidant power Assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology. 299:15–23. In: Mayank, Reducing Antioxidant power (FRAP) Assay. Dairy cattle Nurtrition Division, NDRI, Karnal, India.Google Scholar
  7. DDHS (1985) Guide for the Care and Use of Laboratory Animals, Institute of Laboratory Animal Resources Commission on Life Sciences, National Research Council, National Academy Press, Washington, D.C.Google Scholar
  8. Burkill HM (1985) The useful plants of West Tropical Africa, vol. 1. Royal Botanic Gardens, Kew, pp 386–387Google Scholar
  9. Fabricant DS, Farnsworths NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect, Suppl 109(1):66–76CrossRefGoogle Scholar
  10. Fischer J (1985) Drugs and chemicals that produce diabetes. Trends Pharmacol Sci 6:72–75CrossRefGoogle Scholar
  11. Foster DW (1996) Diabetes mellitud. In: Isselbacher KJ, Brawnwald E, Wilson JD, Martin JB, Fauci AS, Kasper DL (eds) Harrison’s principle if internal medicine. McGraw Hill, New York, pp 1979–1981Google Scholar
  12. Ivorra MD, Paya M, Villar A (1989) A review of natural products and plants as potential antidiabetic drugs. J Ethnopharmacol 27:243–275PubMedCrossRefGoogle Scholar
  13. Iwalewa EO, Adewale IO, Taiwo BJ, Arogundabe T, Daniyan OA, OM AGE (2008) Effects of Harungana madagascariensis stem bark extract on the antioxidant markers in alloxan-induced diabetic and carrageenan-induced inflammatory disorders in rats. J Complement Integr Med 5(1):1–18Google Scholar
  14. King H, Aubert R, Herman WH (1998) Global budern of diabetes 1995–2025 prevalence, numerical estimates and projections. Diab Care 21:1414–1431CrossRefGoogle Scholar
  15. Marles RJ, Farnsworth NR (1994) Plant as sources of antidiabetic agents. In: Marles RJ, Farnsworth NR (eds) Economic and medicinal plant research. Academic Press Ltd, Illinois, pp 150–187Google Scholar
  16. Marxen K, Vanselow KH, Lippemeier S, Hintze R, Ruser A, Hansen U (2007) Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements. Sensors 7:2080–2095CrossRefGoogle Scholar
  17. Mensor LL, Fabio SM, Gilda GL, Alexandre SR, dos Santos TC, Cintia SC, Suzana GL (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapia Res 15:127–130CrossRefGoogle Scholar
  18. Nadkarni AN (1989) Indian Materia Medica, vol. 1. Popular Book Depot, Mumbai, p 544Google Scholar
  19. Rahman A, Zaman K (1989) Medicinal plants with hypoglycemic activity. J Ethnopharmacol 26:1–55CrossRefGoogle Scholar
  20. Sies H (1997) Oxidative stress: oxidants and antioxidants. Exp Physiol 82:291–295PubMedGoogle Scholar
  21. Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in β-cells of the Rats pancreas. Physiol Res 50:536–546Google Scholar
  22. The WHO Expert Committee on Diabetes mellitus (1980) Technical reports series 646. World Health Organization, GenevaGoogle Scholar
  23. Trease GE, Evans WC (1983) P12 ed. Balliere Tindal, London, pp 612Google Scholar
  24. Trease GE, Evans WC (2002) Pharmacognosy, 15th edn. W.B. Saunders, London, pp 58–302Google Scholar
  25. Zhang BB, Moller DE (2000) A new approach in the treatment of type 2 diabetes mellitus. Curr Opin Chem Biol 4:461–467PubMedCrossRefGoogle Scholar
  26. Zheng J, He J, Ji B, Li Y, Zhang X (2007) Antihyperglycemic effects of Platycodon grandiflorum (Jacq.) A. DC extract on streptozotocin-induced diabetic mice. Plant Foods Human Nutr 62:7–11CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2010

Authors and Affiliations

  • Ifeanyi Innocent Madubunyi
    • 1
  • Samuel Okwudili Onoja
    • 1
  • Isaac Uzoma Asuzu
    • 1
  1. 1.Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary MedicineUniversity of NigeriaNsukkaNigeria

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