Skip to main content
SpringerLink
Log in

Determination of snake-necked turtle Hydromedusa tectifera (Cope, 1870) (Testudines: Chelidae) plasma protein concentrations by refractometry and the biuret method

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

The purpose of this study was to evaluate the accuracy of hand-held refractometer in determining plasma protein concentrations in South American snake-necked turtle (Hydromedusa tectifera) as compared with the standard biuret method. The results indicated that plasma protein values may be accurately determined in snake-necked turtle with a hand-held refractometer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andreasen CB, Latimer KS, Kircher IM, Brown J (1989) Determination of chicken and turkey plasma and serum protein concentrations by refractometry and the biuret method. Avian Dis 33:93–96

    Article  PubMed  CAS  Google Scholar 

  • Bolten AB, Bjorndal KA (1992) Blood profiles for a wild population of green turtles (Chelonia mydas) in the Southern Bahamas: size-specific and sex-specific relationships. J Wildl Dis 28(3):407–413

    PubMed  CAS  Google Scholar 

  • Campbell TW (2006) Clinical pathology of reptiles. In: Mader DR (ed) Reptile medicine and surgery, 2nd edn. Saunders/Elsevier, St. Louis, pp 453–470

    Chapter  Google Scholar 

  • Eckersall PD (2008) Proteins, proteomics and the dysproteinemias. In: Kaneko JJ, Harvey JW, Bruss ML (eds) Clinical biochemistry of domestic animals, 6th edn. Academic, Burlington, pp 117–155

    Chapter  Google Scholar 

  • Ernst CH, Barbour RW (1989) Turtles of the world. Smithsonian Institution Press, Washington

    Google Scholar 

  • Fagundes CK, Bager A (2007) Ecologia reprodutiva de Hydromedusa tectifera (Testudines: Chelidae) no sul do Brasil. Biota Neotropica 7(2):179–184

    Article  Google Scholar 

  • George JW (2001) The usefulness and limitations of hand-held refractometers in veterinary laboratory medicine: an historical and technical review. Vet Clin Pathol 30(4):201–210

    Article  PubMed  Google Scholar 

  • Jacobson ER (1993) Blood collection techniques in reptiles: laboratory investigations. In: Fowler ME (ed) Zoo and animal medicine—current therapy 3. W B Saunders, Philadelphia, pp 144–152

    Google Scholar 

  • Kaneko JJ, Harvey JW, Bruss ML (2008) Clinical biochemistry of domestic animals, 6th edn. Academic, Burlington

    Google Scholar 

  • Lumeij JT, Bruijne JJ (1985) Evaluation of the refractometric method for the determination of total protein in avian plasma or serum. Avian Pathol 14:441–444

    Article  PubMed  CAS  Google Scholar 

  • Lumeij JT (2008) Avian clinical biochemistry. In: Kaneko JJ, Harvey JW, Bruss ML (eds) Clinical biochemistry of domestic animals, 6th edn. Academic, Burlington, pp 839–872

    Chapter  Google Scholar 

  • Schmidt EMS, Paulillo AC, Locatelli-Dittrich R, Hoppe EGL, Bertoli RS (2008) Determination of ring-necked pheasant (Phasianus colchicus) serum protein concentration by refractometry and the biuret method. International Journal of Poultry Science 7(7):672–673

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Veterinary Clinics Department, Veterinary Medicine Faculty, FMVZ/Unesp, Distrito Rubião Junior, s/n, CEP 18618-970, Botucatu, São Paulo, Brazil

    Elizabeth Moreira dos Santos Schmidt

  2. Veterinary Medicine Department, UFPR, Rua dos Funcionários, 1540, CEP 80035-050, Curitiba, Paraná, Brazil

    Rogério Ribas Lange

  3. Veterinary Pathology Department, FCAV/Unesp, Via de Acesso Prof Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil

    Antonio Carlos Paulillo

Authors
  1. Elizabeth Moreira dos Santos Schmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rogério Ribas Lange
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Antonio Carlos Paulillo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Elizabeth Moreira dos Santos Schmidt.

Rights and permissions

Reprints and permissions

About this article

Cite this article

dos Santos Schmidt, E.M., Lange, R.R. & Paulillo, A.C. Determination of snake-necked turtle Hydromedusa tectifera (Cope, 1870) (Testudines: Chelidae) plasma protein concentrations by refractometry and the biuret method. Comp Clin Pathol 20, 487–489 (2011). https://doi.org/10.1007/s00580-010-1024-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-010-1024-3

Keywords

Search

Navigation