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Effects of different dietary prebiotic inulin levels on blood serum enzymes, hematologic, and biochemical parameters of great sturgeon (Huso huso) juveniles

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Abstract

An 8-week growth trial was carried out in freshwater fiberglass tanks to investigate the effects of different dietary prebiotic inulin levels on hematologic and biochemical parameters and some blood serum enzymes in juvenile great sturgeon (Huso huso). Four experimental diets were formulated to contain different prebiotic inulin levels (0.0%, 1.0%, 2.0%, and 3.0%, respectively). Triplicate groups of fish (50 fish per tank with an average body weight of 16.14 ± 0.38 g) were assigned to each diet. Blood samples were taken from the caudal vein of 60 apparently healthy fish at the end of the trial. No significant differences were observed in serum enzymes between the treatment groups (P > 0.05). The results show that with the increase in supplementation level of inulin, the mean values of alkaline phosphatase decreased but the mean value of other enzymes increased. white blood cell count increased significantly in group treated with 1% inulin compared with the other groups (P < 0.05). No significant difference between the treatment groups was observed in some hematologic and biochemical parameters such as red blood cell count, mean corpuscular hemoglobin (MCH), and glucose (P > 0.05). With the increase supplementation level of inulin, the mean values of cholesterol, MCH, and MCH concentration increased, but the mean value of glucose, white blood cell count, red blood cell count, hematocrit, hemoglobin, and mean corpuscular volume decreased.

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References

  • Affonso EG, Polez VLP, Correa CF, Mazoa AF, Araujo MRR, Moraes G (2002) Blood parameters and metabolites in teleost fish Colossoma macropomum exposed to sulfide or hypoxia. Comp Biochem Physiol C 33:375–382

    Google Scholar 

  • Baker DW, Wood AM, Litvak MK, Kieffer JD (2005) Haematology of juvenile Acipenser oxyrinchus and Acipenser brevirostrum at rest and following forced activity. J Fish Biol 66:208–221

    Article  Google Scholar 

  • Ballarin L, Dalloro M, Bertotto D, Libertini A, Francescon A, Barbaro A (2004) Haematological parameters in Umbriana cirrosa (Teleostei, Scianidae): a comparison between diploid and triploid specimen. Comp Biochem Physiol A 183:45–51

    Google Scholar 

  • Blaxhall PC, Daisley KW (1973) Routine hematological methods for use with fish blood. J Fish Biol 5:771–781

    Article  Google Scholar 

  • Findeis EK (1997) Osteology and phylogenetic relationships of recent sturgeons. In: Birstein VJ, Waldman JR, Bemis WE (eds) Sturgeon. biodiversity and conservation. Kluwer Academic Publishers, Dordrecht, Germany, pp 73–106

    Google Scholar 

  • Gibson GR, Probert HM, Van Loo J, Rastall AR, Roberfroid M (2004) Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutr Res Rev 17:259–275

    Article  PubMed  CAS  Google Scholar 

  • Jalali MA, Ahmadifar E, Sudagar M, Azari Takami GH (2009) Growth efficiency, body composition, survival and haematological changes in great sturgeon (Huso huso Linnaeus, 1758) juveniles fed diets supplemented with different levels of Ergosan. Aquac Res. doi:10.1111/j.1365-2109.2009.02166.x

    Google Scholar 

  • Klontz GW (1994) Fish hematology. In: Stolen JS, Fletcher TC, Rowley AF, Kelikoff TC, Kaattari SL, Smith SA (eds) Techniques in Fish Immunology, vol. 3. SOS Publications, Fair Haven, NJ, pp 121–132

    Google Scholar 

  • Racicot JG, Gaudet M, leray C (1975) Blood and liver enzymes in rainbow trout (Salmo gairdneri) with emphasis on their diagnostic use: study of CCl4 toxicity and a case of Aeromonas infection. J Fish Biol 7:825–835

    Article  CAS  Google Scholar 

  • Rehulka J (2002) Aeromonas causes server skin lesions in rainbow trout (Oncorhynchus mykiss): clinical pathology, hematology and biochemistry. Acta Vet BRNO 71:351–360

    Article  Google Scholar 

  • Rehulka J (2003) Hematological analysis in rainbow trout (Oncorhynchus mykiss) affected by viral haemrrhagic septicaemia (VHS). Dis Aquat Org 56:185–193

    Article  PubMed  CAS  Google Scholar 

  • Rowley AF (1990) Collection, separation and identification of fish leukocytes. In: van Muiswinkel WB (ed) Techniques in fish immunology—I. SOS Publications, Fair Haven, NJ, pp 113–136

    Google Scholar 

  • Seiverd CE (1964) Hematology for medical technologists. Lea and Febiger, Philadelphia, PA, p 946

    Google Scholar 

  • Shakoori AR, Iqbal MJ, Mughal AL (1996) Effect of sublethal doses of fenvalerate (a synthetic pyrethroid) administred continuousely for four weeks on the blood, liver and muscles of a freshwater fish (Ctenophayngodon idella). Bull Environ Contam Toxical 57:487–494

    Article  CAS  Google Scholar 

  • Shalaby A (2005) The opposing effect of ascorbic acid (Vitamin C) on Ochratoxin toxicity in Nile tilapia, Oreochromis niloticus. 6th Intern. Symp. Tilapia in Aquac. Philipin, pp 150–157

  • Snieszko SF (1960) Microhaematocrit as a tool in fisheries management. Special Scientific Report—Fisheries. No.314.U.S. Department of the Interior. Fish and Fisheries Wildlife Washington, DC

  • Stoskopf MK (1993a) Fish medicine. WB Saunders Company, Philadelphia, PA, p 882

    Google Scholar 

  • Stoskopf MA (1993b) Fish medicine. WB Sounders, Philadelphia, PA, 882 pp

    Google Scholar 

  • Williams RW, Warner MC (1976) Some observation on the stained blood cellular elements of Ictalurus punctatus. J fish boil 9:491–497

    Article  Google Scholar 

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Acknowledgments

This work was carried out in Shaheed Marjani sturgeon fish breeding and rearing center (Gorgan, Iran). We express our tanks to this center expert.

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Correspondence to Asad Mohammadi Zarejabad.

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Ahmdifar, E., Akrami, R., Ghelichi, A. et al. Effects of different dietary prebiotic inulin levels on blood serum enzymes, hematologic, and biochemical parameters of great sturgeon (Huso huso) juveniles. Comp Clin Pathol 20, 447–451 (2011). https://doi.org/10.1007/s00580-010-1017-2

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