Abstract
In order to establish haematological reference intervals in the genus Tupinambis, blood samples were taken from the ventral coccygeal vein from 100 specimens of Tupinambis merianae and Tupinambis rufescens from Argentina. The samples were used to determine the haematocrit values, red blood cell count, total leukocyte and differential leukocyte count, thrombocyte count, mean corpuscular volume, haemoglobin concentration, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration and these were compared between both species. Statistically significant differences were observed as functions of the species (p > 0.001); T. merianae appeared to have higher red blood cell counts, haematocrit and haemoglobin than T. rufescens. Conversely, T. rufescens showed statistically high values in leukocyte and thrombocyte counts compared to the other species of the genus.
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References
Acuña M (1975) Hematologia normal de Iguana iguana. I. Aspectos citomorfologicos y comparaciones intraespecificas. Caribb J Sci 15(1–2):71–78
Alberio SO, Diniz AJ, Silva EO, De Souza W, Da Matta RA (2005) Cytochemical and functional characterization of blood and inflammatory cells from the lizard Ameiva ameiva. Tissue Cell 37:193–202
Andrade D, Brito SP, Toledo LF, Abe AS (2004) Seasonal changes in blood oxygen transport and acid-base status in the tegu lizard Tupinambis merianae. Resp Phys & Neurobiology 140:197–208
Blofield BA, Short L, Samour HJ, Ball D, Hawkey CM (1992) Phylogenetic and haematological implications of differences in the morphology of the heterophils nucleus in reptiles. J Zool London 226:539–549
Carvalho RL, Antoniazzi MM, Jared C, Silva AMJ, Santos AA, Egami MI (2003) Comparative structure of blood heterophils in Tupinambis merianae (Reptilia) and Rhinoptynix clamator (Ave). Acta Microscopica 12(B):585–586
Carvalho RL, Antoniazzi MM, Jared C, Silva AMJ, Santos AA, Egami MI (2006) Morphological, cytochemical, and ultrastructural observations on the blood cells of the reptile Tupinambis merianae (Squamata) Comp. Clin Path 15(3):169–174
Ceballos de Bruno S, Giamello de Gambulli E (1987) Contribución al estudio eritrocitario de algunos reptiles de La Pampa. Primeras Jornadas Nacionales de Fauna Silvestre 2:309–328
Ceballos de Bruno S (1995) Algunos parámetros hematológicos en Liolaemus wiegmanii (Sauria, Tropiduridae). Número de eritrocitos, número de leucocitos y fórmula leucocitaria. Morfología de células sanguíneas y de médula ósea. Cuadernos de Herpetología 9(1):51–56
Duguy R (1982) Number of blood cells and their variations. In: Gans C (ed) Biology of the Reptilia (III). Academic, New York, pp 155–188
Fisse A, Draud M, Raphael B, Melkonian K (2004) Different leukocyte count of critically endangered Grand Cayman Blue iguanas (Cyclura nubila lewisii). J Herp Med Surgery 14(4):19–21
Gorman GC, Dessauer HC (1965) Haemoglobin and transferrin electrophoresis and relationship of island populations of Anolis lizards. Science 150:1454–1455
Gorman GC, Dessauer HC (1966) The relationship of Anolis of the Roquet species group (Sauria–Iguanidae) I. Electrophoretic comparison of blood proteins. Comp Biochem Physdiol 19:845–853
Hawkey CM, Dennet TB (1994) A colour atlas of comparative hematology. Wolfee Medical Publications Ltd, Ipswich, England
Knotkova Z, Knotek Z, Trmkova S, Miculkova P (2006) Blood profile in green iguana after short-term anaesthesia with propofol. Vet Med 51(10):491–496
Martinez Silvestre A, Rodriguez Dominguez MA, Mateo JA, Pastor J, Marco I, Lavin S, Cuenca R (2004) Comparative haematology and blood chemistry of endangered lizards (Gallotia species) in the Canary Island. Vet Rec 155:266–269
Mayer J, Knoll J, Innis C, Mitchell MA (2005) Characterizing the hematological and plasma chemistry profiles of captive Chinese water dragon Physignathus concincinnus. J Herp Med Surg 15(3):193–202
Mermer A, Rkuye U (1999) Investigation on the blood cell counts of Chalcides ocellatus (Sauria: Scincidae) population in Anatolia. J Zool 23(1):55–58
Natt MP, Herrick CA (1952) A new diluent for counting the erythrocytes and leukocytes of the chicken. Poult Scien 31:735–738
Peña Rocha H (1949) Contribuciones a la morfología comparada de la fauna chilena. Estudios hematológicos de las especies Lioalemus nigromaculatus y Liolaemus pictus. Bol Soc Biol Concepción 13:133–146
Pienaar UV (1962) Hematology of some South African reptiles. University of Witwatersrand Press, Johannesburg (RSA)
Pollack Velásquez L, Zelada Estraver W, Zavala de la Cruz F, Alva MA, Tirado Pinedo A (2006) Valores hematológicos de Dicrodon guttulatum y Dicrodon heterolepis (Reptilia: Teiidae). Revista Ciencia y Tecnología 3: 93–101
Rosenfeld G (1947) Corante pancromático para hematologia e citologia humana. Nova combinação dos componentes do May-Grünwald e do Giemsa num só corante de emprego rápido. Mem Inst Butantan 20:328–334
Troiano JC, Gould EG, Gould I (2008) Haematological reference intervals in Argentine lizard Tupinambis merianae (Sauria–Teiidae). Comp Clin Path 17:93–97
Acknowledgements
Wiener Laboratorios (Rosario, República Argentina) and Vetanco Laboratorios (Buenos Aires, Argentina)
Mr. Adriano Carfi, Pablo and Mariano Llaver from Cañuelas Breeding Center
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Troiano, J.C., Gould, E.G. Interspecific differences in the haematological reference intervals in Tupinambis genus from Argentina (Sauria–Teiidae). Comp Clin Pathol 20, 309–312 (2011). https://doi.org/10.1007/s00580-010-0992-7
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DOI: https://doi.org/10.1007/s00580-010-0992-7