Abstract
This study aimed to detect brain and red blood cell acetylcholinesterase activity (AChE and RBC-AChE, respectively) of rats treated with vincristine sulphate and different doses of nandrolone decanoate. Thirty rats were divided into six groups (n = 5). The treatments were applied once a week for 2 weeks. Sample collection was performed in the third week. The groups were divided in: G1, physiologic solution (PS) (first and second week); G2, vincristine sulphate (4 mg/m2) in the first week and PS in the second week; G3, PS in the first week and nandrolone decanoate (1.8 mg/kg−1) in the second week; G4, PS in the first week and nandrolone decanoate (10 mg/kg−1) in the second week; G5, vincristine sulphate (4 mg/m2) in the first week and nandrolone decanoate (1.8 mg/kg−1) in the second week; and G6, vincristine sulphate (4 mg/m2) in the first week and nandrolone decanoate (10 mg/kg−1) in the second week. The isolated use of nandrolone decanoate and its use in association with vincristine sulphate altered brain AChE and RBC-AChE activity. These results suggest that there is interference in cholinergic neurotransmission, which could cause an alteration of its neurotransmitter, as well as a low or high stimulation of post-synaptic receptors. The serum RBC-AChE activity results presented in this study are similar to those exhibited by brain tissue.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bagchus WM, Smeets JMW, Verheul HA, De Jager-Van Der Veen SM, Port A, Geurts TB (2005) Pharmacokinetic evaluation of three different intramuscular doses of nandrolone decanoate: analysis of serum and urine samples in healthy men. J Clin Endocrinol Metab 90:2624–2630
Basaria S, Wahlstrom JT, Dobs AS (2001) Anabolic-androgenic steroid therapy in the treatment of chronic diseases. J Clin Endocrinol Metab 86:5108–5117
Bermudez M, Fuster JL, Llinares E, Galera A, Gonzalez C (2005) Itraconazole-related increased vincristine neurotoxicity: case report and review of literature. J Pediatr Hematol Oncol 27:389–392
Bernhardi RV, Alarcón R, Mezzano D, Fuentes P, Inestrosa NC (2005) Blood cells cholinesterase activity in early Alzheimer's disease and vascular dementia. Dement Geriatr Cogn Disord 19:204–212
Cave TA, Norman P, Mellor D (2007) Cytotoxic drug use in treatment of dogs and cats with cancer by UK veterinary practices (2003 to 2004). J Small Anim Pract 48:371–377
Cooper JR, Bloom FE, Roth RH (1991) Acetylcholine. In: Cooper JR, Bloom FE, Roth RH (eds) The biochemical basis of neuropharmacology, 6th edn. Oxford University Press, Oxford, pp 190–213
Cuddon PA (2002) Acquired canine peripheral neuropathies. Vet Clin North Am Small Anim Pract 32:207–249
Ellman GL, Courtney KD, Andres VJ, Feather-Stone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 21:88–95
Gadjeva V, Dimov A, Georgieva N (2008) Influence of therapy on the antioxidant status in patients with melanoma. J Clin Pharm Ther 33:179–185
Hughes K, Scase TJ, Ward C, Polton GA (2009) Vincristine overdose in a cat: clinical management, use of calcium folinate, and pathological lesions. J Feline Med Surg 11:322–325
Jameson RR, Seidler FJ, Slotkin TA (2007) Nonenzymatic functions of acetylcholinesterase splice variants in the developmental neurotoxicity of organophosphates: chlorpyrifos, chlorpyrifos oxon, and diazinon. Environ Health Perspect 115:65–70
Marcel V, Palacios LG, Pertuy C, Masson P, Fournier D (1998) Two invertebrate acetylcholinesterases show activation followed by inhibition with substrate concentration. Biochem J 329:329–334
Massoulié J, Anselmet A, Bon S, Krejci E, Legay C, Morel N, Simon S (1999) The polymorphism of acetylcholinesterase: post-translational processing, quartenary associations and localization. Chem Biol Interact 119–120:29–42
McClelland RB (1963) Cyclophosphamide therapy in lymphoma of the dog. Cornell Vet 53:319–322
McGehee DS, Krasowski MD, Fung DL, Wilson B, Gronert GA, Moss J (2000) Cholinesterase inhibition by potato glycoalkaloids slows mivacurium metabolism. Anesthesiology 93:510–511
McKnight JA (2003) Principles of Chemotherapy. Clin Tech Small Anim Pract 18:67–72
Mirkhani H, Golbahar J, Shokri S (2005) Chronic administration of nandrolone decanoate does not increase the plasma homocysteine level of male rats. Basic Clin Pharmacol Toxicol 97:214–217
Morley JE, Thomas DR, Wilson MMG (2006) Cachexia: pathophisiology and clinical relevance. Am J Clin Nutr 83:735–743
Nak D, Nak Y, Cangul IT, Tuna B (2005) A clinical-pathological study on the effect of vincristine on transmissible venereal tumour in dogs. J Vet Med 52:366–370
Norris AM, Withrow SJ (1984) A review of cancer chemotherapy for pet animals. Can Vet J 25:153–157
Perez RR, Silva MAML, Varzim FLSB, Oliveira SB, Hucke EETS (2005) Nandrolone decanoate (Deca Durabolin) effect on hematologic parameters and total plasma protein of rats (Rattus rattus) with depressed bone marrow induced by vincristine (Oncovin). Ciencia Rural 35:589–595
Poncé F, Magnol JP, Ledieu D, Marchal T, Turinelli V, Chalvet-Monfray K, Fournel-Fleury C (2004) Prognostic significance of morphological subtypes in canine malignant lymphomas during chemotherapy. Vet J 167:158–166
Rocha JBT, Emanuelli T, Pereira ME (1993) Effects of early undernutrition on kinetic parameters of brain acetylcholinesterase from adult rats. Acta Neurobiol Exp 53:431–437
Rodaski S, Nardi AB (2006) Quimioterapia antineoplásica em cães e gatos (Anticancer chemotherapy for dogs and cats), 2nd edn. Bio Editora, Curitiba, 308p
Rogers KS, Walker MA, Dillon HB (1998) Transmissible venereal tumor: a retrospective study of 29 cases. J Am Anim Hosp Assoc 34:463–470
Saitoh T, Morimoto K, Kumagai T, Tsuboi I, Aikawa S, Horie T (1999) Comparision of erythropoietic response to androgen in young and old senescence accelerated rats. Mech Ageing Dev 109:125–139
Seaman RL, Patton CS (2003) Treatment of renal nephroblastoma in an adult dog. J Am Anim Hosp Assoc 39:76–79
Thiermann H, Szinicz L, Eyer P, Zilker T, Worek F (2005) Correlation between red blood cell acetylcholinesterase activity and neuromuscular transmission in organophosphate poisoning. Chem Biol Interact 157–158:345–347
Villalobos A (2006) Dealing with chemotherapy extravasations: a new technique. J Am Anim Hosp Assoc 42:321–325
Whittaker JA, Parry DH, Bunch C, Weatherall DJ (1973) Coma associated with vincristine therapy. Br Med J 4:335–337
Worek F, Mast U, Kinderlen D, Diepold C, Eyer P (1999) Improved determination of acetylcholinesterase activity in human whole blood. Clin Chim Acta 288:73–90
Zimmerman G, Soreq H (2006) Termination and beyond: acetylcholinesterase as a modulator of synaptic transmission. Cell Tissue Res 326:655–669
Acknowledgements
This research was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Martins, D.B., Mazzanti, C.M., Spanevello, R. et al. Cholinergic system of rats treated with vincristine sulphate and nandrolone decanoate. Comp Clin Pathol 20, 33–37 (2011). https://doi.org/10.1007/s00580-009-0948-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00580-009-0948-y