Abstract
The present study investigated the effects of exogenous cyclic GMP (cGMP) and cyclic AMP (cAMP) on kinematic parameters of epididymal ram sperm in vitro. After incubation of epididymal sperm samples for 45 and 90 min in the presence of 8-Br-cAMP (0.1, 1, and 5 mM) and 8-Br-cGMP (0.05, 0.5, and 5 mM), motion parameters were evaluated by computer-aided sperm analysis. At 0.1 mM concentration of 8-Br-cAMP, there was a significant increase in the fast progressive motility (at 45 min), nonprogressive motility (at 90 min), amplitude of lateral head displacement (ALH; at 45 min), curvilinear velocity (VCL; at 90 min), and mean angle of deviation (MAD; at 90 min) of sperm while a decrease in the immotility and beat cross frequency (BCF; at 45 and 90 min) in cAMP-treated groups was observed. At 1 mM of 8-Br-cAMP, there was a significant increase in the fast progressive motility (at 45 min), nonprogressive motility (at 90 min), VCL (at 45 and 90 min), straight line velocity (VSL), average path velocity (VAP), MAD, ALH, linearity (LIN), and straightness (STR; at 45 min) of sperm but decreasing immotility, BCF (at 45 and 90 min) and wobble (at 90 min) in cAMP-treated groups compared to controls. At 5 mM of 8-Br-cAMP, a significant elevation in the immotility and BCF of sperm was observed while reductions in the fast progressive motility, VCL,VSL,VAP, MAD, ALH, STR, and LIN was noted in the cAMP-treated groups at 45 and 90 min of incubation. The effects of 8-Br-cGMP on sperm motility were not significant. In conclusion, low concentrations of exogenous cAMP but no cGMP improves the motion parameters of ram epididymal sperm.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adeoya-Osiguwa SA, Fraser LR (2002) Capacitation state-dependent changes in adenosine receptors and their regulation of adenylyl cyclase/cAMP. Mol Reprod Dev 63:245–255
Baxendale RW, Fraser LR (2003) Evidence for multiple distinctly localized adenylyl cyclase isoforms in mammalian spermatozoa. Mol Reprod Dev 66:181–189
Blash S, Melican D, Gavin W (2000) Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology 54:899–905
Brautigan DL, Pinault FM (1991) Activation of membrane protein-tyrosine phosphatase involving cAMP-and Ca2+/phospholipid-dependent protein kinases. Proceedings of the National Academy of Sciences 88:6696–6700
Dimitriadis F, Giannakis D, Pardalidis N, Zikopoulos K, Paraskevaidis E, Giotitsas N, Kalaboki V, Tsounapi P, Baltogiannis D, Georgiou I (2008) Effects of phosphodiesterase 5 inhibitors on sperm parameters and fertilizing capacity. Asian J Androl 10:115–133
Esposito G, Jaiswal BS, Xie F, Krajnc-Franken MAM, Robben T, Strik AM, Kuil C, Philipsen RLA, van Duin M, Conti M (2004) Mice deficient for soluble adenylyl cyclase are infertile because of a severe sperm-motility defect. Proceedings of the National Academy of Sciences 101:2993–2998
Fisch JD, Behr B, Conti M (1998) Enhancement of motility and acrosome reaction in human spermatozoa: differential activation by type-specific phosphodiesterase inhibitors. Hum Reprod 13:1248–1254
Galantino-Homer HL, Visconti PE, Kopf GS (1997) Regulation of protein tyrosine phosphorylation during bovine sperm capacitation by a cyclic adenosine 3′5′-monophosphate-dependent pathway. Biol Reprod 56:707–719
Gautier-Courteille C, Salanova M, Conti M (1998) The olfactory adenylyl cyclase III is expressed in rat germ cells during spermiogenesis 1. Endocrinology 139:2588–2599
Hurley JH (1998) The adenylyl and guanylyl cyclase superfamily. Curr opin struc biol 8:770–777
Leclerc P, De Lamirande E, Gagnon C (1996) Cyclic adenosine 3′, 5′monophosphate-dependent regulation of protein tyrosine phosphorylation in relation to human sperm capacitation and motility. Biol Reprod 55:684–692
MacLeod JM, Paupard MC, Orr AG (1991) Flagellar-associated cAMP-dependent protein kinases in mammalian sperm. Comparative spermatology 20 years after. 397–401
Middendorff R, Muller D, Wichers S, Holstein AF, Davidoff MS (1997) Evidence for production and functional activity of nitric oxide in seminiferous tubules and blood vessels of the human testis 1. Vol. 82, pp. 4154–4161. Endocrine Soc
Revelli A, Soldati G, Costamagna C, Pellerey O, Aldieri E, Massobrio M, Bosia A, Ghigo D (1999) Follicular fluid proteins stimulate nitric oxide (NO) synthesis in human sperm: a possible role for NO in acrosomal reaction. J cell physiol 178:58–92
Revelli A, Ghigo D, Moffa F, Massobrio M, Tur-Kaspa I (2002) Guanylate cyclase activity and sperm function. Endocr Rev 23:484–494
Taussig R, Gilman AG (1995) Mammalian membrane-bound adenylyl cyclases. J Biol Chem 270:1–4
Verstegen J, Iguer-Ouada M, Onclin K (2002) Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 57:149–179
Visconti PE, Moore GD, Bailey JL, Leclerc P, Connors SA, Pan D, Olds-Clarke P, Kopf GS (1995) Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway. Vol. 121, pp. 1139–1150
Yanagimachi R (2008) Fertility of mammalian spermatozoa: its development and relativity. Zygote 2:371–372
Zhang H, Zheng RL (1996) Possible role of nitric oxide on fertile and asthenozoospermic infertile human sperm functions. Free Radical Res 25:347–354
Acknowledgments
This work had been supported by the funds granted by the Applied Research Centre, Vice Chancellor for Research of Islamic Azad University, Karaj branch.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Goodarzi, A.K., Hassanpour, H. & Teshfam, M. Different effects of cGMP and cAMP analogs on kinematic parameters of ram epididymal sperm in vitro. Comp Clin Pathol 19, 559–563 (2010). https://doi.org/10.1007/s00580-009-0922-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00580-009-0922-8