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The acute and subchronic toxicity studies of aqueous leaf and stem bark extract of Costus afer ker (Zingiberaceae) in mice

Abstract

This work investigated the acute and subchronic toxicity profile of aqeous stem bark and leaf extract of Costus afer ker (Zingiberaceae). This plant is used extensively for food, economic, and medicinal purposes in Nigeria. Acute oral toxicity studies in male and female mice did not produce any sign of acute toxicity or death even at the dose of 4,000 mg/kg. Thirty-six albnino Wistar mice of varied sexes weighing between 28 and 35 g were divided into four groups. Groups A, B, and C were given different concentrations of the extract at 20, 13.33, and 6.67 mg/ml, respectively in water. Group D served as the control and was given only water. The experiment lasted for a period of 56 days. The packed cell volume, hemoglobin concentration, and the total red blood cell count decreased significantly (p < 0.05) in comparison with the control. Serum biochemistry showed no significant changes in the activities of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase when compared with the control. The histopathological studies revealed no significant lesions in the heart, kidney, and liver. There was also no significant chages in weight between the experimental animals and the control.

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References

  • Anaga AO, Njoku CJ, Ekejiuba ES, Esiaka MN, Asuzu IU (2004) Investigations of the methanolic leaf extract of Costus afer ker for pharmacological activities in vitro and in vivo. Phytomedicine 11:242–244

    Article  CAS  PubMed  Google Scholar 

  • Bessey OA, Lowry OH, Brock MJ (1964) Colorimetric method for determination of Alkaline phosphatase. Biol Chem 37:32–324

    Google Scholar 

  • Bidhe RM, Ghosh S (2004) Acute and Sub chronic (28-day) oral toxicity study in rats fed with novel surfactants. AAPS Pharm Sci 6(2):14

    Article  Google Scholar 

  • Coles EH (1968) Veterinary clinical pathlology. WB. Saunders Co, Philadelphia, pp 15–17

    Google Scholar 

  • Ihedioha JI, Chineme CN (2004) Fundamentals of systemic veterinary pathology vol 1. Great AP Express Publishers limited, Nsukka, pp 111–120

    Google Scholar 

  • Iwu MM (1982) Perspectives of Igbo tribal ethnomedicine. Cecta Press, Enugu, Nigeria, pp 4–5

    Google Scholar 

  • Iwu MM (1983) Traditional Igbo Medicine. Institute of African Studies, University of Nigeria, Nsukka, pp 122–144

    Google Scholar 

  • Iwu MM (1993) Handbook of African medicinal plants. CRC Press, London, pp 161–162

    Google Scholar 

  • Lindsey JR (1991) Infectious diseases of mice and rats. National Research Council, National Academy Press, Washington DC, pp 192–194

    Google Scholar 

  • Meyer DJ, Coles EH, Rich LJ (1992) Veterinary laboratory medicine—interpretation and diagnosis. WB Saunders Company, Philadelphia

    Google Scholar 

  • Oliver B (1980) Medicinal plants in Nigeria. Nigeria College of Arts, Science and Technology, Ibadan, pp 1–23

    Google Scholar 

  • Reitman S, Frankel S (1957) A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–62

    CAS  PubMed  Google Scholar 

  • Saponins (2006) Herbs 2000.com-Number one source of traditional and nutritional healthcare. http://www.herbs2000.com/saponins

  • Schalm OW, Jain NC, Carroll EJ (1975) Veterinary hematology, 3rd edn. Lea and Febiger, Philadelphia, p 45

    Google Scholar 

  • Shetty SN, Anika SM (1982) Laboratory manual of pharmacology and toxicology. Fourth Dimension Publishing co. Ltd, Enugu, pp 30–37

    Google Scholar 

Download references

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Correspondence to Samuel Chukwuneke Udem.

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Udem, S.C., Ezeasor, C.K. The acute and subchronic toxicity studies of aqueous leaf and stem bark extract of Costus afer ker (Zingiberaceae) in mice. Comp Clin Pathol 19, 75–80 (2010). https://doi.org/10.1007/s00580-009-0906-8

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  • DOI: https://doi.org/10.1007/s00580-009-0906-8

Keywords

  • Costus afer
  • Subchronic toxicity
  • Transaminases
  • Hematology
  • Histopathology