Abstract
The effects of sterilization time, sterilizing agents (ethanol, Chloramine T, calcium hypochlorite) and antibiotics (streptomycin and gentamycin) on Glomus mosseae (BEG 12) sporocarp germination and contamination were evaluated. Incubation for 10 s in 96 % ethanol, followed by 10 min in a solution of 2% Chloramine T, 0.02% streptomycin, 0.01% gentamycin and Tween 20, and then 6 min in 6% calcium hypochlorite greatly reduced fungal and bacterial contamination from sporocarps and caused little change in germination rate in water agar medium.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Accepted: 4 March 1999
Rights and permissions
About this article
Cite this article
Budi, S., Gianinazzi, S. & Blal, B. Surface-sterilization of Glomus mosseae sporocarps for studying endomycorrhization in vitro. Mycorrhiza 9, 65–68 (1999). https://doi.org/10.1007/s005720050001
Issue Date:
DOI: https://doi.org/10.1007/s005720050001