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Mycorrhiza

, Volume 11, Issue 2, pp 59–66 | Cite as

Cultivation of mushrooms of edible ectomycorrhizal fungi associated with Pinus densiflora by in vitro mycorrhizal synthesis

I. Primordium and basidiocarp formation in open-pot culture
  • A. Yamada
  • Takeo Ogura
  • Masatake Ohmasa
Original Paper

Abstract 

Edible mushroom fungi in the genera Lyophyllum, Tricholoma, Leucopaxillus, Suillus, Rhizopogon, Lactarius, and Morchella were tested for mycorrhization with Pinus densiflora in vitro. Most of the tested fungi in the genera Lyophyllum, Tricholoma, Suillus, Rhizopogon, and Lactarius formed ectomycorrhizas 2–4 months after fungal inoculation. Mycorrhizal seedlings were then acclimatized in open-pot soil under growth-chamber conditions. Almost all mycorrhizal seedlings sustained their symbiont and developed new mycorrhizas for 8–9 months after transplantation. Under these conditions, more than half of the tested species formed primordia and Tricholoma flavovirens, Rhizopogon rubescens, and Lactarius akahatsu developed basidiocarps with young host plants.

Keywords Edible mushrooms Ectomycorrhizas Cultivation In vitro synthesis Basidiocarp 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • A. Yamada
    • 1
  • Takeo Ogura
    • 1
  • Masatake Ohmasa
    • 2
  1. 1.Ibaraki Prefectural Forestry Center, 4692 Toh, Naka machi, Naka gun, Ibaraki 311-0122, Japan e-mail: akiyosh@gipmc.shinshu-u.ac.jp Tel.: +81-265-771631, Fax: +81-265-771629JP
  2. 2.Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa mura, Kamiina gun, Nagano 399-4598, JapanJP

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