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New insights into HcPTR2A and HcPTR2B, two high-affinity peptide transporters from the ectomycorrhizal model fungus Hebeloma cylindrosporum

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Abstract

While plants mainly rely on the use of inorganic nitrogen sources like ammonium and nitrate, soil-borne microorganisms like the ectomycorrhizal fungus Hebeloma cylindrosporum can also take up soil organic N in the form of amino acids and peptides that they use as nitrogen and carbon sources. Following the previous identification and functional expression in yeast of two PTR-like peptide transporters, the present study details the functions and substrates of HcPTR2A and HcPTR2B by analysing their transport kinetics in Xenopus laevis oocytes. While both transporters mediated high-affinity di- and tripeptide transport, HcPTR2A also showed low-affinity transport of several amino acids—mostly hydrophobic ones with large side chains.

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Acknowledgements

We thank Mrs. Annie Buchwalter for English editing.

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All data and materials are available.

Funding

Our work was partially funded by grants from the Deutsche Forschungsgemeinschaft (Gottfried-Wilhelm-Leibniz; DFG WI1994/2-1 and 2-2).

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Authors and Affiliations

  1. IZMB, Transport in Ectomycorrhiza, University Bonn, 53115, Bonn, Germany

    Tobias Müller & Daniel Wipf

  2. Institute of Crop Science, Nutritional Crop Physiology, University of Hohenheim, Fruwirthstr. 20, D-70593, Stuttgart, Germany

    Benjamin Neuhäuser & Uwe Ludewig

  3. BPMP, Univ Montpellier, CNRS, INRAE, Institut Agro, Montpellier, France

    Gabriella Houdinet & Sabine D. Zimmermann

  4. Agroécologie, AgroSup Dijon, CNRS, Université de Bourgogne, INRAE, Université de Bourgogne Franche-Comté, 17 Rue Sully, 21000, Dijon, France

    Pierre Emmanuel Courty & Daniel Wipf

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  1. Tobias Müller
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Correspondence to Daniel Wipf.

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Müller, T., Neuhäuser, B., Ludewig, U. et al. New insights into HcPTR2A and HcPTR2B, two high-affinity peptide transporters from the ectomycorrhizal model fungus Hebeloma cylindrosporum. Mycorrhiza 30, 735–747 (2020). https://doi.org/10.1007/s00572-020-00983-7

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