Root colonization and arbuscular mycorrhizal fungal community composition in a genetically modified maize, its non-modified isoline, and a landrace

Londoño, Diana Marcela Morales; Meyer, Edenilson; da Silva, Kelly Justin; Hernández, Anabel González; de Armas, Rafael Dutra; Soares, Luis Macedo; Stürmer, Sidney Luiz; Nodari, Rubens Onofre; Soares, Cláudio Roberto Fonsêca Sousa; Lovato, Paulo Emilio

doi:10.1007/s00572-020-00969-5

Original Article
Published: 15 June 2020

Root colonization and arbuscular mycorrhizal fungal community composition in a genetically modified maize, its non-modified isoline, and a landrace

Mycorrhiza volume 30, pages 611–621 (2020)Cite this article

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Abstract

The use of genetically modified (GM) plants has increased in recent decades, but there are uncertainties about their effects on soil microbial communities. Aiming to quantify root colonization and characterize arbuscular mycorrhizal fungi (AMF) communities associated with roots and rhizosphere soil of different maize genotypes, a field trial was carried out in Southern Brazil with three maize genotypes as follows: a GM hybrid (DKB 240 VTPRO), its non-modified isoline (DKB 240), and a landrace (Pixurum). Soil samples were collected to evaluate the occurrence of AMF during the growth of corn genotypes at sowing and V3 (vegetative), R1 (flowering), and R3 (grain formation) stages of the crop. The occurrence of AMF was determined by the morphological identification of spores, and by analyzing AMF community composition in soil and roots of maize, using PCR-DGGE. The GM genotype of maize promoted lower mycorrhizal colonization in the vegetative stage and had lower sporulation at grain development than the conventional hybrid and the landrace maize. Twenty AMF morphotypes were identified and 13 were associated with all maize genotypes. The genera Acaulospora, Glomus, and Dentiscutata had the largest numbers of species. There were no differences in AMF community composition due to maize genotypes or genetic modification, but crop phenological stages affected AMF communities associated with maize roots.

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Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES (Finance Code 001) and CNPq funded this work.

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Departamento de Engenharia Rural, Centro de Ciências Agrarias, Universidade Federal de Santa Catarina. Rodovia Admar Gonzaga, 1346, Florianópolis, Santa Catarina, CEP: 88034-001, Brazil
Diana Marcela Morales Londoño, Edenilson Meyer & Paulo Emilio Lovato
Centro Universitário Católica de Santa Catarina – Joinville, Campus Joinville. Rua Visconde de Taunay 427, Joinville, Santa Catarina, CEP 89203-005, Brazil
Kelly Justin da Silva & Rafael Dutra de Armas
Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina. Campus Reitor João David Ferreira Lima, Florianópolis, Santa Catarina, CEP 88040-900, Brazil
Anabel González Hernández & Cláudio Roberto Fonsêca Sousa Soares
Departamento de Ecologia e Zoologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina. Campus Reitor João David Ferreira Lima, Florianópolis, Santa Catarina, CEP 88040-900, Brazil
Luis Macedo Soares
Departamento de Ciencias Naturais, Centro de Ciencias Exatas e Naturais, Fundação Universidade Regional de Blumenau. Rua Antonio da Veiga, 140, Centro, Blumenau, Santa Catarina, CEP 89030-903, Brazil
Sidney Luiz Stürmer
Departamento de Fitotecnia, Centro de Ciências Agrárias Universidade Federal de Santa Catarina. Rodovia Admar Gonzaga, 1346, Bloco B, Itacorubi, Florianópolis, Santa Catarina, CEP 88040-900, Brazil
Rubens Onofre Nodari

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Diana Marcela Morales Londoño
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Edenilson Meyer
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Kelly Justin da Silva
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Anabel González Hernández
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Rafael Dutra de Armas
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Luis Macedo Soares
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Sidney Luiz Stürmer
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Rubens Onofre Nodari
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Cláudio Roberto Fonsêca Sousa Soares
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Paulo Emilio Lovato
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Contributions

D.M.M.L conceived and designed the study, performed research, analyzed data, and wrote the first version of the manuscript. EM wrote the second version of the manuscript. E.M, R.D.A, L.M.S., and C.R.F.S.S conducted research, analyzed data, and wrote parts of the manuscript. K.J.S, A.G.H, S.L.S, and R.O.N analyzed data and revised the text. P. E. L contributed to the study design and wrote the final version of the manuscript.

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Correspondence to Paulo Emilio Lovato.

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Londoño, D.M.M., Meyer, E., da Silva, K.J. et al. Root colonization and arbuscular mycorrhizal fungal community composition in a genetically modified maize, its non-modified isoline, and a landrace. Mycorrhiza 30, 611–621 (2020). https://doi.org/10.1007/s00572-020-00969-5

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Received: 02 March 2020
Accepted: 02 June 2020
Published: 15 June 2020
Issue Date: September 2020
DOI: https://doi.org/10.1007/s00572-020-00969-5

Keywords

AMF
Corn
GM crops
PCR-DGGE
Phenological stages
Zea mays L.