Soil lead pollution modifies the structure of arbuscular mycorrhizal fungal communities

Faggioli, Valeria; Menoyo, Eugenia; Geml, József; Kemppainen, Minna; Pardo, Alejandro; Salazar, M. Julieta; Becerra, Alejandra G.

doi:10.1007/s00572-019-00895-1

Original Article
Published: 23 April 2019

Soil lead pollution modifies the structure of arbuscular mycorrhizal fungal communities

Valeria Faggioli¹,
Eugenia Menoyo²,
József Geml³,
Minna Kemppainen⁴,
Alejandro Pardo⁴,
M. Julieta Salazar⁵ &
Alejandra G. Becerra⁵

Mycorrhiza volume 29, pages 363–373 (2019)Cite this article

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Abstract

The impact of lead (Pb) pollution on native communities of arbuscular mycorrhizal fungi (AMF) was assessed in soil samples from the surroundings of an abandoned Pb smelting factory. To consider the influence of host identity, bulk soil surrounding plant roots soil samples of predominant plant species (Sorghum halepense, Bidens pilosa, and Tagetes minuta) growing in Pb-polluted soils and in an uncontaminated site were selected. Molecular diversity was assessed by sequencing the 18S rDNA region with primers specific to AMF (AMV4.5NF/AMDGR) using Illumina MiSeq. A total of 115 virtual taxa (VT) of AMF were identified in this survey. Plant species did not affect AMF diversity patterns. However, soil Pb content was negatively correlated with VT richness per sample. Paraglomeraceae and Glomeraceae were the predominant families while Acaulosporaceae, Ambisporaceae, Archaeosporaceae, Claroideoglomeraceae, Diversisporaceae, and Gigasporaceae were less abundant. Acaulosporaceae and Glomeraceae were negatively affected by soil Pb, but Paraglomeraceae relative abundance increased under increasing soil Pb content. Overall, 26 indicator taxa were identified; four of them were previously reported in Pb-polluted soils (VT060; VT222; VT004; VT380); and five corresponded to cultured spores of Scutellospora castaneae (VT041), Diversispora spp. and Tricispora nevadensis (VT060), Diversispora epigaea (VT061), Glomus proliferum (VT099), and Gl. indicum (VT222). Even though AMF were present in Pb-polluted soils, community structure was strongly altered via the differential responses of taxonomic groups of AMF to Pb pollution. These taxon-specific differences in tolerance to soil Pb content should be considered for future phytoremediation strategies based on the selection and utilization of native Glomeromycota.

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Acknowledgements

Alejandra G. Becerra, Julieta Salazar, and Eugenia Menoyo are researchers of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina.

We are very grateful to the Editor in Chief and two anonymous Reviewers for helping to improve the manuscript.

Funding

This work was partially supported by the Fondo para la Investigación Científica y Técnica (FONCyT, grant number PICT 2013-049, PICT 2013-2146).

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Instituto Nacional de Tecnología Agropecuaria, EEA Marcos Juárez, Ruta 12 km 36, 2580, Marcos Juárez, Argentina
Valeria Faggioli
Grupo de Estudios Ambientales (GEA), Instituto de Matemática Aplicada San Luis (IMASL)–CONICET, Universidad Nacional de San Luis, Ejército de los Andes 950, 5700, San Luis, Argentina
Eugenia Menoyo
Biodiversity Dynamics Research Group, Naturalis Biodiversity Center, Vondellaan 55, 2332 AA, Leiden, The Netherlands
József Geml
Laboratorio de Micología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, y CONICET, Universidad Nacional de Quilmes, Roque Sáenz Peña, 352, Bernal, Provincia de Buenos Aires, Argentina
Minna Kemppainen & Alejandro Pardo
Instituto Multidisciplinario de Biología Vegetal (IMBIV)-CONICET, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Av. Vélez Sársfield, 1611, Córdoba, Argentina
M. Julieta Salazar & Alejandra G. Becerra

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Valeria Faggioli
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Faggioli, V., Menoyo, E., Geml, J. et al. Soil lead pollution modifies the structure of arbuscular mycorrhizal fungal communities. Mycorrhiza 29, 363–373 (2019). https://doi.org/10.1007/s00572-019-00895-1

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Received: 29 December 2018
Accepted: 08 April 2019
Published: 23 April 2019
Issue Date: 01 July 2019
DOI: https://doi.org/10.1007/s00572-019-00895-1

Keywords

Heavy metal
Soil pollution
18S rDNA
Arbuscular mycorrhizal fungi
Biodiversity