Abstract
Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Behnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L−1 dry weight and productivity increased from 10.7 to 95.8 mg L−1 day−1 dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization.
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Acknowledgements
This work was supported by projects 19484/PI/14 (Fundación Séneca of Region of Murcia, FEDER, Spain) and CGL2016-78946-R (AEI-FEDER, UE). F. Arenas thanks the Ministerio de Economía y Competitividad (MINECO) for financial resources from the Youth Employment Initiative (IEJ) and the European Social Fund (FSE) (PEJ-2014-A-83659). D. Chávez would like to thank the Chilean National Council for Science and Technology CONICYT 21110038 for the assigned postgraduate grant. A. Navarro-Ródenas is grateful to the University of Murcia for a postdoctoral contract.
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Fig. S1
Summary of the different experiments performed. (JPG 61 kb)
Fig. S2
Parameter profile during mycelial growth of Terfezia claveryi in bioreactor. Symbols for the parameters used: pH (short dash), mL of NaOH added (dotted) and dissolved oxygen (%DO) (solid). (DOCX 90 kb)
Table S1
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Table S2
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Arenas, F., Navarro-Ródenas, A., Chávez, D. et al. Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants. Mycorrhiza 28, 691–701 (2018). https://doi.org/10.1007/s00572-018-0867-3
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DOI: https://doi.org/10.1007/s00572-018-0867-3