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Mycorrhiza

, Volume 28, Issue 3, pp 221–233 | Cite as

Management regime is the most important factor influencing ectomycorrhizal species community in Norway spruce forests after windthrow

  • Martina Vašutová
  • Magda Edwards-Jonášová
  • Petra Veselá
  • Lenka Effenberková
  • Peter Fleischer
  • Pavel Cudlín
Original Article
  • 249 Downloads

Abstract

Ectomycorrhizal (ECM) fungi, as symbionts of many tree species in temperate forests, are thought to play an important role in forest regeneration processes after large disturbances. Their reaction to different disturbance and management regimes was studied in spruce forests (Lariceto-Piceetum) 10 years after a severe windthrow in the Tatra National Park (Slovak Republic). ECM community structure was compared between different “management types″—cleared area (EXT), area affected by wildfire (FIRE), uncleared area left for natural development (NEX), and mature forest as a control (REF). Based on Illumina sequencing of soil samples, we determined that the percentage of sequences assigned to ECM fungi decreased with increasing disturbance and management intensity (REF → NEX → EXT → FIRE). Similarly, the total number of ECM species per each of ten sampling points per plot (100 ha) differed between managed (EXT-11 species, FIRE-9) and unmanaged (NEX-16, REF-14) treatments. On the other hand, the percentage of sequences belonging to ericoid mycorrhizal fungi increased. Management type significantly influenced the composition of the ECM community, while vegetation and soil characteristics explained less data variation. The ECM species assemblage of the unmanaged site (NEX) was the most similar to the mature forest, while that of the burnt site was the most different. Thelephora terrestris dominated in all treatments affected by windthrow, accompanied by Tylospora fibrillosa (NEX) and Tylospora asterophora (EXT and FIRE). Management regime was also the most important factor affecting ECM species composition on the roots of spruce seedlings assessed by Sanger sequencing.

Keywords

Ectomycorrhiza Windthrow Fire Picea abies Forest regeneration Next generation sequencing Fungi 

Notes

Acknowledgements

This work was supported by the Ministry of Education, Youth and Sports of CR within the National Sustainability Program I, grant No. LO1415 and grant No. LD 15044. We thank Filip Holub, Jan Purkyt, Martin Čermák, Ondřej Cudlín, Jan Červenka, and students from the Faculty of Forestry, Mendel University in Brno for field and lab assistance. Petra Havlíčková helped with the amplicon sequencing.

Supplementary material

572_2018_820_MOESM1_ESM.xlsx (56 kb)
ESM 1 (XLSX 55 kb)
572_2018_820_MOESM2_ESM.jpg (3.7 mb)
ESM 2 Proportion of the most abundant ECM species at each sampling point (JPEG 3740 kb)
572_2018_820_MOESM3_ESM.pdf (192 kb)
ESM 3 (PDF 191 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Botany, Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  2. 2.Global Change Research InstituteCzech Academy of SciencesČeské BudějoviceCzech Republic
  3. 3.Faculty of ForestryTechnical University in ZvolenZvolenSlovakia
  4. 4.Research Station of TANAP, State Forest of TANAPTatranská LomnicaSlovakia

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